Dmem medium

Manufactured by HiMedia

Sourced in India

DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium. It provides essential nutrients and growth factors required for the in vitro maintenance and proliferation of various cell types.

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Lab products found in correlation

Sodium bicarbonate, by HiMedia (2 mentions) Fetal bovine serum (fbs), by HiMedia (2 mentions) Mcf 7, by HiMedia (1 mentions) Mda mb 231, by HiMedia (1 mentions) L 15 medium, by HiMedia (1 mentions) L glutamine penicillin streptomycin, by HiMedia (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 2 4 di tert butylphenol, by Merck Group (1 mentions) Propidium iodide, by HiMedia (1 mentions) Antibiotic solution, by HiMedia (1 mentions) Penicillin streptomycin p s, by Merck Group (1 mentions) Trisodium citrate dehydrate, by Merck Group (1 mentions) Ammonium hydroxide, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Tetraethyl orthosilicate, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Potassium carbonate, by Merck Group (1 mentions) Diacerein, by Merck Group (1 mentions) Huvec cells, by HiMedia (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

8 protocols using dmem medium

Evaluating BplzC's Effect on Cell Migration

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To evaluate the effect of BplzC on cell migration, an in vitro scratch assay was performed according to (Behera et al. 2020 (link)). HEK293 cells were cultured in six-well plates until they reached confluency. A straight scratch wound was created in the monolayer using a 10 µL pipette tip, and the cells were washed with sterile PBS to remove any debris. The wounded cell monolayer was treated with various concentrations of BplzC (0 ng, 5 ng, 10 ng, 20 ng, 40 ng, and 50 ng) incorporated in DMEM medium (HiMedia, # AT068), while cells treated with only DMEM medium served as the control. The cells were allowed to migrate for 12 or 24 h. The area of cell migration was calculated using Image J software, and the percentage of gap closure was determined using the following equation:
% of gap closure = Initial scratch area-Final scratch area / Initial scratch area × 100

Ojha P., Kar N.P., Behera H.T., Parija M., Nayak S., Singh S., Patra A.K, & Sahoo K.K. (2023). Independent antioxidant and anticancer properties of a novel thermostable lysozyme isolated from Bacillus paralicheniformis: in silico and in vitro studies. 3 Biotech, 13(7).

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Culturing HT29 Colon Cancer Cells

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HT29 cells were procured
from the National Centre for cell science, Pune, India, and grown
in DMEM medium (Hi-media, India) supplemented with 4.5 g of glucose
per liter, l-glutamine, 3.7 g per liter of sodium bicarbonate,
and 1 mM sodium pyruvate supplemented with 10% heat-inactivated fetal
bovine serum (FBS) 100 U/mL penicillin and 100 μg/mL streptomycin
at 37 °C under a humidified atmosphere of 5% CO and 95% air.
For periodical maintenance of cells, 1 × 10⁷ cells
were routinely maintained in culture and experimental vessels.

Cholayil Palapetta S., Gurusamy H, & Ganapasam S. (2023). Synthesis, Characterization, Computational Studies, Molecular Docking, and In Vitro Anticancer Activity of Dihydropyrano[3,2-c]chromene and 2-Aminobenzochromene Derivatives. ACS Omega, 8(8), 7415-7429.

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Evaluating Cytotoxicity of P. sophore Mucus

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Human normal colon cells (CRL-1831) were cultured in DMEM medium (Hi-Media^®, Mumbai, India) supplemented with 5% FBS, 1% penicillin-streptomycin at 37 °C in a humidified atmosphere of 5% CO₂/95% air. The culture medium was replaced every 2–3 days. Cytotoxic effect of P. sophore mucus extract was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Cells were seeded in 96-well plates at a density of more than 1 × 10⁵ cells per well and incubated in humidified atmosphere containing 5% CO₂ at 37 °C up to adherence. Cells were then treated with different concentrations of P. sophore mucus extract (20–100 μg/mL) for 48 h. After incubation, cells were washed with PBS solution and subjected to 100 μL of MTT solution (5 mg/mL) and further incubated for 4 h. Finally, the medium was removed and 100 μL of dimethyl sulfoxide (DMSO) was added to solubilize the formazan crystals. Amount of formazan crystal was determined by measuring the absorbance at 570 nm using enzyme-linked immunosorbent assay (ELISA) reader. Assays were done in triplicate and viability was expressed in % of control.

Patel M., Ashraf M.S., Siddiqui A.J., Ashraf S.A., Sachidanandan M., Snoussi M., Adnan M, & Hadi S. (2020). Profiling and Role of Bioactive Molecules from Puntius sophore (Freshwater/Brackish Fish) Skin Mucus with Its Potent Antibacterial, Antiadhesion, and Antibiofilm Activities. Biomolecules, 10(6), 920.

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Culturing Breast Cancer Cell Lines

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Human breast cancer cells MDA-MB-231, MCF-7, T47D and ZR-75-1 were obtained from National cell repository facility of India - National Center for Cell Science, Pune, India, which were originally procured from American Type Culture Collection, USA. Cells once procured, were kept in cultures and used within 1–2 months for the different experiments. MDA-MB-231 cells were grown in L-15 medium (Himedia, India) supplemented with L-Glutamine. MCF-7 cells were grown in DMEM medium (Himedia, India). T47D and ZR-75-1 cells were grown in RPMI-1640. All the media in which the cells were grown contained 10% FBS (GIBCO) and 1% L-Glutamine-Penicillin-Streptomycin (200 mM L-Glutamine, 10,000 units/mL Penicillin and 10 mg/mL Streptomycin) (Himedia, India). The MCF-7, T47D and ZR-75-1 cells were maintained at 37 °C in a humidified incubator with CO₂ while MDA-MB-231 cells were maintained at 37 °C in a humidified incubator without CO₂.

Moirangthem A., Bondhopadhyay B., Mukherjee M., Bandyopadhyay A., Mukherjee N., Konar K., Bhattacharya S, & Basu A. (2016). Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes. Scientific Reports, 6, 21903.

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Metallohydrogel Synthesis and Characterization

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2,4-Di-tert-butylphenol was purchased from Sigma-Aldrich. Manganese acetate, aspartic acid and indomethacin were obtained from alfa SRL. Gemcitabine was bought from BLD pharm. Commercially available forms of formaldehyde, ethanol, and methanol was employed without additional purification. Milli Q water was used for the formation of metallohydrogel. Other common solvents of the analytical reagent grade were utilised. DMEM medium, sodium bicarbonate, antibiotic solution and FBS, propidium iodide and calcein AM were purchased from Himedia, Sigma-Aldrich, and invitrogen.

Dutta M., Banerjee S., Mandal M, & Bhattacharjee M. (2023). A self-healable metallohydrogel for drug encapsulations and drug release. RSC Advances, 13(23), 15448-15456.

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Synthesis of Doxorubicin-Loaded Silica Nanoparticles

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Tetraethylorthosilicate (TEOS, 98%) and ammonium hydroxide (NH₄OH, 28–30% in water), penicillin/streptomycin (P/S), and FBS were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol, hydrochloric acid (HCl, 36.5%), trisodium citrate dehydrate (Na₃C₆H₅O₇.2H₂O, 99%), potassium carbonate (K₂CO₃, 99%), and tris(hydroxymethyl)aminomethane (C₄H₁₁NO₃ 99%) were purchased from Merck (Kenilworth, NJ, USA). Doxorubicin hydrochloride solution (2 mg/mL) was obtained from Ebewe Pharma (Unterach am Attersee, Austria). The DMEM medium was purchased from HIMEDIA (Mumbai, India). Deionized water was used in all experiments.

Nguyen T.N., Nguyen T.T., Nghiem T.H., Nguyen D.T., Tran T.T., Vu D., Nguyen T.B., Nguyen T.M., Nguyen V.T, & Nguyen M.H. (2021). Optical Properties of Doxorubicin Hydrochloride Load and Release on Silica Nanoparticle Platform. Molecules, 26(13), 3968.

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Cell Culture Reagents and Cell Lines

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For cell culture, DMEM medium, Sodium bicarbonate, antibiotics, and others were obtained from standard manufactures (Invitrogen, Sigma and Himedia). Various antibodies were purchased from Cell Signaling Technology, Beverly, MA, USA and Santa Cruz Biotechnology, USA. IL-6R siRNA was purchased from Santa Cruz Biotechnology, USA. 5-Azacytidin (5-Aza) and Diacerein (Dia) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Other chemicals were purchased from Sigma-Aldrich, St. Louis, MO, USA and Himedia India. MDA-MB-468 and MDA-MB-231 cells were purchased from National Center for Cell Science, Pune, India. HUVEC cells were purchased from Himedia India.

Bharti R., Dey G., Das A.K, & Mandal M. (2018). Differential expression of IL-6/IL-6R and MAO-A regulates invasion/angiogenesis in breast cancer. British Journal of Cancer, 118(11), 1442-1452.

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Transfection of HepG2 and HeLa Cells

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Human hepatocellular carcinoma cell lines (HepG2) and human cervical cancer cell lines (HeLa) were obtained from National Center for Cell Science (NCCS), Pune, India and cultured in DMEM medium (Hi-Media, India) supplemented with 10% FBS (Hi-Media, India), glutamine (0.29 g/L), sodium bicarbonate (2.2 g/L), 100 µg ml -1 penicillin and streptomycin at 37 ºC in a humidified atmosphere with 5% CO2. Cell lines seeded at a density of 1×10 5 per well were transiently transfected with 2.0 µg of the luciferase reporter constructs per well at a confluency of 70-80% using Lipofectamine 2000 (Invitrogen, USA) as per the manufacturer's instructions.
After 48 h the cells were washed with PBS, lysed with lysis buffer and harvested to check the luciferase activity.

Karthi S., Rajeshwari M., Francis A., Saravanan M., Varalakshmi P., Houlden H., Thangaraj K, & Ashokkumar B. (2017). 3'-UTR SNP rs2229611 in G6PC1 affects mRNA stability, expression and Glycogen Storage Disease type-Ia risk. Clinica chimica acta; international journal of clinical chemistry, 471.

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