For blood and tumor samples from mice, the following antibodies were purchased from BioLegend: anti-human CD45-APC (BioLegend Cat# 304011, RRID:AB_314399), anti-human CD45-Brilliant Violet 421™ (BioLegend Cat# 304032, RRID:AB_2561357), anti-human CD4-APC (BioLegend Cat# 300514, RRID:AB_314082), anti-human CD4-PE/Cyanine7 (BioLegend Cat# 300512, RRID:AB_314080), anti-human CD8-FITC (BioLegend Cat# 980908, RRID:AB_2888883), anti-human CD8-Brilliant Violet785™ (BioLegend Cat# 344739, RRID:AB_2566201), anti-mouse CD45-APC (BioLegend Cat# 103111, RRID:AB_312976), anti-mouse CD45-Brilliant Violet 711™ (BioLegend Cat# 103147, RRID:AB_2564383), anti-mouse/human CD11b-Brilliant Violet 570™ (BioLegend Cat# 101233, RRID:AB_10896949), anti-mouse/human CD11b-PE (BioLegend Cat# 101208, RRID:AB_312791), anti-mouse/human CD11b-PE/Cyanine7 (BioLegend Cat# 101215, RRID:AB_312798), anti-mouse Ly6G-FITC (BioLegend Cat# 127606, RRID:AB_1236494), anti-mouse Ly6G-PerCP/Cyanine5.5 (BioLegend Cat# 127616, RRID:AB_1877271), anti-mouse Ly-6C-PerCP/Cyanine5.5 (BioLegend Cat# 128012, RRID:AB_1659241), anti-mouse F4/80-PE/Cyanine7 (BioLegend Cat# 123114, RRID:AB_893478), anti-mouse F4/80-PE (BioLegend Cat# 123110, RRID:AB_893486).
Pe anti mouse human cd11b
Manufactured by BioLegend
Sourced in United States
The PE anti-mouse/human CD11b is a fluorochrome-conjugated antibody that binds to the CD11b antigen expressed on the surface of various cell types, including monocytes, macrophages, and granulocytes. It can be used in flow cytometry applications to identify and analyze these cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Apc anti mouse cd25, by BioLegend (2 mentions)
Fitc anti mouse cd4, by BioLegend (2 mentions)
Fitc anti human cd8, by BioLegend (1 mentions)
Apc anti human cd4, by BioLegend (1 mentions)
Pe cyanine7 anti human cd4, by BioLegend (1 mentions)
Anti mouse f4 80 pe cyanine 7, by BioLegend (1 mentions)
Anti mouse ly6g fitc, by BioLegend (1 mentions)
Pe anti mouse f4 80, by BioLegend (1 mentions)
Anti mouse cd45 brilliant violet 711, by BioLegend (1 mentions)
Apc anti mouse cd45, by BioLegend (1 mentions)
Apc anti human cd45, by BioLegend (1 mentions)
Zombie nir fixable viability kit, by BioLegend (1 mentions)
Pe anti mouse cd8a, by BioLegend (1 mentions)
Pe anti mouse foxp3, by BioLegend (1 mentions)
Pacific blue anti mouse cd3, by BioLegend (1 mentions)
Percp anti mouse cd8a, by BioLegend (1 mentions)
Apc cy7 anti mouse ly 6g ly 6c gr 1, by BioLegend (1 mentions)
Pe cy7 anti mouse cd62l, by BioLegend (1 mentions)
Apc anti mouse human cd44, by BioLegend (1 mentions)
Pe anti mouse rat human foxp3, by BioLegend (1 mentions)
7 protocols using pe anti mouse human cd11b
1
Multiparametric Flow Cytometry for Tumor Immune Profiling
2
Murine Immune Cell Phenotyping
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Single-cell suspensions were prepared from the spleens and peripheral blood of mice. Spleens were mechanically disrupted using the plunger end of a 10 ml syringe and resuspended in 1% FBS/PBS. Peripheral blood in centrifuge tubes was placed into a 4℃ high speed centrifuge and spun at 1500 rpm for 5 min. The supernatant was frozen at -20℃ for subsequent ELISA detection. Then, 2 ml of erythrocyte lysate was added to the precipitate of the centrifuge tube, vibrated and incubated at room temperature for 10 min. Then, the tubes were centrifuged, the supernatant was discarded, and the cells were resuspended in 1% FBS/PBS.
Zombie NIR Fixable Viability Kit (Biolegend, USA) were used to stain dead cells. For cell-surface markers, single-cell suspensions were harvested and incubated with anti-mouse/human CD11b-PE, anti-mouse Ly-6G/Ly-6C (Gr-1)-APC, anti-mouse CD4-FITC, anti-mouse CD8a-PE, anti-mouse CD25-APC, anti-mouse FOXP3-PE or appropriate isotype controls (Biolegend, USA) for 30 min at 4 °C. Fixation buffer was added for 20 min, then cells were resuspended in 1% PBS. Finally, all samples were analysed by flow cytometry, and data analysis was processed using FlowJo V10 (Treestar, Inc.).
Zombie NIR Fixable Viability Kit (Biolegend, USA) were used to stain dead cells. For cell-surface markers, single-cell suspensions were harvested and incubated with anti-mouse/human CD11b-PE, anti-mouse Ly-6G/Ly-6C (Gr-1)-APC, anti-mouse CD4-FITC, anti-mouse CD8a-PE, anti-mouse CD25-APC, anti-mouse FOXP3-PE or appropriate isotype controls (Biolegend, USA) for 30 min at 4 °C. Fixation buffer was added for 20 min, then cells were resuspended in 1% PBS. Finally, all samples were analysed by flow cytometry, and data analysis was processed using FlowJo V10 (Treestar, Inc.).
3
Comprehensive Immune Profiling of Murine Tumors
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Carprofen (Rimadyl® Injectable, 50 mg/mL) was purchased from Patterson Companies (Saint Paul, MN, USA). Pacific Blue™ anti-mouse CD3, FITC anti-mouse CD4, PerCP anti-mouse CD8a, PE anti-mouse/human CD11b, APC/Cy7 anti-mouse Ly-6G/Ly-6C (Gr-1), PE/Cy7 anti-mouse CD62L, APC anti-mouse/human CD44, APC anti-mouse CD25, Biolegend PE anti-mouse/rat/human FOXP3, PerCP anti-mouse CD11c, PE/Cy7 anti-mouse CD86, FITC anti-mouse I-A/I-E, and APC anti-mouse CD40 were purchased from Biolegend (San Diego, CA, USA). Mouse tumor dissociation kit (No. 130-096-730) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
4
Multicolor Immune Cell Profiling
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For Lymphatic endothelial cell and monocyte/macrophage staining, cells were stained for PE anti-mouse/human CD11b (Biolegend, #101208), Pacific Blue™ anti-mouse CD45 (Biolegend, #103126), APC anti-mouse F4/80 (Biolegend, #123116), Lyve-1 (Abcam, #ab14917), Ly6C-PE (Thermo Fisher, #12593282), Alexa Fluor™ 488 goat anti-rabbit IgG (Thermo Fisher Scientific, #A11034). Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0).
5
Comprehensive Immune Cell Profiling in Mice
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After blocking with 2% rat serum, cells were stained for APC-Cy7 rat anti-mouse CD45 (BD), BV421 hamster anti-mouse CD11c (BD), PerCP-Cy5.5 rat anti-mouse I-A/I-E (BD), PE-Cy7 rat anti-mouse Ly-6C (BD), BV510 rat anti-mouse Ly-6G (BD), APC rat anti-mouse F4/80 (BD), PE anti-mouse/human CD11b (Biolegend), and Fixable Viability Stain 700 (BD). The stained cells were analyzed using BD FACS Canto II and DIVA software.
6
PMA-Induced Monocyte Differentiation
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THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA, 100 ng/mL; P1585, Sigma, USA) for 48 h. CD14 monoclonal antibody (APC) (17-0149-41, eBioscience, USA) or PE anti-mouse/human CD11b (101207, BioLegend, USA) fluorescent antibody was added to the cell suspension and incubated for 30 min away from light. The proportions of CD14- and CD11b-positive cells were detected via flow cytometry (CytoFLEX, Beckman, USA).
7
Macrophage Depletion Attenuates CIPN
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Liposomal clodronate at 1.05 mg/mouse was administered i.p. on days 5 and 7 of bortezomib treatment, and nociceptive threshold was measured daily until day 8. To confirm the successful macrophage depletion, the spleen was isolated from the mice that were sacrificed by cervical dislocation following the measurement of nociceptive threshold on day 8 after the onset of bortezomib treatment and subjected to detection of macrophages by flow cytometry using FITC anti-mouse F4/80 (1:100; Biolegend, Inc., San Diego, CA, USA) and PE anti-mouse/human CD11b (1:200; Biolegend, Inc.), as described previously [23 (link)]. To check the effect of macrophage depletion on the established CIPN after bortezomib treatment, the mice received a single i.p. administration of liposomal clodronate at 1.05 mg/mouse on day 13 after the onset of i.p. bortezomib.
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