Trypan blue powder

Synthetic human Aβ (1-42) peptides (Aβ42) were purchased from Chinapeptide (Shanghai, China). The DAPK1-specific inhibitor (4Z)-4-(3-pyridylmethylene)-2-styryl-oxazol-5-one (C6) was acquired from Calbiochem (California, United States). The HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin (tanespimycin, 17-AAG) was from Macklin (Shanghai, China). DMSO, trypan blue powder, 1, 1, 1, 3, 3, 3-hexafluoro-isopropanol (HFIP), cycloheximide (CHX), poly-D-lysine and cytosine β-D-arabinofuranoside were purchased from Sigma-Aldrich (Missouri, United States). 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), nonfat milk powder and bovine serum albumin (BSA) were from Sangon (Shanghai, China). Hoechst 33342 solution was purchased from Beyotime (Shanghai, China). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), sodium pyruvate, GlutaMAX™ Supplement, Hank's balanced salt solution (HBSS), glucose solution, trypsin and penicillin-streptomycin were provided by Gibco (Texas, United States). Neurobasal medium and the B-27 supplement were obtained from BasalMedia (Shanghai, China). The anti-phospho-Ser71-Pin1 (pS71-Pin1) antibody was prepared and purified by immunizing rabbits with a peptide sequence (CSQSRRPSSWR) of Pin1 containing the pS71 residue (Abmart, Shanghai, China).

Polylactic acid (PLA), Diethylenetriamine, 25% Glutaraldehyde stock solution, and trypan blue powder were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Quick Start™ Bradford 1× Dye Reagent was purchased from Bio-Rad (Hercules, CA, USA). 4-Methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy-β-d-glucopyranoside (MUGS) was purchased by Toronto Research Chemicals (Toronto, ON, Canada). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Trypsin, and Penicillin/Streptomycin were purchased from Euroclone (Pero, Italy). HexA polyclonal antibody and secondary antibody conjugated with Alexa Fluor^® 488 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Vectashield^® Vibrance™ Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Vector Laboratories Inc. (Burlingame, CA, USA).

The synthetic procedures of o-PTZ, m-PTZ, and p-PTZ compounds have been already described
in a previous paper.^{29 (link)} Spectral and photophysical
characterizations were performed in several solvents of spectroscopic
grade obtained from Sigma-Aldrich: pentane (Pent), n-hexane, cyclohexane, methylcyclohexane, 3-methylpentane, toluene
(Tol), ethyl acetate (EtAc), acetone, acetonitrile, and dimethyl sulfoxide
(DMSO). Spectral and photophysical measurements were also carried
out in DMSO and water (W) mixtures. The host–guest doping materials
were prepared by the melt-casting method. The host and guest materials
mixed according to the desired molar ratio were placed in an oxygen-free
sealed and clean reaction tube and then heated to melt. The temperature
was determined by the melting point of the host compounds triphenylphosphine
(TPP, 81.7 °C) or benzophenone (BPO, 48.5 °C).^{30 (link)} Dulbecco’s modified Eagle’s medium
(DMEM), fetal bovine serum (FBS), Trypsin, and penicillin/streptomycin
were purchased from Euroclone (Pero, Italy). Trypan Blue powder and
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
were purchased from Sigma-Aldrich (Saint Louis, MO) and Becton, Dickinson
and Company (Franklin Lakes, NJ).