In each case CLL cells were analyzed for CD38 antigen and ZAP-70 protein expression (as described previously [21 (link)]). Fresh PB samples were stained with the following set of monoclonal antibodies: FITC mouse anti-human CD19 (Clone SJ25C1, Cat No.: 340409), PE-Cy5 mouse anti-human CD5 (Clone UCHT2, Cat No.: 555354) and CD38 PE (Clone HIT2, Cat No.: 555460) or anti-ZAP-70 PE (Clone Clone 1E7.2, Cat No.: 344636) (BD Biosciences, Franklin Lakes, NJ, USA). Cells were considered positive for ZAP-70 with a cut-off point of ≥20%, and positive for CD38 expression with a cut-off point ≥30%.
Pe cy5 mouse anti human cd5
Manufactured by BD
Sourced in United States
The PE-Cy5 mouse anti-human CD5 is a laboratory reagent used for the identification and analysis of CD5-expressing cells. It consists of a phycoerythrin-cyanine 5 (PE-Cy5) conjugated mouse monoclonal antibody that binds to the CD5 antigen on the surface of human cells.
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Lab products found in correlation
2 protocols using pe cy5 mouse anti human cd5
1
CLL Cell Phenotyping: CD38 and ZAP-70
2
CLL Immunophenotyping and FISH Analysis
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CLL cells were stained for CD38 antigen and ZAP-70 protein expression (as described previously [28] (link)). Fresh PB samples were stained with FITC mouse anti-human CD19 (Clone SJ25C1), PE-Cy5 mouse anti-human CD5 (Clone UCHT2) and CD38 FITC (Clone HIT2) or anti-ZAP-70 PE (Clone 1E7.2) (BD Biosciences). A cut-off point for ZAP-70 positivity in leukemic cells was ≥ 20%. Patients with CD38 expression lower or higher than 30% were classified as CD38 negative or positive, respectively.
I-FISH analysis. Detection of del(17p13.1) and del(11q22.3) was a part of the routine diagnostic practice. We used a previously described method [29] (link).
I-FISH analysis. Detection of del(17p13.1) and del(11q22.3) was a part of the routine diagnostic practice. We used a previously described method [29] (link).
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