Bc2165

Approximately 100–150 mg fresh heart tissues were weighed, rinsed with cold normal saline to clean the blood, blotted with filter paper, and then cut into pieces. The extraction of mitochondria in the heart was performed using assay kits, according to the instructions of the manufacturer (SM0020, Solarbio, Beijing, China). The concentrations of mitochondrial isocitrate dehydrogenase (ICDHm, BC2165, Solarbio), succinate dehydrogenase (SDH, BC0955, Solarbio), NADP-malate dehydrogenase (NADP-MDH, BC1055, Solarbio), NADH oxidase (NOX, BC0635, Solarbio), and NADPH-cytochrome C reductase (NCR, BC2725, Solarbio) were measured according to the commercial detection kits. The activities of superoxide dismutase (SOD, BC0175, Solarbio), catalase (CAT, BC0205, Solarbio), glutathione (GSH, BC1175, Solarbio), glutathione s-transferase (GST, BC0355, Solarbio), and glutathione peroxidase (GPx, BC1195, Solarbio) were performed using assay kits, according to the instructions of the manufacturer.

The enhanced BCA protein assay kit, ATP and NADH content determination kit were purchased from Beyotime Institute of Biotechnology (catalog: P0012, S0026 and S0175, Shanghai, China). Fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), lipase (LPS), mitochondrial isocitrate dehydrogenase (ICDHm), NAD-malate dehydrogenase (NAD-MDH), succinate dehydrogenase (SDH), pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (α-KGDH), glutamine synthetase (GS), glutamate synthase (GOGAT), acetyl Co-A, and GSH assay kits were purchased from Beijing Solarbio Science & Technology Company Limited (Beijing, China), which catalog were BC0555, BC0415, BC2345, BC2165, BC1045, BC0955, BC0385, BC0715, BC0350, BC0075, BC0985, and BC1175, respectively. Chemical reagents were obtained by Shanghai Aladdin Bio-Chem Technology Company Limited (Shanghai, China). Deionized water (18.9 MΩ) was obtained by filtration of distilled water using a Milli-Q system (Millipore, MA, USA).