Human hepatocellular liver carcinoma (HepG2) cell line and human neuroblastoma cell line, SH-SY5Y, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained at 37 °C in a humidified atmosphere (95% air and 5% carbon dioxide), and they were periodically screened for contamination. HepG2 cells were cultured in Eagle’s Minimum Essential Medium (MEM, Euroclone S.p.A., Pero, MI, Italy), supplemented with 10% Fetal Bovine Serum (FBS, Euroclone S.p.A., Pero, MI, Italy), 1% L-glutamine (Euroclone S.p.A., Pero, MI, Italy), 100 U/mL penicillin/streptomycin (Euroclone S.p.A., Pero, MI, Italy), 1% Non-Essential Amino Acids (NEAA, Euroclone S.p.A., Pero, MI, Italy). SH-SY5Y cells were grown in a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM)—high glucose (Euroclone S.p.A., Pero, MI, Italy) and Ham’s F12 Medium (Euroclone S.p.A., Pero, MI, Italy) supplemented with 10% FBS, 1% L-glutamine and 100 U/mL penicillin/streptomycin. For cell assays, cells were trypsinized using Trypsin-EDTA 1X in PBS (Euroclone S.p.A., Pero, MI, Italy).
Sh sy5y
Manufactured by Euroclone
Sourced in United States, Italy
SH-SY5Y is a human-derived cell line commonly used in neuroscience research. It is a subclone of the SK-N-SH neuroblastoma cell line. SH-SY5Y cells exhibit neuronal characteristics and are suitable for studying various aspects of neuronal function and development.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Euroclone (4 mentions)
Penicillin streptomycin, by Euroclone (3 mentions)
Sh sy5y, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Euroclone (2 mentions)
Non essential amino acids neaa, by Euroclone (1 mentions)
Ham s f12 medium, by Euroclone (1 mentions)
Trypsin edta 1x, by Euroclone (1 mentions)
Minimum essential medium (mem), by Euroclone (1 mentions)
Dmem high glucose, by Euroclone (1 mentions)
Hek293t, by Euroclone (1 mentions)
Dulbecco modified eagle medium (dmem), by Euroclone (1 mentions)
Bovine serum, by Euroclone (1 mentions)
Sh sy5y human neuroblastoma cells, by Merck Group (1 mentions)
Penicillin and streptomycin, by Euroclone (1 mentions)
Pcr single locus technology, by Eurofins (1 mentions)
Rpmi 1640 medium, by Euroclone (1 mentions)
Imr 32, by American Type Culture Collection (1 mentions)
Sk n as, by American Type Culture Collection (1 mentions)
Sk n be 2 c, by American Type Culture Collection (1 mentions)
4 protocols using sh sy5y
1
Culturing Human Liver and Neuroblastoma Cells
2
Neuroblastoma and Kidney Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N-enriched SH-SY5Y (human neuroblastoma, ATCC®, Manassas, VA, USA) and HEK293T (human embryonic kidney, ATCC®) cell lines were grown and propagated in Dulbecco’s Modified Eagle’s Medium (DMEM, EuroClone®, Milan, Italy) supplemented with 2 mM L-glutamine (EuroClone®), a solution of 1% penicillin/streptomycin (EuroClone®), and 10% fetal bovine serum (FBS, EuroClone®). In particular, the N-enriched population of SH-SY5Y was obtained from the parental cell line by a procedure reported elsewhere [4 (link)].
3
SH-SY5Y Cell Culture and Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SH-SY5Y human neuroblastoma cells (Sigma-Aldrich, St. Louis, MO, USA, from The European Collection of Authenticated Cell Cultures, ECACC, Public Health England) were used. This cell line is not listed as a commonly misidentified cell line by the International Cell Line Authentication Committee (ICLAC; http://iclac.org/databases/cross-contaminations/ , on 16 January 2023) and was not further authenticated during the last five years. The SH-SY5Y cells were grown in Dulbecco’s Modified Eagle Medium plus F12 in a 1:1 ratio, containing 10% bovine serum, 1% L-glutamine, and 1% penicillin–streptomycin (all from Euroclone, Italy); they were maintained at 37 °C in a humidified 5% CO2 atmosphere and used within passage 30. For the Western blot (WB) and Co-IP experiments, the cells were seeded in six-well plates at a density of 500,000/4 mL/well. Under these conditions, the cells are not differentiated into neurons. Twenty-four hours after the onset of the culture, the cells were treated for 5 min with CGS 21680 (300 nM) or DHPG (10 µM). ZM 241385 (500 nM) and MPEP (10 µM) were applied 20 min before and then along with CGS 21680 or DHPG. The SH-SY5Y cells were maintained at 37 °C under 5% CO2 for the duration of the experiments.
4
Culturing and Characterizing Human NB Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SK-N-AS, SK-N-BE(2)C, SH-SY5Y, IMR-32 and HTLA-230 human NB-cell lines and human erythroleukemia cell line K-562 were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, USA). HTLA-230, IMR-32 and SK-N-AS were grown in Dulbecco’s Modified Eagle Medium high glucose (Euroclone, Milan, Italy). SK-N-BE(2)C, SH-SY5Y and K-562 were grown in Roswell Park Memorial Institute (RPMI) -1640 medium (Euroclone). Cell culture media were supplemented with 2 mM L-glutamine (Euroclone), 1% penicillin and streptomycin (Euroclone) and 10% fetal bovine serum (FBS; Thermo-Fisher Scientific, Waltham, USA). Primary bone marrow-derived mesenchymal stromal cells (BM-MSC) were isolated and cultured as previously described.23 (link) Cell lines were certified for their identity by PCR-single-locus-technology (Eurofins-Genomics, Ebersberg, Germany). Cultures were periodically tested to confirm the absence of Mycoplasma by Mycoplasma Detection Kit (Venor-GeM Advance, Berlin, Germany). The different NB cell lines were employed within a limited number of passages after their acquisition from ATCC: SHSY5Y (4–20), IMR32 (8–18), SKNAS (7–28), SKNBE2c (3–14) HTLA (4–12). During their period of utilization, the different NB cell lines maintained a stable phenotype. Their genetic status concerning MYCN, 1p36 and p53 amplification/mutation is summarized in online supplemental table 1 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!