Co ip assay kit 14311d

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Co-IP assay kit (14311D) is a laboratory tool designed to facilitate the study of protein-protein interactions. It provides the necessary reagents and protocols to perform co-immunoprecipitation experiments, allowing researchers to identify and analyze the formation of protein complexes within a sample.

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Co-IP kit (63 citations) IP/Co-IP Kit (9 citations) Co-IP assay kit (4 citations) Co-IP assay (2 citations)

4 protocols using co ip assay kit 14311d

Ubiquitin-mediated protein regulation assay

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Spautin-1 (S7888), Imatinib (S1026), and MG132 (S2619) were from Sellectchem (Houston, TX, USA). SKP2-C25 (M60136) was from Xcessbio Biosciences, Inc. (San Diego, CA). MTS reagent (G3582) was from Promega Corporation (Madison, WI, USA). Co-IP assay kit (14311D) was from Life Technologies (Carlsbad, CA). Antibodies: anti-Ubiquitin (#3936), anti-K63-Ubiquitin (#12930), anti-K48-Ubiquitin (#12805), anti-USP10 (#8501), anti-USP1 (#8033), anti-USP2 (#8036), anti-USP7 (#4833), anti-USP8 (#11832), anti-USP14 (#11931), anti-USP15 (#66310), anti-USP18 (#4813), anti-UCHL1 (#13179), anti-UCHL3 (#8141), anti-CYLD (#8462), anti-A20 (#5630), anti-SKP2 (#2652), anti-p27 (#3686), anti-FLAG (#14793), anti-HA (#3724), anti-phospho-c-Abl(Y245) (#2861), anti-c-Abl (#2862), anti-phospho-STAT5 (#9359), anti-STAT5 (#25656), anti-phospho-Crkl (#3181) and anti-Crkl (#38710) (Cell Signaling Technology, Beverly, MA, USA); anti-UCHL5 (ab124931), anti-USP13 (ab109264) (Abcam, Cambridge, MA); anti-GAPDH (BS60630) (Bioworld Technology, Inc., Louis Park, MN, USA).

Liao Y., Liu N., Xia X., Guo Z., Li Y., Jiang L., Zhou R., Tang D., Huang H, & Liu J. (2019). USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia. Cell Discovery, 5, 24.

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Investigating HIF-1α Regulation Pathways

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2-ME (S1233) and MG132 (S2619) were provided by Selleck. Cycloheximide (CHX) was provided by MedChemExpress (MCE, USA). MTS (G111) was a product of Promega (USA). The Co-IP assay kit (14311D) was provided by Life Technologies (USA). The Annexin V-FITC/PI apoptosis detection kit (KGA107) was provided by Keygen (China). Anti-PARP (9532), anti-Bax (14796), anti-Ubiquitin (3936), and anti-K48-ub (12805) antibodies were manufactured by Cell Signaling (USA). Anti-HIF-1α (ab179483) and anti-IGFBP-1 (ab181141) were from Abcam (UK). Anti-Bcl-2 (26593) was from Proteintech (USA). Anti-GAPDH (MB001) was provided by Bioworld Technology (USA). Immunoblot was performed with the antibodies diluted at 1:1000; for Co-IP, anti-HIF-1α antibodies (ab1) were utilized at 1:50. Anti-Bax antibodies for immunohistochemistry (Cell signaling) were utilized at 1:50. The HIF-1α siRNA (sc-45919) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The adenovirus and plasmids were provided by Vigene Biosciences (China) and GenePharma (China), respectively.

Tang X., Jiang H., Lin P., Zhang Z., Chen M., Zhang Y., Mo J., Zhu Y., Liu N, & Chen X. (2021). Insulin-like growth factor binding protein-1 regulates HIF-1α degradation to inhibit apoptosis in hypoxic cardiomyocytes. Cell Death Discovery, 7, 242.

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Proteasome Inhibition and Apoptosis Assay

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IU1 (catalog no. sc-361215), USP14 (sc-76817), AR (sc-29204), and UCHL5 (c-76797) siRNAs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). b-AP15 (S4920), MG132 (S2619), and bortezomib (S1013) were purchased from Sellectchem (Houston, TX, USA). Aur (catalog no. BML-EI206-0100) was obtained from Enzo Life Sciences International, Inc. (Plymouth Meeting, PA). MTS (catalog no. G111) was from Promega Corporation (Madison, WI, USA). AnnexinV-FITC/PI apoptosis detection kits (KGA107) were purchased from Keygen Company (Nanjing, China). Co-IP assay kit (14311D) was obtained from Life Technologies (Carlsbad, CA). Antibodies were from the following corporations: anti-GAPDH (MB001), anti-CDK2 (BS1050), anti-GFP (BSAP0675M), anti-Bcl-2 (BS70205) (Bioworld Technology, Inc., Louis Park, MN, USA); anti-Ubiquitin (catalog no. 3936), anti-PARP (9532), anti-CDK4 (12790), anti-USP14 (11931), anti-Lamin B (13435), anti-HSP90 (4877), HA-tag (3724), anti-K48-ub (12805), anti-cyclin D1 (2922) (Cell Signaling Technology, Beverly, MA, USA); anti-AR (ab108341), anti-UCHL5 (ab124931) (Abcam, USA).

Liao Y., Xia X., Liu N., Cai J., Guo Z., Li Y., Jiang L., Dou Q.P., Tang D., Huang H, & Liu J. (2018). Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene, 37(14), 1896-1910.

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Regulation of Estrogen Receptor Signaling

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MG132 (S2619), tamoxifen (S1238) were obtained from Selleck (Houston, TX, USA). Control siRNA (sc-37007) and USP15 siRNA (sc-76819) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). USP15-1 and USP15-2 siRNA (S0222) were from genechem (Shanghai, China). Cell-Light^TM EdU Apollo 567 In Vitro Kit (C10310-1) was obtained from RiboBio (Guangzhou, China). Annexin V-FITC/PI apoptosis detection kit (KGA107) was obtained from Keygen Company (Nanjing, China). The antibodies purchased from Cell Signaling Technology (CST, MA, USA) are listed below: anti-p21 (2947), anti-USP15 (66310), anti-ubiquitin (3936), anti-estrogen receptor α (8644), anti-phospho-Rb (8516), anti-Rb (9313), anti-Cyclin D1 (2922)(55506), anti-CDK4 (12790), anti-p27 (3686), anti-GAPDH (5174), anti-K48-ub (12805), anti-K63-ub (12930), anti-DYKDDDDK-tag (14793), anti-Myc-tag (2276). Anti-ERα (ab32063) was from Abcam (USA). Anti-USP15 (67557-1-lg) was from proteintech (Chicago, USA). Lentivirus was purchased from VigeneBio (Shandong, China) and plasmids were from Genechem (Shanghai, China). Cycloheximide (CHX) and Estrogen (E8875) were received from Sigma-Adrich (Sigma-Adrich, Louis, MO). MTS (catalog no. G111) was purchased from Promega Corporation (Madison, WI, USA). Co-IP assay kit (14311D) was purchased from Life Technologies (Carlsbad, CA).

Xia X., Huang C., Liao Y., Liu Y., He J., Shao Z., Hu T., Yu C., Jiang L., Liu J, & Huang H. (2021). The deubiquitinating enzyme USP15 stabilizes ERα and promotes breast cancer progression. Cell Death & Disease, 12(4), 329.

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