The following antibodies were purchased: for Western blotting, anti-β-catenin (1:5000; Abcam, cat. no. ab32572), anti-β-actin (1:5000; Bioss, cat. no. bs-0061R), anti-CDK5RAP2 (1:2000; Abcam, cat. no. ab70213), anti-survivin (1:2000; Abclonol, cat. no. A1551), anti-ALDH1 (1:1000; Cell Signaling Technology, cat. no. 54135), anti-Notch1 (1:1000, Abcam, cat. no. ab52627), anti-EZH2 (1:1000; Cell Signaling Technology, cat. no. 5246), anti-CCND1 (1:1000, Cell Signaling Technology, cat. no. 55506), and horseradish peroxidase-conjugated secondary antibodies (1:1000; Cell Signaling Technology, Inc.); for IHC analyses, anti-CDK5RAP2 (1:400; Abcam, cat. no. ab235893), anti-ALDH1 (1:50; Abcam, cat. no. ab52492), anti-SOX2 (1:100; Abcam, cat. no. Ab92494), anti-CD44 (1:4000; Abcam, cat. no. ab189524), anti-CD133 (1:1000; Abcam, cat. no. ab222782), anti-Notch1 (1:150; Abcam, cat. no. ab52627), anti-EZH2 (1:200; Cell Signaling Technology, cat. no. 5246), and anti-CCND1 (1:200; ABclonal, cat. no. A19038); and for immunofluorescence analyses, anti-α-tubulin (1:500, YL1/2; Santa Cruz Biotechnology, cat. no. sc-53029), anti-γ-tubulin (1:1000, GTU88; Sigma-Aldrich, cat. no. T5326), and Alexa Fluor-conjugated secondary antibodies (1:500; Thermo Fisher Scientific).
Anti cdk5rap2
Manufactured by Abcam
Anti-CDK5RAP2 is an antibody that recognizes the CDK5RAP2 protein. CDK5RAP2 is a centrosome-associated protein involved in microtubule organization and cell cycle regulation. This antibody can be used for applications such as Western blotting, immunoprecipitation, and immunocytochemistry to detect and study the CDK5RAP2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd133, by Abcam (1 mentions)
Anti notch1, by Abcam (1 mentions)
Anti ccnd1, by Cell Signaling Technology (1 mentions)
Anti sox2, by Abcam (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Anti γ tubulin, by Merck Group (1 mentions)
Anti aldh1, by Cell Signaling Technology (1 mentions)
Anti aldh1, by Abcam (1 mentions)
Anti cd44, by Abcam (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Anti ezh2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Bioss Antibodies (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Anti pericentrin, by Abcam (1 mentions)
Hpa006778, by Merck Group (1 mentions)
Anti cep152, by Abcam (1 mentions)
Anti γ tubulin gtu 88, by Merck Group (1 mentions)
Anti cpap, by Proteintech (1 mentions)
Anti α tubulin dm1a, by Merck Group (1 mentions)
2 protocols using anti cdk5rap2
1
Comprehensive Antibody Panel for Cellular Analyses
2
Antibody Protocols for Centrosomal Proteins
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Antibodies against Cep192 (1–211 aa; used at 0.5 µg/ml), SAS-6 (501–657 aa; used at 0.5 µg/ml), and Plk4 (501–657 aa; used at 1 µg/ml) were previously described (Wong et al., 2015 (link)). The following antibodies were purchased from commercial sources, with their working concentrations indicated in parentheses: anti-Trim37 (1:2,000 for Western blotting; A301-174A; Bethyl Laboratories, Inc.), anti-Usp28 (1:1,000 for Western blot; ab126604; Abcam), anti-Usp28 (1:100 for immunofluorescence; HPA006778; Sigma-Aldrich), anti-53BP1 (1:5,000), anti-Cep152 (1:2,000; Abcam), anti-Cdk5rap2 (1:4,000; Abcam), GTU-88 (anti–γ-tubulin; 1:1,000; Sigma-Aldrich), anti-pericentrin (1 µg/ml; Abcam), anti-CPAP (1:400; Proteintech), anti-p53 (1:100 for Western blot; OP43; EMD Millipore), anti-p53 (1:500 for immunofluorescence; OP140; EMD Millipore), anti-p21 (1:1,000; #2947; Cell Signaling Technology), DM1A (anti–α-tubulin; 1:5,000; Sigma-Aldrich), anti-GAPDH (1:1,000; 14C10; Cell Signaling Technology), PCM-1 (1:400; #5259; Cell Signaling Technology), and Fab fragment (goat anti–rabbit IgG; 30 µg/ml; Jackson ImmunoResearch Laboratories, Inc.). Secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc.
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