Rabbit anti phospho cdc2 tyr15

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-phospho-CDC2 (Tyr15) is an antibody that specifically recognizes the phosphorylated form of CDC2 (also known as CDK1) at tyrosine 15. This antibody can be used to detect the phosphorylation status of CDC2 in various experimental applications.

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2 protocols using rabbit anti phospho cdc2 tyr15

Western Blotting and Cell Fractionation

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Western blotting was performed as already described^{55 (link)}. The following antibodies were used: mouse anti-GLI1 (#2643), mouse anti-cyclin A2 (#4656), rabbit anti-BCL2 (#2876), rabbit anti-BAX (#2772), rabbit anti-cyclin B1 (#12231), rabbit anti-PARP-1 (#9532), rabbit anti-phospho-ATR (Ser428) (#2853), rabbit anti-phospho-CHK1 (Ser345) (#2348), rabbit anti-phospho-CDC2 (Tyr15) (#4539), rabbit anti-phospho-H2A.X (Ser139) (#9718), rabbit anti-phospho-Histone H3 (Ser10) (#3377), rabbit anti-phospho-WEE1 (Ser642) (#4910) (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-CDC2 (sc-954), mouse anti-Myc (sc-40), mouse anti-HSP90 (sc-13119) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and rabbit anti-SMO (ST1718) (Merck Millipore, Burlington, MA, USA). Chemiluminescent detection was used. Cell fractionation was performed as previously described^{56 (link)}. The following antibodies were used: mouse anti-GLI1 (#2643) (Cell Signaling Technology), goat anti-fibrillarin (D-14), and goat anti-GAPDH (V-18) (Santa Cruz Biotechnology).

Pietrobono S., Santini R., Gagliardi S., Dapporto F., Colecchia D., Chiariello M., Leone C., Valoti M., Manetti F., Petricci E., Taddei M, & Stecca B. (2018). Targeted inhibition of Hedgehog-GLI signaling by novel acylguanidine derivatives inhibits melanoma cell growth by inducing replication stress and mitotic catastrophe. Cell Death & Disease, 9(2), 142.

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Checkpoint Kinase 2 Activation in Cell Cycle Regulation

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The primary antibodies used for western blot analysis were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA) and were as follows: Rabbit anti-checkpoint kinase 2 (Chk2) (catalog no. 2662S; dilution, 1:1,000); rabbit anti-phospho-Chk2 (Thr68) (catalog no. 2661S; dilution, 1:1,000); rabbit anti-cell division control (cdc) 25c (catalog no. 4688S; dilution, 1:1,000); rabbit anti-phospho-cdc25c (Thr48) (catalog no. 9527S; dilution, 1:1,000); mouse anti-cdc2 (catalog no. 9116S; dilution, 1:1,000); rabbit anti-phospho-cdc2 (Tyr15) (catalog no. 9111S; dilution, 1:1,000); and rabbit anti-cyclin B1 (catalog no. 4138S; dilution, 1:1,000). Mouse anti-PARP1 (catalog no. sc-8007; immunohistochemistry dilution, 1:50; western blotting dilution, 1:1,000) was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Secondary antibodies used for western blot analysis were obtained from GE Healthcare Life Sciences (Little Chalfont, UK) and were as follows: Horseradish peroxidase (HRP)-conjugated sheep anti-mouse IgG (catalog no. NA931; dilution, 1:100,000) and HRP-conjugated donkey anti-rabbit IgG (catalog no. NA934; dilution, 1:100,000).

Yamamoto M., Yamasaki M., Tsukao Y., Tanaka K., Miyazaki Y., Makino T., Takahashi T., Kurokawa Y., Nakajima K., Takiguchi S., Mori M, & Doki Y. (2017). Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma. Oncology Letters, 14(2), 1581-1587.

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