Kidneys were surgically isolated from C57BL/6 mice, washed 3 times in cold PBS, then dissected into 2–3 mm3 pieces using autoclaved sterilized scissors and tweezers. Minced kidney explants were suspended in Dulbecco’s modified Eagle Medium (DMEM, Gibco) with 10% fetal bovine serum (FBS, Corning) and cultured in a humidified atmosphere of 5% CO2 at 37°C(Luo et al., 2015 (link)). After 24 hours, culture media was changed to DMEM without 10% FBS and treated with DMOG (Selleckchem, #S7483), Chrysin (Selleckchem, #S2281), Fenofibrate (Selleckchem, #S1794), L-carnitine (Selleckchem, #S2388) in normoxia (21% O2,) or hypoxia condition (1% O2,). Hypoxic challenge was performed using hypoxia chamber (Whitley H45 Hypoxystation). After treatment, total RNA and protein were isolated for quantitative RT-PCR and western blot analysis.
Chrysin
Manufactured by Selleck Chemicals
Sourced in United States
Chrysin is a laboratory equipment product used for research and analysis. It functions as a chemical compound with specific properties and characteristics relevant to scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Fenofibrate, by Selleck Chemicals (1 mentions)
Dulbecco modified eagle medium (dmem), by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Selleck Chemicals (1 mentions)
L carnitine, by Selleck Chemicals (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Fisetin, by Selleck Chemicals (1 mentions)
Apigenin, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using chrysin
1
Kidney Explant Culture Assay
2
Developing Enz-Resistant Prostate Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human normal prostate epithelial cell line RWPE-1 and PCa cells LNCaP, C4-2, and 22Rv1 were cultured in Roswell Park Memorial Institute (RPMI) 1640 (Sigma Darmstadt, Germany) with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). All cell lines were cultured in a humid environment containing 5% CO2 and 95% air at 37 °C. The EnzR LNCaP and C4-2 cell lines were treated with Enz at 10, 20, 30, and then 40 µM until 20 days. Then, 10 µM Enz was added to make the cells resistant to Enz. After cell culture, the cell lines were treated with apigenin, chrysin, and fisetin (SelleckChem, Houston, TX, USA). These small molecules were added to the culture medium of PCa and EnzR PCa cells according to the manufacturer's instructions.
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