Quechers kit

Manufactured by Agilent Technologies

Sourced in United States

The QuEChERS kits from Agilent Technologies are sample preparation kits designed for the extraction and cleanup of a wide range of analytes from various matrices. The kits contain pre-weighed extraction salts and sorbents to facilitate efficient sample preparation.

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Lab products found in correlation

Mgso4, by Agilent Technologies (1 mentions) Sodium chloride (nacl), by Agilent Technologies (1 mentions) Ultrafree mc centrifugal filter, by Merck Group (1 mentions) Ceramic homogenizer, by Agilent Technologies (1 mentions) Whatman no 4 filter paper, by Cytiva (1 mentions) Ketoprofen d3, by Merck Group (1 mentions) Warfarin, by Merck Group (1 mentions) Centrifuge 5810r, by Eppendorf (1 mentions) Glacial acetic acid, by BD (1 mentions) N dodecane, by Merck Group (1 mentions) Fenpropimorph, by Merck Group (1 mentions)

7 protocols using quechers kit

HPLC-based Analytical Procedure

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HPLC grade reagents used for chromatography were obtained from Thermo Fisher Scientific (Waltham, MA, United States). All standard compounds used in the study were from Sigma-Aldrich Co. Ltd. (St. Louis, MO, United States). The QuEChERS kit used for extraction with a 50 ml tube with 4 g MgSO₄; 1 g NaCl; 1 g Na₃Citrate; 0.5 g Na₂HCitrate, purchased from Agilent (Santa Clara, CA, United States). Other commercial chemicals were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Jia Y., Liu Y., Hu W., Cai W., Zheng Z., Luo C, & Li D. (2023). Development of Candida autochthonous starter for cigar fermentation via dissecting the microbiome. Frontiers in Microbiology, 14, 1138877.

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Sample Preparation for Pesticide Metabolites

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Sample preparation of pesticide metabolites followed the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method with minor modifications.¹⁴ Briefly, a mixture of 1 mL urine sample, 10 µL internal standard chemicals diluted in acetonitrile, 4 mL ultrapure water, 10 mL acetonitrile, and the salt of the EN method QuEChERS kit (Agilent Technologies Japan, Ltd.) was vigorously shaken with a ceramic homogenizer and centrifuged at 3500 rpm (2356 g) for 10 minutes. The acetonitrile layer was transferred into a test tube and evaporated to dryness at 37°C under a stream of nitrogen. The dried residue was then dissolved in 200 µL ultrapure water, placed in a filter tube (Ultrafree‐MC Centrifugal Filter 0.2 µm pore size, hydrophilic PTFE, 0.4 mL volume, non‐sterile, Merck Ltd.), and centrifuged at 11 000 rpm (10 958 g) at 10°C for 3 minutes. The supernatant was transferred to a vial and analyzed using LC‐HRMS.

Nomasa K., Oya N., Ito Y., Terajima T., Nishino T., Mohanto N.C., Sato H., Tomizawa M, & Kamijima M. (2021). Development of a strategic approach for comprehensive detection of organophosphate pesticide metabolites in urine: Extrapolation of cadusafos and prothiofos metabolomics data of mice to humans. Journal of Occupational Health, 63(1), e12218.

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QuEChERS-Based Sample Extraction Protocol

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Sample preparation with a QuEChERS kit (containing 4 g of hydrous magnesium sulfate and 1 g of sodium chloride with ceramic homogenizer; Agilent Technologies, Palo Alto, CA) was conducted according to the Agilent application note and the method reported by Andrade et al., with some modifications [43 (link)]. The sample (2 g) was weighed into a 50 mL conical tube, water (10 mL) was added, the mixture was held for 5 min, and then 10 mL of ACN (2% formic acid, v/v) was added. The mixture was shaken for 20 min at 300 rpm, and then QuEChERS salt (4 g MgSO₄, 1 g NaCl) was added. Next, ceramic homogenizer was added, and the mixture was shaken manually for 3 min. The extract was then centrifuged at 5000× g for 5 min. The supernatant was filtered through Whatman No. 4 filter paper. The filtrate (5 mL) was dried under a stream of N₂ at 50 °C and then reconstituted with 50% MeOH.

Woo S.Y., Ryu S.Y., Tian F., Lee S.Y., Park S.B, & Chun H.S. (2019). Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup. Toxins, 11(10), 594.

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Pesticide Extraction and Analysis in Soil

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The pesticides belonging to different classes were extracted and clean-up by using the QuEChERS method (ILNAS-EN 15 662) [31] . All used chemicals were obtained from Sigma–Aldrich (USA). QuEChERS kits were purchased from Agilent Technologies (USA). Deionized water was supplied by the Millipore water purification system (Milli-Q). About 10 g of each soil sample was weighed into 50 ml centrifuge tube; 10 ml of acetic acid 1% was added and shaken for 2 min then ultrasonic for 1 min. Ten ml of acetonitrile was added to the tube and shaken vigorously for 2 min, then centrifuged for 5 min at 4000 rpm. An aliquot of 6 ml of the acetonitrile phase was moved into 10 ml centrifuge tube containing 1 g magnesium sulfate and 200 mg PSA, shaken energetically for 30 s and centrifuged (3000 rpm) for 2 min. The supernatant was evaporated to dryness then reconstituted in 2 ml acetone: n-hexane (1:9 v/v) then the mixture was subjected to ultra-sonication for 30 s then filtered into a glass vial. The extract is analyzed using LC-MS/MS and GC-MS/MS.

Ibrahim E.A, & Shalaby S.E. (2022). Screening and assessing of pesticide residues and their health risks in vegetable field soils from the Eastern Nile Delta, Egypt. Toxicology Reports, 9, 1281-1290.

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Analysis of Furocoumarin Content in Foods

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On the basis of previous work examining furocoumarin content of foods,^{2 ,25 (link),30 ,41 (link),45 (link)–48 (link)} seven furocoumarin standards were selected, based on their overall contribution to the total furocoumarin content of individual foods and total diets. Of these standards, six (bergaptol, psoralen, 8-MOP, bergapten, epoxybergamottin, and bergamottin) were purchased from Herboreal Ltd. (Edinburgh, U.K.) and 6′,7′-dihydroxybergamottin (6′,7′-DHB) was purchased from Cayman Chemical (Ann Arbor, MI). Warfarin, used as a surrogate, and ketoprofen-d3, used as an internal standard, were both purchased from Sigma-Aldrich (St. Louis, MO). QuEChERS kits were purchased from Agilent (Palo Alto, CA). A Mettler Toledo XS105 (Columbus, OH) analytical balance was used for the measurement of sample weights. A Fisher Scientific digital vortex mixer (Pittsburgh, PA) was used for sample mixing, and an Eppendorf 5810R centrifuge (Hauppauge, NY) was used for sample centrifugation.

Melough M.M., Lee S.G., Cho E., Kim K., Provatas A.A., Perkins C., Park M.K., Qureshi A, & Chun O.K. (2017). Identification and Quantitation of Furocoumarins in Popularly Consumed Foods in the U.S. Using QuEChERS Extraction Coupled with UPLC-MS/MS Analysis. Journal of agricultural and food chemistry, 65(24), 5049-5055.

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Pesticide Residue Analysis Protocol

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The reagents used in this study include: acetonitrile GC grade (Polskie Odczynniki Chemiczne, POCH, Gliwice, Poland), n-hexane pesticide residue analysis grade (POCH, Gliwice, Poland), ready-to-use QuEChERS kits (Agilent, Warsaw, Poland), glacial acetic acid (BDH, Poole, UK) and n-dodecane (Merck, Warsaw, Poland). The following certified standards were also: o,p’-DDE, p,p’-DDE, o,p’-DDD, p,p’-DDD, o,p’-DDT, p,p’-DDT, dieldrin and 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB 153) (Institute of Organic Industry, Warsaw, Poland) and heptachlor (Dr. Ehrenstorfer GmbH). All of the standards had a purity of >99%.

Lewiński R., Hernik A., Liszewska M., Buckley B., Czaja K., Korcz W., Słomczyńska A, & Struciński P. (2023). Validation of a Modified QuEChERS Method for the Determination of Selected Organochlorine Compounds in Honey. Molecules, 28(2), 842.

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Quantification of Fenpropimorph and Metabolite

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Analytical standards of fenpropimorph (96.2%) and fenpropimorph acid (99.7%) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and LGC Standards (Teddington, Middlesex, UK), respectively. All organic solvents used in this study were of HPLC grade, purchased from J.T Baker (Phillipsburg, NJ, USA). Other chemicals were of analytical grade, purchased from Junsel Chemical Co. (Chuo-ku, Tokyo, Japan). QuEChERS kits were obtained from Agilent Technologies (Santa Clara, CA, USA).

Kim S.W., Lim D.J, & Kim I.S. (2021). Simultaneous Analysis of Fenpropimorph and Fenpropimorph Acid in Six Different Livestock Products Using a Single-Sample Preparation Method Followed by Liquid Chromatography–Tandem Mass Spectrometry. Molecules, 26(19), 5791.

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