450 nm filters

Vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses were generated in HEK293FT cells. Cells were seeded in 6-well plates at 1 × 10⁶ cells/mL and directly transfected with pLemir-NS [64 (link)] for RFP delivery, psPAX2 for viral capsid proteins and pCMV-VSV-G [65 (link)] for viral envelope proteins (summarized plasmids are listed in Table 2). After 24 h, medium was collected every 24 h for 120 h in total. After 5 days, medium was centrifuged for 30 min at 2000× g, filtered through 450 nm filters (Sartorius, Göttingen, Germany) and concentrated in Vivaspin columns (Sartorius, Göttingen, Germany).

Vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses were generated in HEK293FT cells. Cells were seeded in 6-well plates at 1 × 10⁶ cells/mL and directly transfected with pLenti CMV GFP Neo [22 (link)] for GFP delivery, psPAX2 for viral capsid proteins, and pCMV-VSV-G [22 (link)] for viral envelope proteins (summarized plasmids are listed in Table 1). Also, 16 h after transfection, sodium butyrate containing medium (0.01 M) was added. After 8 h, medium without sodium butyrate was used and collected every 24 h. After 5 days, medium was centrifuged for 30 min at 2000× g, filtered through 450 nm filters (Sartorius, Germany), and concentrated via Vivaspin columns (Sartorius, Göttingen, Germany).