Chloroform

All materials for microgel synthesis were acquired from Sigma-Aldrich (Munich, Germany) unless stated otherwise. N-isopropylacrylamide (NIPAM—monomer,), N,N’- methylenebisacrylamide (BIS—crosslinking agent), ammonium persulfate (APS—initiator) were used as received. The organic solutions 1-octanol, 1-buthanol, decane, and tetradecane were purchased from Acros organics (Geel, Belgium). Tetrahydrofuran and acetone were received from PanReac AppliChem (Darmstadt, Germany). Chloroform, dioxane, 2-propanol, t-butanol, ethanol, hexane, dimethyl sulfoxide, acetonitrile, and cyclohexane were received from Chimmed (Moscow, Russia). Mineral oil was purchased from VWR (Darmstadt, Germany). The fat-soluble dye Sudan III (analytical standard) was obtained from Sigma-Aldrich (Munich, Germany). Water was purified using a Millipore Milli-Q system.

β-Nicotinamide adenine dinucleotide reduced was purchased from Serva (Heidelberg, Germany). Chloroform from Chimmed (Moscow, Russia), methanol from Merck (Darmstadt, Germany), D₂O 99.9% from AstraChem (Saint-Petersburg, Russia) were used as received. H₂O was deionized using an Ultra Clear UV plus TM water system (SG water, Hamburg, Germany) to the quality of 17.8 MOhm. Chemicals for HPLC were purchased from Sigma-Aldrich (St.Louis, MO, USA) and Cryochrom (Saint-Petersburg, Russia).

Polyethyleneimine (PEI) (Mw = 25,000, Mn = 10,000), poly(ethylene glycol) diglycidyl ether (PEGDGE) (Mn = 500), methanol (≥99.6%) (Sigma-Aldrich, St. Louis, MO, USA), chloroform, and 1-butanol (chemical grade, Chimmed, Russia) were taken without further purification.