4 μl of 1 μM assembled clathrin cages was applied to a glow-discharged formvar carbon 300-mesh copper grid (Agar Scientific) and allowed to adsorb for 1 minute. The sample was removed by blotting and 4 μl of 2% w/v uranyl acetate (UA) rapidly applied to the grid. This was incubated at room temperature for 1 minute to stain the sample after which the UA removed by blotting. Grids were air dried at room temperature before transfer to the microscope. Samples were imaged using a JEOL 2010F FEG transmission electron microscope with a Gatan Ultrascan 2000™ camera, at an accelerating voltage of 200kV.
Ultrascan 2000 camera
Manufactured by Ametek
The Ultrascan 2000™ camera is a high-performance imaging device designed for laboratory and scientific applications. It features a state-of-the-art sensor and advanced imaging capabilities to capture detailed, high-quality images. The Ultrascan 2000™ is capable of delivering precise and reliable data for a variety of analytical and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ultrascan 2000 camera
1
Visualizing Clathrin Caged Nanostructures
2
Visualizing Clathrin Caged Nanostructures
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