Ab206461

Antibodies and their suppliers were: MTH1 (1:1000, rabbit, NB100-109, NOVUS, Abingdon, Oxon, England, UK), OGG1 (1:500, rabbit, NB100-106, NOVUS), XRCC1 (1:1000, rabbit, A300-065A, Bethyl Laboratories, Montgomery, TX, USA), MUTYH (1:1000, rabbit, PA5-27855, ThermoFischer, Waltham, MA, USA), EBNA1 (1:100, rat, E1BS1H4-14111, supernatant), LMP1 (1:50, mouse, M0897, Dako, Glostrup, Denmark), BZLF1 (1:1000, mouse, SC-53904, Santa Cruz, Dallas, TX, USA), GAPDH (1:2000, mouse, CB1001, Merck Millipore, Darmstadt, Germany). Antibodies used for immunofluorescence: pH2AX (1:100, mouse, 05–636, Millipore Corporation, Billerica, MA, USA), Anti-8-oxodG (1:50, mouse, ab206461, Abcam, Cambridge, MA, USA). Chemicals and their suppliers were: TH588 (Sigma-Aldrich, SML1069, St Louis, MO, USA), (S)-Crizotinib (TOCRIS, 6025, Abingdon, Oxon, England, UK).

Alisol B was isolated from A. orientale by authors, and identified through ¹H and ¹³C NMR spectra. Recombinant human sEH was purchased from Cayman Chemical and used to detect the K_D value of alisol B with sEH. Hematoxylin and eosin (H&E) staining kit was obtained from Beyotime (Shanghai, China). The primary antibodies were purchased from Proteintech (Wuhan, China), Cell Signaling Technology (CST, Danvers, MA, USA), Abcam (Cambridge, United Kingdom), Boster (Wuhan, China) and Abclonal (Wuhan, China). Detailed information for the primary antibodies were as following: p53 (10442-1-AP, Proteintech), PARP (9542S, CST), Bcl-2 (3498S, CST), Bax (ab182734, Abcam), Caspase3 (ab49822, Abcam), Cleave-Caspase3 (9664S, CST), β-actin (BM0627, Boster), MCP-1 (66272-1-lg, Proteintech), ICAM-1 (60299-1-lg, Proteintech), iNOS (13120S, CST), IL-6 (21865-1-AP, Proteintech), p65 (8242T, CST), Grp78 (ab21685, abcam), 4-HNE (ab48506, Abcam), TNF-α (60291-1-lg, Proteintech), 8-OXO (ab206461, Abcam), HO-1 (A1346, ABclonal), Nrf2 (ab31163, Abcam), Keap1 (10503-2-AP, Proteintech), sEH (10833-1-AP, Proteintech), Kim-1 (14971S, CST), GSK3β (A2081, ABclonal), and p-GSK3β (5558P, CST).

After blocking in 3% BSA at 37°C for 1 h, the sections were overnight incubated at 4°C with the following antibodies: anti‐PXR (1:100, ab192579, Abcam, Cambridge, UK), anti‐4‐hydroxynonenal (4‐HNE, 1:50, ab46545, Abcam, Cambridge, UK), anti‐8‐oxo‐2′ ‐deoxyguanosine (8‐OXO, 1:50, ab206461, Abcam, Cambridge, UK), anti‐NLRP3 (1:200, BA3677, Boster, Wuhan, China), anti‐NeuN (1:500, MAB377, Millipore, MA, USA), anti‐GFAP (1:500, MAB360, Millipore, MA, USA), anti‐Iba‐1 (1:500, GB11105, Servicebio, Wuhan, China). They were subsequently washed with PBST and added with drops of appropriate fluorescence‐conjugated secondary antibodies (1:1000, Invitrogen, MA, USA) at room temperature for 1 h. The nuclei were stained with DAPI (Solarbio, Beijing, China) at room temperature for 20 min. Images were taken with a fluorescent microscope (Nikon, Tokyo, Japan). Three slices for each group were used for statistical analysis, and two fields were selected on each slice for abstaining signaling data.