L-Kynurenine sulfate salt, KYNA, and L-tyrosine disodium salt hydrate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). L-Alanine, L-arginine, L-asparagine, L-aspartate, L-cysteine hydrochloride monohydrate, L-glutamine, L-glutamate, L-glycine, L-histidine, L-isoleucine, L-leucine hydrochloride, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, and L-valine were purchased from Wako Pure Chemical Industries (Osaka, Japan). Rodent diet (MF) was obtained from Oriental Yeast Co., Ltd (Tokyo, Japan). All other chemicals were of the highest commercially available purity.
L asparagine
Manufactured by Fujifilm
Sourced in Japan
L-asparagine is a chemical compound that is commonly used as a lab reagent. It is an amino acid that plays a role in various metabolic processes. The core function of L-asparagine is to serve as a building block for proteins and other biomolecules in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
L alanine, by Fujifilm (3 mentions)
L glutamine, by Fujifilm (3 mentions)
L valine, by Fujifilm (2 mentions)
L methionine, by Fujifilm (2 mentions)
L serine, by Fujifilm (2 mentions)
L isoleucine, by Fujifilm (2 mentions)
L glycine, by Fujifilm (2 mentions)
L phenylalanine, by Fujifilm (2 mentions)
L proline, by Fujifilm (2 mentions)
L threonine, by Fujifilm (2 mentions)
L histidine, by Fujifilm (2 mentions)
L aspartic acid, by Fujifilm (2 mentions)
L glutamic acid, by Fujifilm (2 mentions)
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
L kynurenine sulfate salt, by Merck Group (1 mentions)
L tyrosine disodium salt hydrate, by Merck Group (1 mentions)
L arginine, by Fujifilm (1 mentions)
L cysteine hydrochloride monohydrate, by Fujifilm (1 mentions)
L glutamate, by Fujifilm (1 mentions)
4 protocols using l asparagine
1
Synthesis and Analysis of Amino Acids
2
Analytical-Grade Chemical Compounds
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Propionic acid (PA), butyric acid (BA), L-glycine (Gly), L-alanine (Ala), L-serine (Ser), L-threonine (Thr), L-cysteine (Cys), L-methionine (Met), L-valine (Val), L-leucine (Leu), L-isoleucine (Ile), L-phenylalanine (Phe), L-tryptophan (Trp), L-aspartic acid (Asp), L-glutamic acid (Glu), L-asparagine (Asn), L-glutamine (Glu), L-histidine (His), L-asparagine monohydrate (Arg), L-tyrosine (Tyr), and L-ascorbic acid were purchased from FUJIFILM Wako (Osaka, Japan). Acetic acid (AA), L-lysine (Lys), and sodium butyrate were purchased from Nacalai Tesque (Kyoto, Japan), and proline (Pro) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade.
3
Amino Acid Profiling Protocol
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A standard mixture of amino acids (type H), other amino acids, and imidazole peptides (such as L-hydroxyproline, L-asparagine, L-glutamine, β-Ala, L-Car, and L-Ans) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Bal was purchased from Hamari Chemicals (Osaka, Japan). Other reagents, including ammonium acetate, acetonitrile (HPLC grade), trifluoroacetic acid (TFA), and PITC, were purchased from FUJIFILM Wako Pure Chemical Corporation. Triethylamine was purchased from Thermo Fisher Scientific (Life Technologies, Carlsbad, CA, USA).
4
BV2 Cell Culture and Screening
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BV2 cells were cultured in either DMEM (high glucose; Wako) or DMEM/nutrient mixture F-12 (DMEM/F12; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS), 100 units penicillin and 100 mg streptomycin (P/S; Thermo Fisher Scientific). For screening, BV2 cells were cultured in DMEM with 10% FBS and P/S in the presence or absence of 150 μM L-alanine (Wako), 50 μM L-glutamic acid (Wako), 50 μM L-aspartic acid (Wako), 50 μM L-asparagine (Wako), 150 μM L-proline (Wako), 0.0052 pM CuSO4 (Wako), 1.5 pM FeSO4 (Wako), 1.5 pM ZnSO4 (Wako), 150 nM linoleic acid (Wako), 500 nM lipoic acid (Sigma), 15 nM biotin (Sigma), 500 nM vitamin B12 (Wako), and 15 μM hypoxanthine (Sigma). Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid tetrasodium salt (PPADS) was purchased from Alexis Corporation. Dipyridamole (DY) and allopurinol (ALP) were purchased from Tokyo Chemical Industry. Hoechst 33342 was purchased from Thermo Fisher Scientific. DAPI was purchased from Dojindo. FAT3 siRNAs (NM_001080814, siRNA ID; SASI_Mm02_00293873) and MISSION siRNA universal negative control (SIC001) were purchased from Sigma; 50 nM FAT3 siRNAs were transfected using Lipofectamine RNAi Max Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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