Bone marrow isolated F4/80+ macrophages (BMMs) were seeded onto coverslips and fixed with 4% PFA pH 7.4 for 40 min on ice, then washed twice with DPBS. Quenching was done using 0.1 M ethanolamine for 5 min and room temperature, twice. Coverslips were washed twice with DPBS and incubated in a buffer containing DPBS, 3% BSA, 0.4% saponin for 20 min at room temperature. Coverslips were then incubated in a buffer containing DPBS, 1% BSA and 0.15% saponin for 10 min before incubation with the LAMP1 primary antibody (Developmental Studies Hybridoma Bank, cat. number 1D4B, 1:100 dilution) for 1 hr at room temperature. Cells were then washed three times with DPBS and incubated with the secondary antibody in the same buffer (Invitrogen, cat. number A-2121, 1:2000 dilution) for 1 hr at room temperature. Coverslips were then washed three times with DPBS and stained with DAPI (1:30000 dilution) for 1 min before mounting with pro-long antifade (Thermofisher, cat. number P36930). Images were taken using the DeltaVision Elite Deconvolution microscope.
Lamp1 primary antibody
Manufactured by Developmental Studies Hybridoma Bank
The LAMP1 primary antibody is a laboratory reagent used in various research applications. It is a glycoprotein that serves as a marker for lysosomal membranes. The antibody can be utilized in techniques such as immunohistochemistry and Western blotting to detect and analyze the presence and distribution of LAMP1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lamp1 primary antibody
1
Immunofluorescent Labeling of Macrophage LAMP1
2
Immunofluorescent Labeling of Macrophage LAMP1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bone marrow isolated F4/80+ macrophages (BMMs) were seeded onto coverslips and fixed with 4% PFA pH 7.4 for 40 min on ice, then washed twice with DPBS. Quenching was done using 0.1 M ethanolamine for 5 min and room temperature, twice. Coverslips were washed twice with DPBS and incubated in a buffer containing DPBS, 3% BSA, 0.4% saponin for 20 min at room temperature. Coverslips were then incubated in a buffer containing DPBS, 1% BSA and 0.15% saponin for 10 min before incubation with the LAMP1 primary antibody (Developmental Studies Hybridoma Bank, cat. number 1D4B, 1:100 dilution) for 1 hr at room temperature. Cells were then washed three times with DPBS and incubated with the secondary antibody in the same buffer (Invitrogen, cat. number A-2121, 1:2000 dilution) for 1 hr at room temperature. Coverslips were then washed three times with DPBS and stained with DAPI (1:30000 dilution) for 1 min before mounting with pro-long antifade (Thermofisher, cat. number P36930). Images were taken using the DeltaVision Elite Deconvolution microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!