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L type tg m

Manufactured by Fujifilm
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Sourced in Holy See (Vatican City State), United States

The L-Type TG M is a laboratory equipment product from Fujifilm. It is designed for specific technical functions, but a detailed description without interpretation or extrapolation cannot be provided while maintaining an unbiased and factual approach. Further information is not available.

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Lab products found in correlation

Infinity cholesterol reagent, by Thermo Fisher Scientific (1 mentions) Nefa hr 2, by Fujifilm (1 mentions) Human apolipoprotein b elisa kit, by Mabtech (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Infinity tg assay tr22421, by Thermo Fisher Scientific (1 mentions) Aspartate aminotransferase, by Merck Group (1 mentions) Nefa c, by Fujifilm (1 mentions) Mouse il 1ra il 1f3 quantikine elisa kit, by R&D Systems (1 mentions)

Close spelling variants of this product

L type Wako TG-M or NEFA HF (2 citations) L-Type TG M assay (4 citations) L-Type TG M reagents (2 citations) Wako L-Type TG M (9 citations) L-Type TG M kit (9 citations) L-Type TG M test (3 citations) Wako L-Type TG M kit (2 citations)

7 protocols using l type tg m

1

Quantifying Plasma Lipid Profiles

Cited 3 times
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Total plasma triacylglycerol levels (L-Type TG M, Wako Diagnostics) and total plasma cholesterol levels were quantified enzymatically (Infinity Cholesterol Reagent, Thermo/Fisher). The distribution of cholesterol across lipoprotein classes was performed by fast-protein liquid chromatography (FPLC) using tandem superose-6 HR columns coupled with an online enzymatic cholesterol quantification as previously described (Warrier et al., 2015 (link); Brown et al., 2010 (link); Brown et al., 2008a (link); Brown et al., 2008b (link); Izem and Morton, 2009 (link); Helsley et al., 2016 (link)).
Helsley R.N., Varadharajan V., Brown A.L., Gromovsky A.D., Schugar R.C., Ramachandiran I., Fung K., Kabbany M.N., Banerjee R., Neumann C.K., Finney C., Pathak P., Orabi D., Osborn L.J., Massey W., Zhang R., Kadam A., Sansbury B.E., Pan C., Sacks J., Lee R.G., Crooke R.M., Graham M.J., Lemieux M.E., Gogonea V., Kirwan J.P., Allende D.S., Civelek M., Fox P.L., Rudel L.L., Lusis A.J., Spite M, & Brown J.M. (2019). Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease. eLife, 8, e49882.
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2

Serum Biomarkers Assay Protocol

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Serum leptin was measured with ELISA kits for mouse Leptin (EMD Millipore, Billerica, MA). The sensitivity range was from 0.2 ng/ml to 30 ng/ml and the sensitivity limit was 0.05 ng/ml. Serum triglycerides (TG) were measured using a colorimetric assay kit (L-type TG M, Wako, Mountain View, CA). The range of the standard curve was 0–107 mg/dL, and linearity continued through 2000 mg/dL. Serum was also analyzed for cholesterol (Pointe Scientific, Inc., Canton, MI), and nonesterified fatty acids (NEFA-HR (2), Wako, Mountain View, CA).
Rubin B.S., Paranjpe M., DaFonte T., Schaeberle C., Soto A.M., Obin M, & Greenberg A.S. (2016). Perinatal BPA exposure alters body weight and composition in a dose specific and sex specific manner: The addition of peripubertal exposure exacerbates adverse effects in female mice. Reproductive toxicology (Elmsford, N.Y.), 68, 130-144.
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3

Differentiated Cell Lipid Metabolism

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24 hour spent culture media from differentiating cells (one million cells per well) at indicated time points (day 0, day 4, day 10 and day 15 post-differentiation) were collected. The amount of ApoB-100 (ng/ml) produced by the cells was measured by the Human Apolipoprotein B ELISA kit (MABTECH, Inc). Triglycerides (mg/ml) were measured using colorimetric assay (L-Type TG M, Wako Diagnostics). Fold change value to day 0 (undifferentiated cells) was obtained by calculating the ratio with that of each time point.
Irudayam J.I., Contreras D., Spurka L., Subramanian A., Allen J., Ren S., Kanagavel V., Nguyen Q., Ramaiah A., Ramamoorthy K., French S.W., Klein A.S., Funari V, & Arumugaswami V. (2015). Characterization of Type I Interferon pathway during Hepatic Differentiation of Human Pluripotent Stem Cells and hepatitis C virus infection. Stem cell research, 15(2), 354-364.
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4

Extraction and Quantification of Cellular Lipids

Cited 1 time
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Cells were washed with ice-cold 1X Dulbecco’s phosphate-buffered saline with no added magnesium or calcium (DPBS, Gibco) twice and lipid-extracted with hexane:isopropanol (3:2, vol:vol) overnight at room temperature. The lipid extracts were dried under a nitrogen stream at 60°C. Once dry, 1% Triton-X in chloroform was added and then dried down again. The residue was re-suspended in ddH2O and heated at 60°C for 1 h to yield an aqueous lipid extract for each sample. The TAG levels were measured using an enzymatic assay (Wako Diagnostics L-Type TG M) according to manufacturer instructions. After lipid extraction, cells were dissolved with 0.1 N of NaOH, and protein concentration was measured using a Pierce™ BCA Protein Assay Kit (Life Technologies) for protein normalization (22 (link)).
Seramur M.E., Sink S., Cox A.O., Furdui C.M, & Key C.C. (2023). ABHD4 regulates adipocyte differentiation in vitro but does not affect adipose tissue lipid metabolism in mice. Journal of Lipid Research, 64(8), 100405.
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5

Plasma Lipid Profiling Protocol

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After a 16-h fast, blood was collected into tubes containing EDTA for the separation of plasma. Kits (Determiner TC II, Kyowa Medex and L-Type TG M, Wako) were used to determine the plasma levels of total cholesterol (TC) and triglyceride (TG) enzymatically. High performance liquid chromatography (HPLC) analyses of plasma were performed, as described previously14 (link)).
Yamazaki H., Takahashi M., Wakabayashi T., Sakai K., Yamamuro D., Takei A., Takei S., Nagashima S., Yagyu H., Sekiya M., Ebihara K, & Ishibashi S. (2019). Loss of ACAT1 Attenuates Atherosclerosis Aggravated by Loss of NCEH1 in Bone Marrow-Derived Cells. Journal of Atherosclerosis and Thrombosis, 26(3), 246-259.
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6

Liver and Plasma Triglyceride Quantification

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Liver TG content was measured as previously described [41 (link)]. Briefly, lipids were extracted from liver samples (~50–100 mg) using chloroform:methanol (vol:vol = 2:1). Next, sulphuric acid (0.05 %) was added to split the phase. After centrifugation (1000 × g for 5 min at room temperature), the bottom/organic phase was transferred to a new glass tube and triton X-100 (1% in chloroform) was added. Samples were dried down at 60°C under nitrogen gas and water was added into each sample (1 ml of 1% Triton X-100 in chloroform: 0.5 ml of water). Liver TGs were measured using commercial enzymatic reagents (Wako Diagnostics L-Type TG M; Richmond, VA). Plasma TG content was determined using a commercial assay from Thermo Scientific (Infinity TG assay #TR22421 and TG standards #TG22923; Norcross, GA).
Collins B., Hoffman J., Martinez K., Grace M., Lila M.A., Cockrell C., Nadimpalli A., Chang E., Chuang C.C., Zhong W., Mackert J., Shen W., Cooney P., Hopkins R, & McIntosh M. (2016). A Polyphenol-Rich Fraction Obtained from Table Grapes Decreases Adiposity, Insulin Resistance, and Markers of Inflammation and Impacts Gut Microbiota in High-Fat Fed Mice. The Journal of nutritional biochemistry, 31, 150-165.
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7

Cytokine and Metabolic Biomarker Measurement

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IL-1Ra serum levels were measured using a Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA). Plasma concentrations of Interleukin 10 (IL-10), Interleukin 1 beta (IL-1β), Interleukin 6 (IL-6), Monocyte chemotactic protein 1 (MCP-1) and Tumor necrosis factor alpha (TNF-mor necrosis factorleukin 10 (IL-10), Interleukin 1 beta (ILBillerica, MA, USA). Plasma insulin levels were measured by ELISA (ALPCO diagnostics, USA). Plasma free fatty acid (FFA) levels were measured enzymatically using a commercially available kit (NEFA C; Wako Chemicals USA). Triglyceride (TG) levels were measured enzymatically using a commercially available kit (L-Type TG M, Wako Chemicals, USA). Liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST) and glutamate dehydrogenase (GDH) were measured using assay kits (Sigma, MO, USA).
Franck N., Maris M., Nalbandian S., Talukdar S., Schenk S., Hofmann H.P., Bullough D, & Osborn O. (2014). Knock-Down of IL-1Ra in Obese Mice Decreases Liver Inflammation and Improves Insulin Sensitivity. PLoS ONE, 9(9), e107487.
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