Abi veriti thermocycler

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ABI Veriti Thermocycler is a thermal cycler designed for DNA amplification. It features a block that can hold 96 samples in 0.2 mL tubes or a 96-well microplate. The instrument precisely controls the temperature and duration of each step in the PCR process to facilitate efficient DNA synthesis.

Automatically generated - may contain errors

Lab products found in correlation

Primescript rt reagent kit, by Takara Bio (2 mentions) Riboex reagent, by GeneAll (2 mentions) Taq dna polymerase master mix red, by Ampliqon (2 mentions) Iscript reverse transcription supermix, by Bio-Rad (1 mentions) Ssoadvance universal sybr green supermix, by Bio-Rad (1 mentions) Rneasy kit, by Qiagen (1 mentions) Cfx96 real time system, by Bio-Rad (1 mentions) Multiplex pcr kit, by Qiagen (1 mentions) Genemapper id v3, by Thermo Fisher Scientific (1 mentions) Abi prism 3130 genetic analyzer, by Thermo Fisher Scientific (1 mentions) Random hexamer, by Promega (1 mentions) Rnaeasy kit, by Qiagen (1 mentions) Superscript 2 rt, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Thermocycler (175 citations) Veriti (149 citations) Veriti thermocycler (99 citations) ABI Veriti (7 citations) ABI Veriti thermocycler platform (2 citations)

8 protocols using abi veriti thermocycler

RNA Extraction and RT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from the RNA later-stabilized tissues or cell line lysates using 1 ml RiboEx reagent (Gene All Biotechnology Co, South Korea).
cDNA was synthesized using PrimeScriptTM RT reagent Kit (TaKaRa, Japan). The reaction vessel was incubated in an ABI Veriti Thermocycler (Applied Biosystems). The control gene used in this study was human Beta-2-microglobulin (B2M). RT-PCR was performed with ABI Veriti Thermocycler (Applied Biosystems) using Taq DNA polymerase master mix red (Ampliqon, Copenhagen, Denmark). All PCR products were visualized by 1% agarose gel electrophoresis.GelQuant.NET was used to investigate the intensity of each band.

Soleymani Fard S., Sotoudeh M., Saliminejad K., Yazdanbod M., Mahmoodzadeh H., Kouchaki S., Yaghmaie M., Mousavi S.A., Malekzadeh R., Alimoghaddam K, & Ghaffari S.H. (2020). Investigation of the Correlation between Androgen Receptor and ZEB1 and its Value in Progression of Gastric Cancer. Avicenna Journal of Medical Biotechnology, 12(1), 52-60.

+ Open protocol

+ Expand

Real-Time qPCR Analysis of sFlt1, MX-1 and GAPDH

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated using RNeasy kit (QIAGEN) and 0.5 μg of RNA per condition was used for the cDNA synthesis using iScript Reverse Transcription Supermix (BIO-RAD), and an ABI Veriti Thermocycler (Life Technologies). SsoAdvance Universal SYBR Green Supermix and CFX96 Real Time System from BIO-RAD were used following manufacturer’s instructions for volumes and reaction settings. Primer sequences for sFlt1, MX-1 and GAPDH genes were previously described (28 (link), 33 (link), 34 (link)), and purchased from IDT Integrated DNA Technologies.

Andrade D., Kim M., Blanco L.P., Karumanchi S.A., Koo G.C., Redecha P., Kirou K., Alvarez A.M., Mulla M.J., Crow M.K., Abrahams V.M., Kaplan M.J, & Salmon J.E. (2015). Interferon-α and angiogenic dysregulation in pregnant lupus patients destined for preeclampsia. Arthritis & rheumatology (Hoboken, N.J.), 67(4), 977-987.

+ Open protocol

+ Expand

Ancestry Proportions Estimation Using AIMs

Check if the same lab product or an alternative is used in the 5 most similar protocols

A set of 61 Ancestry-Informative Markers (AIMs) was used to estimate the proportion of three different ancestries: European (EUR), African (AFR), and Amerindian (AMR). These indel-type markers were previously described [12 (link),19 (link)]. The AIMs were genotyped by Multiplex PCR, followed by capillary electrophoresis with fragment analysis. The multiplex amplifications were run using the QIAGEN Multiplex PCR kit (QIAGEN, Germany), with the PCR being run in an ABI Veriti thermocycler (Life Technologies, USA), followed by the capillary electrophoresis protocol. The DNA fragments were separated using an ABI PRISM 3130 Genetic Analyzer and peak reads were obtained in GeneMapper ID v3.2 software (Life Technologies). The experiment was replicated twice.

Leal D.F., Santana da Silva M.N., Fernandes D.C., Rodrigues J.C., Barros M.C., Pinto P.D., Pastana L.F., da Silva C.A., Fernandes M.R., de Assumpção P.P., dos Santos S.E, & dos Santos N.P. (2020). Amerindian genetic ancestry as a risk factor for tuberculosis in an amazonian population. PLoS ONE, 15(7), e0236033.

+ Open protocol

+ Expand

Sanger Sequencing of SLC25A46 Gene

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sanger sequencing was performed by standard methods on the 8 exons of the SLC25A46 gene (NM_138773.1). Primers were designed using Primer3. PCR products were amplified using 50ng DNA, with standard PCR reagents Econotaq (Lucigen, WI), on an ABI Veriti Thermocycler (Applied Biosystems, Austin, TX). PCR products were precipitated and sequencing PCR performed using BigDye Terminator Ready Reaction Mix (ABI Biosystems).

Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.

+ Open protocol

+ Expand

RNA Extraction and cDNA Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RiboEx reagent (GeneAll Biotechnology Co., South Korea) was used to extract total RNA from cell line lysates or the RNAlater-stabilized tissues. PrimeScriptTM RT reagent Kit (Takara, Japan) and an ABI Veriti Thermocycler (Applied Biosystems) were applied to synthesize complementary DNAs for 15 min at 37°C, and five seconds at 85°C.

Soleymani Fard S., Yazdanbod M., Sotoudeh M., Bashash D., Mahmoodzadeh H., Saliminejad K., Mousavi S.A., Ghaffari S.H, & Alimoghaddam K. (2020). Prognostic and Therapeutic Significance of Androgen Receptor in Patients with Gastric Cancer. OncoTargets and therapy, 13, 9821-9837.

+ Open protocol

+ Expand

RT-PCR and Agarose Gel Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Complementary DNAs (cDNAs) were amplified using specific primers. B2M (beta-2-microglobulin) was used as a control gene. RT-PCR was performed using Taq DNA polymerase master mix red (Ampliqon, Copenhagen, Denmark) with ABI Veriti Thermocycler (Applied Biosystems). One percent agarose gel electrophoresis was applied to visualize the PCR products.

+ Open protocol

+ Expand

RT-PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

For RT-PCR analysis of HeLa cells, total RNA was isolated using Qiagen RNA easy kit (Qiagen). The isolated RNA was treated with DNAse to avoid any DNA contamination. Equal amount of RNA was reverse transcribed into cDNA using superscript RT as described previously31 (link). cDNA of different samples was normalized using β-actin, and the specific products were amplified using specific primers (Table S1) on an ABI Veriti thermocycler (Applied Biosystems, USA).
SYBR green based real-time PCR was used to analyze the expression of specific genes in cerebellar granule neuron cultures (Table S1). ~1μg of total RNA was converted to cDNA using Random hexamers (Promega) and superscript RT-II (Invitrogen). The expression level of specific genes of interest was analyzed using Takara SYBR green mix with β-actin as internal control. The real-time analyzes were done by 2^−ΔΔCt method using SDS 2.1 software (ABI)35 (link).

Divya T.S., Lalitha S., Parvathy S., Subashini C., Sanalkumar R., Dhanesh S.B., Rasheed V.A., Divya M.S., Tole S, & James J. (2016). Regulation of Tlx3 by Pax6 is required for the restricted expression of Chrnα3 in Cerebellar Granule Neuron progenitors during development. Scientific Reports, 6, 30337.

+ Open protocol

+ Expand

Sanger Sequencing of SLC25A46 Gene

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!