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Bergamo 2 microscope

Manufactured by Thorlabs
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The Bergamo II microscope is a high-performance microscope designed for advanced imaging applications. It features a modular design, allowing for customization to suit various research and industrial needs. The Bergamo II provides exceptional optical quality and advanced imaging capabilities.

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Lab products found in correlation

Insight ds, by Spectra-Physics (12 mentions) H7422 40, by Hamamatsu Photonics (1 mentions) Ff02 525 40 25, by IDEX Corporation (1 mentions)

17 protocols using bergamo 2 microscope

1

Two-Photon Imaging of Neuronal Activity

Cited 1 time
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Two photon imaging was performed on a Bergamo II microscope (Thorlabs) running Scanimage (Pologruto et al., 2003 (link)) (Vidrio Technologies) with 940nm or 1050nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For axon imaging, power after the objective was limited to a maximum of 60 mW, dependent on depth. Locations were selected for imaging on the basis of their position relative to large blood vessels, responsiveness to visual stimulation, and lack of prolonged calcium transients resulting from over-expression of GCaMP6s. Images were collected at 30 Hz using bidirectional scanning with 512×512 pixel resolution. Images were collected at 512×512 pixel resolution with fields of view 100 (GCaMP6s) or 200 (jRGECO1a) μm on a side.
Scholl B., Wilson D.E., Jaepel J, & Fitzpatrick D. (2019). Functional logic of layer 2/3 inhibitory connectivity in ferret visual cortex. Neuron, 104(3), 451-457.e3.
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2

Silencing VIP Neurons with Optogenetics

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To silence VIP neurons, we used a 594-nm laser (OBIS 594 LS 100 mW, Coherent). We modified the Bergamo II microscope (Thorlabs) to combine optogenetic manipulation with two-photon calcium imaging. A lens (LA1805-B, Thorlabs) was placed in the optogenetic stimulation light path to defocus the light at the imaging plane. We used a dichroic mirror (DMBP740B, Thorlabs) to combine two-photon laser and optogenetic stimulation light, and a second dichroic mirror (FF555-Di03–25×36, Semrock) to split the green fluorescent protein (GFP) emission from both the two-photon and optogenetic light sources. The laser for optogenetic stimulation was synchronized to the resonant scanner turnaround points (when data were not acquired) to minimize light leak from the monitor (Attinger et al., 2017 (link)) and therefore flickered at twice the frequency of the resonant scanner. Trials with optogenetic stimulation, the laser was turned on 1 s before the visual stimulus and turned off 1 s after the offset of the visual stimulus. For all opsins, the average 594-nm laser power under the objective was typically set to a constant value of 15 mW (not exceeding 18 mW) throughout the trial.
Keller A.J., Dipoppa M., Roth M.M., Caudill M.S., Ingrosso A., Miller K.D, & Scanziani M. (2020). A Disinhibitory Circuit for Contextual Modulation in Primary Visual Cortex. Neuron, 108(6), 1181-1193.e8.
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3

Two-Photon Imaging of Biosensors

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Two-photon excitation of both biosensors was provided by the beam of an optical parametric oscillator (MPX Chameleon Compact OPO, Coherent, Inc., Santa Clara, CA, USA) tuned to 1025 nm, coupled to the Bergamo II microscope (Thorlabs, Inc.) and fed by a femtosecond pulsed Titanium-sapphire pump laser (Chameleon Ultra II Laser, Coherent, Inc.). The microscope was equipped with a water immersion objective designed for multiphoton imaging (XLPLN25XWMP2, 25×, NA 1.05, Olympus Corporation, Tokyo, Japan). Fluorescence emission signals were selected by band-pass filters (612/69 nm, Cat. No. FF01-612/69-25, Semrock, for R-CEPIA1er; 525/40 nm, Cat. No. FF02-525/40-25, Semrock, for CEPIA2mt) and detected by cooled GaAsP photomultiplier modules (Cat. No. H7422-40, Hamamatsu Photonics K.K., Shizuoka, Japan). Images were acquired simultaneously in these two emission channels at 3 frames/s (Hz).
Nardin C., Peres C., Mazzarda F., Ziraldo G., Salvatore A.M, & Mammano F. (2019). Photosensitizer Activation Drives Apoptosis by Interorganellar Ca2+ Transfer and Superoxide Production in Bystander Cancer Cells. Cells, 8(10), 1175.
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4

Two-photon Microscopy of Neural Structures

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Two-photon imaging was performed using a Bergamo II microscope (Thorlabs) running Scanimage (Vidrio Technologies) with 940 nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <50 mW. Images were collected at 30 Hz using bidirectional scanning with 512 × 512 pixel resolution or with custom ROIs (region of interest; framerate range: 22 – 50 Hz). Somatic imaging was performed with a resolution of 0.488 – 0.098 µm/pixel. Dendritic spine imaging was performed with a resolution of 0.164 – 0.065 µm/pixel.
Thomas C.I., Ryan M.A., McNabb M.C., Kamasawa N, & Scholl B. (2023). Astrocyte coverage of excitatory synapses correlates to measures of synapse structure and function in primary visual cortex. bioRxiv.
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5

Two-photon Imaging of Neurons

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Two-photon imaging was performed using a Bergamo II microscope (Thorlabs) running Scanimage (Vidrio Technologies) with 940 nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <50 mW. Images were collected at 30 Hz using bidirectional scanning with 512 × 512 pixel resolution or with custom ROIs (region of interest; framerate range: 22 – 50 Hz). Somatic imaging was performed with a resolution of 0.488 – 0.098 μm/pixel. Dendritic spine imaging was performed with a resolution of 0.164 – 0.065 μm/pixel.
Thomas C.I., Ryan M.A., Kamasawa N, & Scholl B. (2023). Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines. bioRxiv.
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6

Two-Photon Imaging of Neuronal Structures

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Two photon imaging was performed on a Bergamo II microscope (Thorlabs) running Scanimage36 2015 or 2016 (Vidrio Technologies) with 940nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <60 mW, dependent on depth. Cells were selected for imaging on the basis of their position relative to large blood vessels, responsiveness to visual stimulation, and lack of prolonged calcium transients resulting from overexpression of GCaMP6s. Images were collected at 30 Hz using bidirectional scanning with 512×512 pixel resolution. Images of somata ranged from 50 to 100 μm on a side, while images of dendrites were ~ 40μm on a side. Images of axons were collected at 512×512 pixel resolution with fields of view ~100μm on a side.
Wilson D.E., Scholl B, & Fitzpatrick D. (2018). Differential tuning of excitation and inhibition shapes direction selectivity in ferret V1. Nature, 560(7716), 97-101.
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7

Two-Photon Imaging in Neurophysiology

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Two-photon imaging was performed on a Bergamo II microscope (Thorlabs) running Scanimage33 (version 5, Vidrio Technologies) with 940 nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <50 mW. Cells were selected for imaging on the basis of their position relative to large blood vessels, responsiveness to visual stimulation, and lack of prolonged calcium transients resulting from over-expression of GCaMP6s. Images were collected at 30 Hz using bidirectional scanning with 512×512 pixel resolution or with custom ROIs (region of interest; framerate range: 22 – 50 Hz). Somatic imaging was performed with a resolution of 2.05 – 10.24 pixels/ μm. Dendritic spine imaging was performed with a resolution of 6.10 −15.36 pixels/μm.
Scholl B., Thomas C.I., Ryan M.A., Kamasawa N, & Fitzpatrick D. (2020). Cortical response selectivity derives from strength in numbers of synapses. Nature, 590(7844), 111-114.
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8

Two-Photon Imaging of Neuronal Structures

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-photon imaging was performed on a Bergamo II microscope (Thorlabs) running Scanimage (Pologruto et al., 2003 (link); Vidrio Technologies) with 940 nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <50 mW. Cells were selected for imaging on the basis of their position relative to large blood vessels, responsiveness to visual stimulation, and lack of prolonged calcium transients resulting from over-expression of GCaMP6s. Images were collected at 30 Hz using bidirectional scanning with 512 × 512 pixel resolution or with custom ROIs (frame rate range: 22–50 Hz). Somatic imaging was performed with a resolution of 2.05–10.24 pixels/micron. Dendritic spine imaging was performed with a resolution of 6.10–15.36 pixels/micron.
Yates J.L, & Scholl B. (2022). Unraveling Functional Diversity of Cortical Synaptic Architecture Through the Lens of Population Coding. Frontiers in Synaptic Neuroscience, 14, 888214.
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9

Two-Photon Imaging of Neuronal Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two photon imaging was performed on a Bergamo II microscope (Thorlabs) running Scanimage36 2015 or 2016 (Vidrio Technologies) with 940nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <60 mW, dependent on depth. Cells were selected for imaging on the basis of their position relative to large blood vessels, responsiveness to visual stimulation, and lack of prolonged calcium transients resulting from overexpression of GCaMP6s. Images were collected at 30 Hz using bidirectional scanning with 512×512 pixel resolution. Images of somata ranged from 50 to 100 μm on a side, while images of dendrites were ~ 40μm on a side. Images of axons were collected at 512×512 pixel resolution with fields of view ~100μm on a side.
Wilson D.E., Scholl B, & Fitzpatrick D. (2018). Differential tuning of excitation and inhibition shapes direction selectivity in ferret V1. Nature, 560(7716), 97-101.
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10

Two-Photon Imaging of Neural Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-photon imaging was performed using a Bergamo II microscope (Thorlabs) running Scanimage (ver. 5, Vidrio Technologies) with 940-nm dispersion-compensated excitation provided by an Insight DS+ (Spectraphysics). For spine and axon imaging, power after the objective was limited to <50 mW. Images were collected at 30 Hz using bidirectional scanning with 512 × 512 pixel resolution or with custom ROIs (region of interests; frame rate range: 22-50 Hz). Somatic imaging was performed with a resolution of 0.488-0.098 μm/pixel. Dendritic spine imaging was performed with a resolution of 0.164-0.065 μm/pixel.
Thomas C.I., Ryan M.A., Scholl B., Guerrero-Given D., Fitzpatrick D, & Kamasawa N. (2021). Targeting Functionally Characterized Synaptic Architecture Using Inherent Fiducials and 3D Correlative Microscopy. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27(1).
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