Il1r1 mice

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro, United States

The Il1r1−/− mice are genetically engineered mice with a deletion of the Il1r1 gene, which encodes the interleukin-1 receptor type I. These mice lack the functional interleukin-1 receptor and are commonly used in research to study the biological roles of interleukin-1 signaling.

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21 protocols using il1r1 mice

Mouse Models for IL-1R and IL-33R

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WT BL/6 mice, 6 to 8 weeks of age, were obtained from The Jackson Laboratory (Bangor, ME) or Taconic (Hudson, NY). Il1rl1−/− (ST2/IL-33R) mice were a kind gift from Dr. Andrew McKenzie, Cambridge University. Il1r1−/− mice were obtained from Jackson. All animals were housed in a specific pathogen-free, Association for Assessment and Accreditation of Laboratory Animal Care-certified facility and handled according to Public Health Service Office of Laboratory Animal Welfare policies after review by the UAB Institutional Animal Care and Use Committee.

Garth J.M., Reeder K.M., Godwin M.S., Mackel J.J., Dunaway C.W., Blackburn J.P, & Steele C. (2017). IL-33 signaling regulates innate IL-17A and IL-22 production via suppression of PGE2 during lung fungal infection. Journal of immunology (Baltimore, Md. : 1950), 199(6), 2140-2148.

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Generation and Characterization of Knockout Mice

Cited 1 time

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C57BL/6J and IL-1R1^−/− mice were obtained from Jackson Laboratory. The IL-36α^−/− and IL-36α^−/−/IL-1R1^−/− mice have previously been described by us [6 (link)]. Wild type (C57BL/6J) and IL-1R1^−/− mice for experiments involving IL-36α^−/− and IL-36α^−/−/IL-1R1^−/− mice were bred in-house. All procedures were approved by the Temple University Institutional Animal Care and Use Committee.

Alvarez P, & Jensen L.E. (2016). Imiquimod Treatment Causes Systemic Disease in Mice Resembling Generalized Pustular Psoriasis in an IL-1 and IL-36 Dependent Manner. Mediators of Inflammation, 2016, 6756138.

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Mice Strains for Airway Experiments

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BALB/c, C57BL/6, BALB/cByJ, Tlr4^Lp^s^-d/J (Tlr4-d, BALB/cByJ background), Rag1^−/− mice (BALB/c background), Il1r1^−/− mice (C57BL/6 background), and PAR2^−/− mice (C57BL/6 background) were purchased from the Jackson Laboratory (Bar Harbor, ME). ST2^−/− (Il1rl1^−/−) mice (BALB/c background), Il17rb^−/− mice (BALB/c background) and Il13^+/eGFP mice (BALB/c background) were provided by Dr. Andrew McKenzie (Medical Research Council Laboratory of Molecular Biology, Cambridge, UK). Tslpr^−/− mice (BALB/c background) were provided by Dr. Steven Ziegler (Benaroya Institute, Seattle, WA). Il5^+/venus mice (BALB/c background) were provided by Dr. Kiyoshi Takatsu (University of Toyama, Toyama, Japan). Nlrp3^−/− mice (C57BL/6 background) were provided by Dr. Jurg Tschopp (University of Lausanne, Switzerland). All knockout or transgenic mice were bred in the Mayo Clinic animal care facility, and female mice aged 6–10 weeks were used for studies. All animal experiments and handling procedures were approved by the Mayo Clinic Institutional Animal Care and Use Committee and performed according to their guidelines.
Normal human bronchial airway epithelial (NHBE) cells were purchased from Lonza (Allendale, NJ) and maintained in serum-free bronchial epithelial cell growth medium (Lonza). NHBE cells were used within three passages.

Hara K., Iijima K., Elias M.K., Seno S., Tojima I., Kobayashi T., Kephart G.M., Kurabayashi M, & Kita H. (2014). Airway uric acid is a sensor of inhaled protease allergens and initiates type 2 immune responses in respiratory mucosa. Journal of immunology (Baltimore, Md. : 1950), 192(9), 4032-4042.

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Genetically Engineered Mouse Models for Immunology Research

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C57BL/6 WT, MyD88^−/− and Il1r1^−/− mice on C57BL/6 background were purchased from the Jackson Laboratory. IL-23R KO mice were imported from Genetech. hCD2-Cre mice (Jackson Laboratory) were crossed with Raptor^fl/fl mice, Rictor^fl/fl mice (Jackson Laboratory) and Stat3^fl/fl mice (Ding et al., 2016 (link)) to generate CD2-cre;Raptor^fl/fl, CD2-cre;Rictor^fl/fl and CD2-cre;Stat3^fl/fl conditional KO (cKO) mice. Male and female mice were used in all experiments (6–12 weeks old) and were housed in specific pathogen free conditions. All experiments were in accordance with institutional guidelines and approved by the IACUC at the University of Louisville.

Cai Y., Xue F., Qin H., Chen X., Liu N., Fleming C., Hu X., Zhang H.G., Chen F., Zheng J, & Yan J. (2019). Differential Roles of the mTOR-STAT3 Signaling in Dermal γδ T Cell Effector Function in Skin Inflammation. Cell reports, 27(10), 3034-3048.e5.

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Aerosol Infection of IL1r1 Knockout Mice

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IL1r1^–/– mice were purchased from Jackson Laboratories (catalogue no. JAX 3018) and backcrossed to C57BL/6 control mice from Taconic Farms (Hudson, NY) for 11 generations. Male and female mice, 8 to 12 weeks of age, were infected via the aerosol route with M. tuberculosis H37Rv (100 to 200 CFU/mouse) as previously described (13 (link)) and sacrificed 27 days later. In short, mice were infected using a whole-body inhalation system (Glas-Col, Terre Haute, IN) exposing the mice to aerosolized M. tuberculosis. Lungs were perfusion fixed in 4% paraformaldehyde and 0.4% glutaraldehyde overnight. After fixation, tissues were transferred to storage buffer containing 0.5% paraformaldehyde. All animals were maintained in Association for Assessment and Accreditation of Laboratory Animal Care (AALAC)-accredited biosafety level 2 (BSL2) or BSL3 facilities at the National Institutes of Health (NIH), and experiments were performed in compliance with an animal study proposal approved by the National Institute of Allergy and Infectious Diseases Animal Care and Use Committee.

van der Niet S., van Zon M., de Punder K., Grootemaat A., Rutten S., Moorlag S.J., Houben D., van der Sar A.M., Bitter W., Brosch R., Hernandez Pando R., Pena M.T., Peters P.J., Reits E.A., Mayer-Barber K.D, & van der Wel N.N. (2021). IL-1R1-Dependent Signals Improve Control of Cytosolic Virulent Mycobacteria In Vivo. mSphere, 6(3), e00153-21.

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Candida albicans Infection Model Development

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Three strains of mice were used to develop the screening method and to validate the infection model of Candida albicans. Female 20-week-old IL-1 receptor (IL1r1^−/−) mice (The Jackson Laboratory, Bar Harbor, ME), C57Bl/6 wild-type and mouse β-defensin-1 (mBD-1)-deficient (DefB1^−/−) female mice (henceforth referred to as mBD-1^−/−), developed on a C57Bl/6 background [22 (link)] and bred at the University of Florida Animal Care Services facility, were used in the study. All mice were given autoclaved standard rodent diet and sterile water ad libitum, and acclimated for at least 7 days after transfer from The Jackson Laboratories or the transgenic breeding facility at the University of Florida. Some mice were housed in HEPA filter-covered microisolator cages in ventilated racks in the AAALAC accredited facilities at Case Western Reserve University School of Medicine under approved protocols from the Institutional Animal Care and Use Committee.

Ryan L.K., Hise A.G., Hossain C.M., Ruddick W., Parveen R., Freeman K.B., Weaver D.G., Narra H.P., Scott R.W, & Diamond G. (2020). A Novel Immunocompetent Mouse Model for Testing Antifungal Drugs Against Invasive Candida albicans Infection. Journal of Fungi, 6(4), 197.

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Transgenic Mouse Models for Research

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5–8 week-old male mice were used at the outset of all experiments. C57BL/6J mice were obtained from Jackson Laboratories. Il1r1^−/− mice (> 10 generations backcrossed to C57BL/6) were obtained from Jackson Laboratories. Nestin-GFP mice (> 10 generations backcrossed to C57BL/6) were obtained from G. Enikolopov (Cold Spring Harbor Laboratories). All experimental protocols were performed in compliance with the Washington University School of Medicine Animal Safety Committee (protocol# 20140122).

Garber C., Vasek M.J., Vollmer L.L., Sun T., Jiang X, & Klein R.S. (2018). Astrocytes decrease adult neurogenesis during virus-induced memory dysfunction via interleukin-1. Nature immunology, 19(2), 151-161.

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Transgenic Mouse Models for Research

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Genetically Modified Mouse Models for Immunological Studies

Cited 2 times

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Balb/c, C57BL/6, Rag1^−/− and Il1r1^−/− mice were purchased from Jackson Laboratory (Bar Harbor, ME). ST2^−/− (Il1rl1^−/−) (18 (link)), Il17rb^−/− (19 (link)), Tslpr^−/− (20 (link)), and Il13^+/eGFP mice (19 (link)) were generated on the Balb/c background and have been bred in the animal facility at the Mayo Clinic (Rochester, MN). Il5^+/venus mice (21 (link)) were a gift from Dr. Kiyoshi Takatsu (University of Toyama, Toyama, Japan). Six- to 12-week-old female mice were used in this study. All the experiments in this study were approved by the Mayo Clinic Institutional Animal Care and Use Committee.

Iijima K., Kobayashi T., Hara K., Kephart G.M., Ziegler S.F., McKenzie A.N, & Kita H. (2014). IL-33 and Thymic Stromal Lymphopoietin Mediate Immune Pathology in Response to Chronic Airborne Allergen Exposure. Journal of immunology (Baltimore, Md. : 1950), 193(4), 1549-1559.

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Murine Models for Inflammatory Studies

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Female and male C57, CCR2^−/− and IL-1R1^−/− mice, 6–8 weeks of age, were obtained from Jackson Laboratory (Bar Harbor, ME). IL-1β^−/− mice were bred in-house as a result of a gift from Dr. David Caplin, University of Alabama. Five animals per cage were housed in microisolator units under pathogen-free conditions and were acclimated for one week prior to experimentation after arrival. Animals were fed a standard laboratory diet and water ad libitum. The University Committee on Animal Resources approved all animal protocols.

Groves A.M., Johnston C.J., Williams J.P, & Finkelstein J.N. (2018). Role of Infiltrating Monocytes in the Development of Radiation-Induced Pulmonary Fibrosis. Radiation research, 189(3), 300-311.

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