Cells were plated at 100,000 cells per well in a 24-well plate in 10% FCS medium until they reach 80% of confluence. Then, cells were washed once with sterile PBS and medium was changed for 0% FCS medium before a scratch was performed in the cell layer using a 200 µL tip. Cells were incubated for 48 h. A total of three pictures per wound were taken using an EVOS FLC imaging system (Life Technologies) at ×10 magnification. The size of the wound was then measured on each picture. Graphs show an average of three experiments. The wound healing assay could not be performed using G166 or GS090, as previously reported61 (link).
Evos flc imaging system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The EVOS FLc Imaging System is a compact, easy-to-use fluorescence microscope designed for routine cell imaging and analysis. It features a high-quality optical system and LED illumination, providing clear, detailed images of fluorescently labeled cells and samples.
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Lab products found in correlation
5 protocols using evos flc imaging system
1
Scratch Wound Healing Assay
2
Calcein AM Cell Viability Assay
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To further establish the effect of inhibitor dosages on cell viability, we performed a Calcein AM assay. Cells were seeded (5 × 105 cells/well) in confocal dishes and incubated for 6, 12, 24, and 48 h at 37 °C and 5% CO2 humidified atmospheric conditions. Spent media was removed from cells and washed with PBS. This was followed by the addition of 500 μL Calcein AM prepared solution (8 mL PBS: 4 μL Calcein AM (reconstituted in 1 mmol/L, DMSO) to each well and incubated in the dark at 37 °C for 30 min. Calcein AM solution was removed and washed with PBS. We added 500 μL of PBS to the cells before observing and images were captured using the GFP channel on the fluorescence microscope (Life Technologies EVOS FLc Imaging System, Carlsbad, CA USA).
3
Fluorescent Imaging of Flat Mounts and Cells
4
Immunohistochemical Analysis of SHP-1 and F4/80
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Paraffin-embedded AT sections (6 μm) were immunostained with monoclonal anti-SHP-1 (ab32559, 1:100) and anti-F4/80 antibody (MAB5580, 10 μg/mL) for immunohistochemical analysis. The bright-field and fluorescence images (GFP filter) of the stained sections were captured by EVOS FLc imaging system (Thermo Fisher Scientific, USA).
5
Standardized Fluorescence Imaging Protocol
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All images of stained slides were taken under same magnification (40X) and exposure time using EVOS FLc Imaging System (Thermo Fisher Scientific, Waltham, MA).
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