Folic acid

Manufactured by Sinopharm

Sourced in China

Folic acid is a water-soluble B-complex vitamin. It is an essential nutrient that plays a vital role in various bodily functions.

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Lab products found in correlation

11 protocols using folic acid

Chitosan-Based Nanoparticles for Drug Delivery

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Chitosan with an average molecular weight (MW) of 200 kDa and degree of deacetylation of 85% was obtained from Qingdao Baicheng Biochemical Corp. (Qingdao, China). The materials, including 3-chloro-2-hydroxypropyltrimethyl ammonium chloride, sodium tripolyphosphate (TPP), folic acid, adriamycin hydrochloride, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Isopropyl alcohol, sodium hydroxide, and ethanol were provided by Sigma-Aldrich Chemical Corp. (Shanghai, China). All the chemical reagents and solvents were obtained from commercial sources and used as received.

Mi Y., Zhang J., Zhang L., Li Q., Cheng Y, & Guo Z. (2021). Synthesis, Characterization, and Evaluation of Nanoparticles Loading Adriamycin Based on 2-Hydroxypropyltrimethyl Ammonium Chloride Chitosan Grafting Folic Acid. Polymers, 13(14), 2229.

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Folic Acid Analytical Reagent Protocol

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All chemicals are analytical reagent grade and were used as received. Folic acid is a biological reagent purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China.

Li H., Li Z., Zhao J., Tang B., Chen Y., Hu Y., He Z, & Wang Y. (2014). Carboxymethyl chitosan-folic acid-conjugated Fe3O4@SiO2 as a safe and targeting antitumor nanovehicle in vitro. Nanoscale Research Letters, 9(1), 146.

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Targeted Chitosan-based Magnetic Nanoparticles

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Chitosan (molecular weight =23 kDa; 90%–95% deacetylated) was obtained from Shanghai Bio Science & Technology Co., Ltd. (Shanghai, People’s Republic of China). Palmitic acid and acetic anhydride were purchased from Guangzhou Howei Chemical Co., Ltd. (Guangzhou, People’s Republic of China). Folic acid was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). SPIONs (Fe₃O₄; mean size: 5 nm) and MTT bromide were obtained from Sigma-Aldrich Co., Ltd. (St Louis, MO, USA). The reagent 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) was provided by Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and reagents used were of analytical grade. Female athymic mice (4–6 weeks old; 15–20 g) were obtained from the Experimental Animal Center of Southern Medical University (Guangzhou, People’s Republic of China), housed in cages, and fed standard laboratory feed and water.

Xiao Y., Lin Z.T., Chen Y., Wang H., Deng Y.L., Le D.E., Bin J., Li M., Liao Y., Liu Y., Jiang G, & Bin J. (2015). High molecular weight chitosan derivative polymeric micelles encapsulating superparamagnetic iron oxide for tumor-targeted magnetic resonance imaging. International Journal of Nanomedicine, 10, 1155-1172.

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Cytotoxicity Evaluation of Pluronic F127

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Pluronic F127 and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich. Oleic acid, oleylamine, benzyl alcohol and folic acid were acquired from Sinopharm Chemical Reagent Co., Ltd. HepG2 (liver hepatocellular carcinoma) cells were acquired from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China.

Fei M.Y., Song M.M., Wang P., Pang G.Z., Chen J., Lu D.P., Liu R., Zhang G.Y., Zhao T.T., Shen Y.X, & Yu Y.Q. (2020). Folic acid modified Fe3O4 nanoclusters by a one-step ultrasonic technique for drug delivery and MR imaging. RSC Advances, 10(9), 5294-5303.

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Osteogenic Differentiation of MC3T3-E1 Cells

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MC3T3-E1 cells (Type Culture Collection of the Chinese Academy of Sciences), usually cultured in 90% α-MEM media (cat. no. 11900024; Gibco; Thermo Fisher Scientific, Inc.) containing 1.5 g/l NaHCO₃, 43.2 mg/l inositol (Sinopharm Chemical Reagent Co., Ltd.), 8.82 mg/l folic acid (Sinopharm Chemical Reagent Co., Ltd.) and 7.8 mg/l β-mercaptoethanol (Sigma-Aldrich; Merck KGaA) and 10% FBS at 37˚C and 5% CO₂. They were seeded into 12-well plates at a density of 5x10⁴ cells/well. Osteogenic induction medium (300 µl; cat. no. PH-B-002; Puhe Biotechnology Co., Ltd.; https://puhe.biomart.cn/) containing the PLLA/PLGA/PCL nano-scaffolds, PLGA-PLLA/PLGA/PCL composite scaffolds and BMP-2-PLGA-PLLA/PLGA/PCL composite scaffolds was then added to the cells, followed by incubation for 14 days at 37˚C. The medium was removed at the 14th day. The cells were washed twice with PBS, fixed in 4% polyformaldehyde at 4˚C for 4 h, washed once with deionized water and stained with 2% alizarin red (20 mg/ml, pH=4.1-4.3) at room temperature for 20 min. Any extra alizarin red was subsequently removed using deionized water followed by fluorescent microscopy (magnification: x200; Model: IX71; Olympus Corporation).

Zhu H., Shi Z., Cai X., Yang X, & Zhou C. (2020). The combination of PLLA/PLGA/PCL composite scaffolds integrated with BMP-2-loaded microspheres and low-intensity pulsed ultrasound alleviates steroid-induced osteonecrosis of the femoral head. Experimental and Therapeutic Medicine, 20(6), 126.

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Folate-Targeted Lipid Nanoparticles for siRNA Delivery

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UA and folate-PEG-CHEMS were obtained from the pharmacy school of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China (purity >95%). 1, 2-dioleoyl-3-trimethylammonium-propane (Chloride Salt) (DOTAP) was purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Monomethoxy polyethylene glycol 2000-distearoyl phosphatidylethanolamine (MPEG-DSPE2000) was obtained from Lipoid GmbH (Germany). Cholesterol (CHOL) was purchased from Acros Organics. Calcein was obtained from Aladdin Chemistry Co. Ltd. Folic acid was purchased from Sinopharm Chemical Reagent Co., Ltd. RPMI 1640 media were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Methyl thiazolyl-diphenyl-tetrazolium bromide (MTT). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biological Technology Co., Ltd., Hangzhou, China. Female Kunming mice (25 g, 6–8 weeks old) and female Balb/c nude mice (20 g, 6–8 weeks old) were obtained from Beijing Huafukang Bioscience Technology Co. (Beijing, China; license SCXK (jing) 2009-0004). Cy3-labeled Bmi1 siRNA were synthesized by Ribobio Co., Ltd (Guangzhou, China). The Bmi1 siRNA sequences for mice were sense strand 5'-CCA GAC CAC UCC UGA ACA UTT-3' and anti-sense strand 5'-AUG UUC AGG AGU GGU CUG GTT-3', and for human were sense strand 5'-CCA GAC CAC UAC UGA AUA UAA-3' and anti-sense strand 5'-UUA UAU UCA GUA GUG GUC UGG UU-3'.

Li W., Yan R., Liu Y., He C., Zhang X., Lu Y., Khan M.W., Xu C., Yang T, & Xiang G. (2019). Co-delivery of Bmi1 small interfering RNA with ursolic acid by folate receptor-targeted cationic liposomes enhances anti-tumor activity of ursolic acid in vitro and in vivo. Drug Delivery, 26(1), 794-802.

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Synthesis and Evaluation of Multifunctional Nanoparticles

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Folic acid (FA), β-cyclodextrin (β-CD), sodium periodate, dichloromethane (DCM), diethyl ether, tetrahydrofuran (THF), dioxane, benzoyl peroxide (BPO), triethylamine (TEA), and dimethylsulfone (DMSO) were obtained from Sinopharm Chemical Reagent Co., Ltd, China. N-hydroxysuccinimide (NHS), acryloyl chloride and p-aminoazobenzene (AZO) were purchased from Aladdin. Trypsin, Dulbecco's modified Eagle's medium (DMEM), Camptothecin (CPT) and 3-(4, 5-dimethyl) thiazol-2,5-dimethyl tetrazolium bromide (MTT) were obtained from Sigma. Fetal bovine serum (FBS) was purchased from Sijiqing biotech. Co., China. All other reagents and solvents were of analytical grade and used as received.

Pang J., Gao Z., Tan H., Mao X., Xu J., Kong J, & Hu X. (2019). Fabrication, Investigation, and Application of Light-Responsive Self-Assembled Nanoparticles. Frontiers in Chemistry, 7, 620.

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Electrochemical Sensing of Biomolecules

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All reagents are analytical reagents, and they were used without further purification. Specifically, CuSO₄·5H₂O, Na₂SO₄, NiSO₄·6H₂O, aqueous ammonia (25–28%), NiCl₂·6H₂O, C₈H₆O₄, N, N-dimethylacetamide (DMF), D-(+)-Glucose, dopamine (DA), L-ascorbic acid (AA), acetaminophen, fructose, sucrose, folic acid, and l-cysteine were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Additionally, K₂S₂O₈ was purchased from Tianjin Kaitong Chemical Reagent Co., Ltd. (Tianjin, China). Uric acid (UA) was purchased from Alfa Aesar.

Zhang L., Ye C., Li X., Ding Y., Liang H., Zhao G, & Wang Y. (2017). A CuNi/C Nanosheet Array Based on a Metal–Organic Framework Derivate as a Supersensitive Non-Enzymatic Glucose Sensor. Nano-Micro Letters, 10(2), 28.

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Gelatin-based Nanoparticle Synthesis

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Gelatin type B was obtained
from Aladdin Chemistry Co. Ltd. (Shanghai). Carbonate–bicarbonate
(CB) buffer and D₂O were obtained from Beijing InnoChem
Science & Technology Co. Ltd. CuCl₂·2H₂O, poly(vinylpyrrolidone), Na₂S·9H₂O,
sodium citrate (C₆H₅Na₃O₇), folic acid, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
(EDC), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride,
dimethyl sulfoxide (DMSO), 4% paraformaldehyde fixing solution, 0.25%
trypsin, and glutaraldehyde were purchased from Sinopharm Chemical
Reagent Co., Ltd. Dulbecco’s modified Eagle’s medium
(DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were
obtained from Gibco (Thermos Fisher Scientific). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenylte-trazolium
bromide (MTT), 4′,6-diamidine-2′-phenylindole dihydro-chloride
(DAPI), 2′-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole, trihydrochloride (Hoechst 33342), and propidium
iodide (PI) were purchased from Beijing Solarbio Bio-Tech Co. Ltd.
(China). All other reagents used in this research were of analytical
grade.

Xia Y., Xu R., Ye S., Yan J., Kumar P., Zhang P, & Zhao X. (2023). Microfluidic Formulation of Curcumin-Loaded Multiresponsive Gelatin Nanoparticles for Anticancer Therapy. ACS Biomaterials Science & Engineering, 9(6), 3402-3413.

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Synthesis and Characterization of DDTC-Ag Nanoparticles

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Diethyldithiocarbamic acid silver salt (AgDDTC, 98%), dodecanethiol (DT, 98%), n-hexane, acetone, trichloromethane, dimethyl sulfoxide (DMSO), folic acid (FA, 97%) and pyridine were purchased from Sinopharm Group Chemical Reagent Co., Ltd. PEG-phospholipid (DSPE-PEG₂₀₀₀), amino-PEG-phospholipid (DSPE-PEG₂₀₀₀-NH₂) were purchased from Avanti, lecithin (98%), polyoxyethylene stearate were purchased from Aladdin, octadecene (ODE, 90%) was purchased from Aldrich. All reagents were used directly without further purification.
The equipments used in the experiments included UV-2550 UV–Vis spectrophotometer (Shimadzu, Japan), Tecnai G20 U-Twin High Resolution Transmission Electron Microscope (FEI, Netherlands), Nano-ZS90 Nanometer Size Meter (Malvern, UK), Ni-E Positive Slice Scanning Microscope (Nikon, Japan), SP-4430 Dry Biochemical Analyzer (Arkary, Japan), Centrifugal Concentrator (Ependorf, Germany), Elx-808 Microplate Reader (Biotek, USA), CA-700 Automatic Blood Analyzer (STAC, China), MDL-III-808-2.5 W Laser (Changchun New Industries Optoelectronics Tech. CO., LTD., China), EasIR-9 Thermal Imager (Wuhan Guide Infrared Co., LTD, China), IX71 Fluorescence Microscope (Olympus, Japan). Near infrared FI system [30 (link)] and PAI system [31 (link), 32 (link)] were homemade by the laboratory.

Zhang X.S., Xuan Y., Yang X.Q., Cheng K., Zhang R.Y., Li C., Tan F., Cao Y.C., Song X.L., An J., Hou X.L, & Zhao Y.D. (2018). A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo. Journal of Nanobiotechnology, 16, 42.

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