Liver tissue was homogenized in TRIZOL buffer (Invitrogen; Carlsbad, CA) and RNA isolated according to manufacturer's protocol. RT-PCR was performed using TaqMan primers (Invitrogen; Carlsbad, CA) and qScript 1-Step Fast qRT-PCR Kit (Quanta Biosciences; Gaithersburg, MD) on an iCycler thermal cycler (BioRad; Hercules, CA).
Qscript 1 step fast qrt pcr kit
Manufactured by Quanta Biosciences
Sourced in Canada
The QScript 1-Step Fast qRT-PCR Kit is a one-step reverse transcription and quantitative real-time PCR (qRT-PCR) reagent system. It is designed for the rapid and sensitive detection and quantification of RNA targets.
Automatically generated - may contain errors
2 protocols using qscript 1 step fast qrt pcr kit
1
RNA Extraction and qRT-PCR Analysis
2
RNA Extraction and qRT-PCR Analysis
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Liver tissue was homogenized in TRIZOL buffer (Invitrogen; Carlsbad, CA) and RNA isolated according to manufacturer's protocol. RT-PCR was performed using TaqMan primers (Invitrogen; Carlsbad, CA) and qScript 1-Step Fast qRT-PCR Kit (Quanta Biosciences; Gaithersburg, MD) on an iCycler thermal cycler (BioRad; Hercules, CA).
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