The peptides PSELT (H-Phe-Gln-Ile-Cys-Val-Ser-Sec-Gly-Tyr-Arg-OH), [Ser46,49] PSELT [called inert PSELT (I-PSELT) as a control], and PSELT-dansyl were synthesized by Fmoc solid phase methodology on an automated peptide synthesizer (CEM, Saclay, France) as previously described [19 (link)]. KCl, NaCl, NaHCO3, CaCl2, MgSO4, KH2PO4, NaH2PO4, Na2HPO4, mannitol, glucose, Na-pyruvate, β-nicotinamide adenine dinucleotide (NADH), reduced disodium salt hydrate, 2,4 dinitrophenylhydrazine (DNPH), 2-thiobarbituric acid (TBA), bovine serum albumin (BSA), butylated hydroxyanisole, diethyl ether, ethylenediaminetetraacetic acid (disodium salt), diethylenetriamine pentaacetic acid, pyrogallol, streptomycin sulfate, tween-20, hydrogen peroxide (H2O2), and urea were purchased from Sigma Aldrich (St. Louis, MO, USA). Tunicamycin (TM) was from Cayman Chemical (Ann Arbor, MI, USA), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), penicillin/streptomycin and fetal bovine serum (FBS) were provided by Thermo Fisher Scientific (Waltham, MA, USA). All the solutions were freshly prepared before starting the experiments. Absolute ethanol, ethyl acetate, hydrochloric acid, methanol, and trichloroacetic acid (TCA) were from Carlo Erba Reagents (Cornaredo, Milano, Italy).
Trichloroacetic acid tca
Manufactured by Carlo Erba
Sourced in Italy, Germany, France
Trichloroacetic acid (TCA) is a chemical compound used in various laboratory applications. It is a colorless, crystalline solid with a pungent odor. TCA is a strong acid commonly used as a reagent in analytical and organic chemistry procedures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Sulfuric acid, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Urea, by Merck Group (2 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (2 mentions)
Reduced disodium salt hydrate, by Merck Group (1 mentions)
Streptomycin sulfate, by Merck Group (1 mentions)
Diethylenetriaminepentaacetic acid, by Merck Group (1 mentions)
Mannitol, by Merck Group (1 mentions)
Na pyruvate, by Merck Group (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
2 4 dinitrophenylhydrazine dnph, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide nadh, by Merck Group (1 mentions)
Tunicamycin, by Cayman Chemical (1 mentions)
Tween 20, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
3 protocols using trichloroacetic acid tca
1
Peptide Synthesis and Characterization
2
Antioxidant and Oxidative Stress Analysis
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Trolox C, ABTS (2, 2′-azinobis(3-ethylbenzothiazoline 6-sulfonate)), gallic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), IGEPAL CA-630, butylated hydroxytoluene (BHT), dithiothreitol (DTT), hemin, cumene hydroperoxide (CHP), hydrochloric acid (HCl), n-butanol, Na 2 CO 3 , Folin-Ciocalteau reagent, phosphotungstic acid (PTA), potassium persulfate, sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), sulfuric acid, thiobarbituric acid, Trizma base and urea were purchased from Merck-Fluka-Sigma-Aldrich (Darmstadt, Germany).
Ethanol, petroleum ether and trichloroacetic acid (TCA) were purchased from Carlo Erba Reagents S.r.l. (Cornaredo MI, Italy). Acetic acid glacial was purchased from AppliChem (Munich, Germany). Sodium tetraborate was purchased from VWR (Milano, Italy). 2,4-Dinitrophenylhydrazine (DNPH) was purchased from JT Baker (Thermo Fisher Scientific, Milano, Italy) .
Ethanol, petroleum ether and trichloroacetic acid (TCA) were purchased from Carlo Erba Reagents S.r.l. (Cornaredo MI, Italy). Acetic acid glacial was purchased from AppliChem (Munich, Germany). Sodium tetraborate was purchased from VWR (Milano, Italy). 2,4-Dinitrophenylhydrazine (DNPH) was purchased from JT Baker (Thermo Fisher Scientific, Milano, Italy) .
3
Iodine Analysis in Egg Yolk and White
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An iodine solution was prepared by dissolving potassium iodate (Carlo-Erba, France) in deionized water (Millipore, USA). Trichloroacetic acid (TCA) was purchased from Carlo-Erba, France. Sodium dodecylsulfate (SDS) was purchased from Sigma-Aldrich, USA. Ceric sulfate and arsenious oxide (A&M, USA) were used in the Sandell-Kolthoff method. Hydrochloric acid, sulfuric acid, acetic acid, and sodium hydroxide were purchased from Merck, USA. Ammonium sulfate and zinc sulfate heptahydrate (Carlo-Erba, France) and potassium carbonate (Ajax) were used for the alkali ashing method. Egg samples were purchased from a local supermarket and stored in a refrigerator. Five eggs were cracked and only yolk and white egg were homogenized and used as a composited egg sample.
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