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Tc treated round bottomplates

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TC-treated round-bottom plates are a type of laboratory equipment used for various cell culture applications. These plates feature a round-bottom design and are treated with a tissue culture (TC) surface to promote cell attachment and growth. The core function of these plates is to provide a suitable environment for culturing cells in a controlled laboratory setting.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by American Type Culture Collection (2 mentions)

2 protocols using tc treated round bottomplates

1

MCF-7 Cell Growth Inhibition Assay

Cited 1 time
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The MCF-7 cell growth
inhibition assay was carried out as described previously.45 (link) A broth microdilution method was used to determine
the growth inhibition IC50 values. Compounds were half-log
serial diluted using cell culture media into 96-well TC-treated round-bottom
plates (Corning Inc., Corning, NY). Cells were plated at a density
of 5000 cells/well and then incubated under the same culture conditions
for 2 days at which time an MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide) cell proliferation assay (ATCC, Manassas, VA) was performed
to obtain dose–response curves.
Li K., Schurig-Briccio L.A., Feng X., Upadhyay A., Pujari V., Lechartier B., Fontes F.L., Yang H., Rao G., Zhu W., Gulati A., No J.H., Cintra G., Bogue S., Liu Y.L., Molohon K., Orlean P., Mitchell D.A., Freitas-Junior L., Ren F., Sun H., Jiang T., Li Y., Guo R.T., Cole S.T., Gennis R.B., Crick D.C, & Oldfield E. (2014). Multitarget Drug Discovery for Tuberculosis and Other Infectious Diseases. Journal of Medicinal Chemistry, 57(7), 3126-3139.
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2

MCF-7 Cell Growth Inhibition Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The MCF-7 cell growth inhibition assay was carried out as described previously45 (link). A broth micro-dilution method was used to determine the growth inhibition IC50 values. Compounds were half-log serial diluted using cell culture media into 96-well TC-treated round bottom plates (Corning Inc., Corning, NY). Cells were plated at a density of 5000 cells/well and then incubated under the same culture conditions for 2 days at which time an MTT ((3-(4,5-dimethylthi-azole-2-yl)-2,5-diphenyltetrazolium bromide) cell proliferation assay (ATCC, Manassas, VA) was performed to obtain dose response curves.
Li K., Schurig-Briccio L.A., Feng X., Upadhyay A., Pujari V., Lechartier B., Fontes F.L., Yang H., Rao G., Zhu W., Gulati A., No J.H., Cintra G., Bogue S., Liu Y.L., Molohon K., Orlean P., Mitchell D.A., Freitas-Junior L., Ren F., Sun H., Jiang T., Li Y., Guo R.T., Cole S.T., Gennis R.B., Crick D.C, & Oldfield E. (2014). Multitarget Drug Discovery for Tuberculosis and Other Infectious Diseases. Journal of Medicinal Chemistry, 57(7), 3126-3139.
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