Acquity beh phenyl column

Manufactured by Waters Corporation

The Acquity BEH Phenyl column is a high-performance liquid chromatography (HPLC) column developed by Waters Corporation. The column features a bonded phenyl stationary phase that is useful for the separation of a variety of analytes, including aromatic compounds. The Acquity BEH Phenyl column is designed to provide efficient and reliable chromatographic separations in HPLC applications.

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Lab products found in correlation

Api 3200, by AB Sciex (1 mentions) Tsq altis triple quadrupole mass spectrometer, by Thermo Fisher Scientific (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions)

Close spelling variants of this product

ACQUITY UPLC BEH Phenyl column (20 citations) BEH phenyl column (9 citations) BEH Phenyl (5 citations)

4 protocols using acquity beh phenyl column

Quantitative UPLC-MS/MS Method for α-GalCer

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A quantitative method was developed on a UPLC system (Acquity, Waters) coupled to a triple quadrupole mass spectrometer (API 3200, SCIEX). The chromatographic analysis was performed on an Acquity BEH Phenyl column (Waters, 100 × 2.1 mm, 1.7 µm), eluted with a short gradient program from 95:5 MeOH/H₂O to 100% MeOH in 1 min followed by an isocratic elution at 100% MeOH for 4 min. Flow rate was set at 0.4 ml/min and column temperature at 40°C. α-GalCer eluted at a Rt of 1.59 min, IS at 1.1 min.
A calibration curve was prepared by using five calibration points of α-GalCer standard (STD) (62.5, 125, 250, 500, and 1,000 ng/ml) spiked with 200 ng/ml IS and plotted as area ratio of STD/IS response vs concentration. Two MRM transitions were monitored for both STD and IS for quantitative purposes and to confirm analytical identification. The most intense transitions for each compound (i.e., m/z 856.7 > 178.9 for STD and m/z 698.5 > 89.2 for IS) were used as analytical responses.

Sartorius R., D’Apice L., Barba P., Cipria D., Grauso L., Cutignano A, & De Berardinis P. (2018). Vectorized Delivery of Alpha-GalactosylCeramide and Tumor Antigen on Filamentous Bacteriophage fd Induces Protective Immunity by Enhancing Tumor-Specific T Cell Response. Frontiers in Immunology, 9, 1496.

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DEHP and MEHP Quantification in Mouse Samples

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DEHP and MEHP in mouse plasma and liver from the dose–response study were determined according to a method modified from Miao et al. (2018 (link)). Briefly,

0.5 milliliter

plasma samples (

lowercase italic n equals 6

; plasma samples with hemolysis were not used) or

0.5 gram

liver tissue samples (

lowercase italic n equals 5

; two mice in the same group were combined in a pooled sample) was homogenized in

5 milliliters

acetate buffer (

0.2 molar

) with

100 microliters

β -glucuronidase

(

greater than or equal to 14,000 units per milliliter

) in a

10 milliliters

Dounce homogenizer. After spiking with

50 microliters

of the isotope internal standards solution (

0.3 milligram per liter

) (Cat# ALR-138S-CN; AccuStandard), the mixture was shaken for 12 h at 37°C for the enzymatic hydrolysis of phthalate conjugates. The analytes were chromatographically resolved using an ACQUITY BEH Phenyl column (

21 by 100 millimeters

1.7 micrometers

; Waters) with a linear solvent gradient from mobile phase A (acetonitrile) to mobile phase B (0.02% formic acid) at a flow rate of

0.35 milliliter per minute

. The eluent was analyzed using a TSQ Altis triple quadrupole mass spectrometer (Thermo Scientific) equipped with an electrospray ionization (ESI) interface in the multiple reaction monitoring mode.

Xu M., Li Y., Wang X., Zhang Q., Wang L., Zhang X., Cui W., Han X., Ma N., Li H., Fang H., Tang S., Li J., Liu Z., Yang H, & Jia X. (2022). Role of Hepatocyte- and Macrophage-Specific in Hepatotoxicity Induced by Diethylhexyl Phthalate in Mice. Environmental Health Perspectives, 130(1), 017005.

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Quantitative Analysis of Cyclodextrin Production

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A 20 g/L soluble starch solution prepared in 50 mM Na₂HPO₄/NaH₂PO₄ (pH 6.0) was incubated with the enzyme sample (20 mg/L) at 40 °C and 220 rpm for 18 h. The reaction was quenched via incubation in a boiling water bath for 10 min. The reaction product was diluted 10 times, filtered through a 0.22-μm membrane, and analyzed using an evaporative light-scattering detector Alltech 3300 (1000254412; Waters Corporation).
The concentrations of α-, β-, and γ-CDs in the final sample were determined via UPLC using an Acquity BEH phenyl column (2.1 × 100 mm, 1.7 µm; Waters) and eluted with a methanol/water ratio of 1:99 at 0.3 mL/min.

Zhang R., Tang R., Bi J., Shen S., Wu Q., Chen Q, & Li Y. (2023). Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase. Molecules, 28(3), 1245.

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UPLC Analysis of Bioactive Compounds

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UPLC analysis was performed on a Waters Acquity UPLC™ system (Waters, Milford, MA, USA) equipped with binary solvent delivery pump, an autosampler and PAD, which is connected to Empower 2 Pro software (version year 2005:2008, Waters). All separations were carried out on a Waters Acquity BEH Phenyl column (2.1 × 50 mm, 1.7 μm particle size). A binary gradient elution system composed of 0.1% aqueous phosphoric acid (v/v, Solvent A) and acetonitrile (Solvent B) was applied as follows: 0-0.4 min, 5% B; 0.4-1.5 min, 5-8% B; 1.5-3 min, 8% B; 3-3.5 min, 8-12% B; 3.5-4.3 min, 12% B; 4.3-8 min, 12-21% B; 8-9 min, 21-22% B; 9-16.5 min, 22-73% B; 16.5-22 min, 73% B; the total flow rate was 0.2 mL/min and the column temperature was maintained at 48°C. PAD was set to scan from 190 to 400 nm, and 210 nm was used as detection wavelength for fingerprint analysis with the sample injection volume of 0.5 μL.

Liang X., Zhao C, & Su W. (2016). A Fast and Reliable UPLC-PAD Fingerprint Analysis of Chimonanthus salicifolius Combined with Chemometrics Methods. Journal of chromatographic science, 54(7).

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