Transferrin

Manufactured by Abcam

Sourced in United States, United Kingdom

Transferrin is a glycoprotein that is responsible for the transport of iron in the body. It binds to and carries iron ions, delivering them to cells that require iron for various biological processes.

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Lab products found in correlation

Ferritin, by Abcam (1 mentions) Lactoferrin, by Abcam (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Tnf α, by R&D Systems (1 mentions) Anti pparγ e 8 sc 7273, by Santa Cruz Biotechnology (1 mentions) Anti adiponectin pa1 054 rabbit polyclonal antibody, by Thermo Fisher Scientific (1 mentions) Z vad fmk, by Merck Group (1 mentions) Slc7a11, by Abcam (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Glutaminase, by Abcam (1 mentions) Vimentin, by Abcepta (1 mentions) Slc40a1, by Abcam (1 mentions) Mmp 9, by Cell Signaling Technology (1 mentions) Liproxstatin 1, by Selleck Chemicals (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) Timp 2, by Abcam (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) β actin, by Merck Group (1 mentions) Donkey anti sheep igg, by Merck Group (1 mentions)

Spelling variants of this product

Transferrin receptor (9 citations) Anti-Transferrin (4 citations) Ab108911 kit for transferrin (3 citations) Transferrin Receptor (TfR, (2 citations) Human Transferrin ELISA kit (2 citations) Rabbit anti-Transferrin Receptor (2 citations) Transferrin (TF) (2 citations) Anti-transferrin receptor (2 citations)

12 protocols using transferrin

Plasma Biomarkers Quantification

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Plasma IL-6 (Invitrogen, Vienna, Austria), transferrin (Abcam, Cambridge, UK), ferritin (Abcam, Cambridge, UK) and G-CSF (MyBioSource, San Diego, CA) were measured by sandwich enzyme linked immunosorbent assay. All samples were run in duplicate following the manufacturer’s protocol.

Apple C.G., Miller E.S., Kannan K.B., Thompson C., Darden D.B., Efron P.A, & Mohr A.M. (2021). Prolonged Chronic Stress and Persistent Iron Dysregulation Prevent Anemia Recovery Following Trauma. The Journal of surgical research, 267, 320-327.

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Cytokine and Iron Biomarker Profiling

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Supernatants were centrifugated 10 min, 13,000×g, 4 °C. ELISAs were performed according to manufacturer’s instruction: human Hepcidin (R&D Systems, cat#DY8307-05), IL-6 (R&D Systems, cat#DY602), TNFα (R&D Systems, cat#DY2010), IL-8 (R&D Systems, cat#DY208), lactoferrin (abcam, cat#ab108882), and transferrin (abcam, cat#ab108902).

Hazlett H.F., Hampton T.H., Aridgides D.S., Armstrong D.A., Dessaint J.A., Mellinger D.L., Nymon A.B, & Ashare A. (2020). Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival. Scientific Reports, 10, 10935.

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Biomarker Validation in Lymphoma Subtypes

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We validated the proteins detected and their associated biological pathways by literature review, and selected three CSF proteins (haemopexin, apolipoprotein A1 and transferrin) as candidate biomarkers. Eighteen tissue samples used for analyses included six cases with nodal diffuse large B cell lymphoma (NDLBCL), six cases with primary CNS DLBCL, and six cases with normal lymph nodes.
Immunohistochemical staining involved a standard technique with the avidin-biotinylated peroxidase complex method. Apolipoprotein A1 antibody was diluted at 1:100 (Abcam, USA). Haemopexin and transferrin antibody were diluted at 1:200 (Abcam, USA). For each biomarker, images were scored visually by two pathologists, with more than 10% stained cells defined as positive. The degree of reactivity with each section was scored as the product of the scores for the intensity and extent of staining. The intensity of immunoreactivity was graded 0-3+ for no staining, weak, medium, and strong staining, respectively.

Zheng W., Song Y., Xie Y., Lin N., Tu M., Liu W., Ping L., Ying Z., Zhang C., Deng L., Wang X., Lu Y, & Zhu J. (2017). Cerebrospinal Fluid Proteins Identification Facilitates the Differential Diagnosis of Central Nervous System Diffuse Large B Cell Lymphoma. Journal of Cancer, 8(17), 3631-3640.

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Antibody Panel for OXPHOS and Adipogenesis

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Transferrin (ab82411; rabbit polyclonal) and the MitoProfile Total OXPHOS Rodent WB antibody mixture (ab110413; rabbit polyclonal) antibodies were purchased from Abcam (Cambridge, MA). Anti-PPARγ (E-8; sc-7273; mouse monoclonal) antibody was purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-adiponectin (PA1-054; rabbit polyclonal) antibody was purchased from Thermo Scientific (Waltham, MA). Anti-DBC1 (5693S; rabbit polyclonal), anti-FASN (3180S; C20G5; rabbit monoclonal), anti-ERK1/2 (4695S; 137F5; rabbit monoclonal), and anti-MYST1 (4682S; D5T3R; rabbit monoclonal) antibodies were purchased from Cell Signaling Technology (Danvers, MA).

Burrell J.A, & Stephens J.M. (2021). KAT8, lysine acetyltransferase 8, is required for adipocyte differentiation in vitro.. Biochimica et biophysica acta. Molecular basis of disease, 1867(6), 166103.

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Ferroptosis Regulation Protocol

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β-elemene (>98%) (#E4418) was purchased from LKT. Stock solution at 100 mg/ml was made in ethanol and stored at -4°C. Cetuximab (#33657) was purchased from MCE. Antibodies against GPX4 (#GR251529-34), HO-1 (#GR3187585-3), Glutaminase (#GR3299063-1), SLC40A1 (#GR215168-39), Transferrin (#GR3207592-9), SLC7A11 (#GR3235736-6) were purchased from Abcam. N-Cadherin (#13116S), E-Cadherin (#14472S), MMP9 (#13667S), snail (#3879T), slug (#9585T), and GAPDH (#2118S) antibodies were obtained from Cell Signaling Technology. Vimentin (#SA10106DB) was purchased from ABGENT. Ki67 (#66434-1-Ig) was purchased from Proteintech. Deferoxamine (#CS-4479), Ferrostain-1 (#HY-100579), Necrostain-1 (#HY-15760) were purchased from MCE. Z-VAD-FMK (#V116) was obtained from Sigma Aldrich. Liproxstatin-1 (#S7699) and RSL3 (#S8155) were purchased from Selleck Chemical.

Chen P., Li X., Zhang R., Liu S., Xiang Y., Zhang M., Chen X., Pan T., Yan L., Feng J., Duan T., Wang D., Chen B., Jin T., Wang W., Chen L., Huang X., Zhang W., Sun Y., Li G., Kong L., Chen X., Li Y., Yang Z., Zhang Q., Zhuo L., Sui X, & Xie T. (2020). Combinative treatment of β-elemene and cetuximab is sensitive to KRAS mutant colorectal cancer cells by inducing ferroptosis and inhibiting epithelial-mesenchymal transformation. Theranostics, 10(11), 5107-5119.

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Urinary Biomarkers for Acute Kidney Injury

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ELISA kits were used to measure urinary levels of KIM-1 (Cusabio, Wuhan, China), NGAL (Bioporto, Hellerup, Denmark), albumin (Abcam), transferrin (Abcam, Cambridge, UK), TIMP-2 (Abcam) and IGFBP-7 (Cloud Clone, Houston TX, USA), following the manufacturer instructions. The product of [TIMP-2]*[IGFBP7] urinary concentrations is marketed as NephroCheck®, and improves the diagnostic accuracy and outcomes of AKI patients. We have chosen these biomarkers due to previous studies by our research group^{21 (link)} and others^{36 (link),40 (link)} which show their increased urinary levels in AKI patients and in different experimental models of AKI.

Fuentes-Calvo I., Cuesta C., Sancho-Martínez S.M., Hidalgo-Thomas O.A., Paniagua-Sancho M., López-Hernández F.J, & Martínez-Salgado C. (2021). Biomarkers of persistent renal vulnerability after acute kidney injury recovery. Scientific Reports, 11, 21183.

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Quantifying Thyroid Hormone Levels

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Total T3 (tT3) and T4 (tT4) levels as well as transferrin concentrations in serum were analyzed applying an enzyme-linked immunosorbent assay kit (tT3 and tT4: Abnova Corporation, Taipei City, Taiwan; transferrin: Abcam, Cambridge, UK). To this end, serum samples were either applied undiluted (tT3), after a 1:2 (tT4) or 1:200,000 dilution (transferrin) to the microplate, following manufacturer’s instructions. The average absorbance was measured at 450 nm using a microplate reader (Synergy H1, BioTek, Bad Friedrichshall, Germany). Calculation of concentrations was performed based on standard curves.
TSH levels in serum were measured by using the Mouse Pituitary Magnetic Bead Panel-Endocrine Multiplex Assay (MPTMAG-49K, Millipore, Darmstadt, Germany) and a Luminex 200 device, based on the Luminex xMAP technology. Assay was performed in accordance with the manual. Average CV [%] of all included samples was <10%.

Lossow K., Renko K., Schwarz M., Schomburg L., Schwerdtle T, & Kipp A.P. (2021). The Nutritional Supply of Iodine and Selenium Affects Thyroid Hormone Axis Related Endpoints in Mice. Nutrients, 13(11), 3773.

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Cerebral Cortex Protein Analysis

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Proteins were extracted from the cerebral cortices (bregma +2 to −3 mm) and the plasma 24 h after stroke. The two assays were conducted as described.^{23 (link),27 (link)} The following primary antibodies were used for Western blotting: MMP-3 (1:1000; Abcam); β-actin (1:1000; Sigma); transferrin (1:1000; Abcam). Semiquantitative assessment of immunoblots and MMP bands was performed by computerized densitometry.

Liu S., Jin R., Wang M, & Li G. (2020). Nanoparticle Delivery of CD147 Antagonistic Peptide-9 Protects against Acute Ischemic Brain Injury and tPA-Induced Intracerebral Hemorrhage in Mice. ACS applied bio materials, 3(4), 1976-1985.

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Caveolin-1 Dynamics in TNBC Cells

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Cells from both TNBC cell lines were pre-incubated in serum-free media and treated with 0.5 mM MβCD for 48 hours. Treated cells were subjected to 1% Triton X-100 followed by OptiPrep gradient separation to isolate detergent resistant membrane (DRM) and non-DRM fractions. A specific enzyme linked immunosorbent assay (ELISA) kit was used to analyze caveolin-1 (MyBioSource, San Diego, USA) and transferrin levels (Abcam, Cambridge, USA).

Badana A., Chintala M., Varikuti G., Pudi N., Kumari S., Kappala V.R, & Malla R.R. (2016). Lipid Raft Integrity Is Required for Survival of Triple Negative Breast Cancer Cells. Journal of Breast Cancer, 19(4), 372-384.

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Plasma Biomarkers in Alzheimer's Disease

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Plasma from AD patients and non-demented (ND) controls was obtained from the University of Kentucky Sanders-Brown Center on Aging (Group 1) and Washington University Knight Alzheimer’s Disease Research Center (Group 2). Group 1 AD cases were defined by clinical diagnosis of AD as well as postmortem Consortium to Establish a Registry for Alzheimer’s Disease (CERAD) neuritic plaque score [44 (link)] of B or C, corresponding to probable or definite AD, respectively. ND cases had CERAD score 0 and no clinical diagnosis of AD (Supplemental Table 1). Group 2 AD cases had a Clinical Dementia Rating score (CDR; measuring cognitive function) [45 (link)] of ≥0.5 and CSF Aβ42 levels <500 pg/ml, and ND cases had a CDR score of 0 and CSF Aβ42 levels >500 pg/ml (Supplemental Table 2). For Group 1, blood was drawn into heparinized plastic Vacutainer tubes. For Group 2, blood was drawn using EDTA-coated syringes into polypropylene tubes containing a final concentration of 5 mM EDTA.
Equal amounts of total protein from each sample (as determined by BCA) were analyzed by Western blot with antibodies against FXI (HTI), C1 esterase inhibitor (Proteintech), fibrin beta chain (59D8 [46 (link)]), D-dimer (AbD Serotec) and transferrin (Abcam). Purified FXI, C1 esterase inhibitor (Athens Research and Technology), and FXI-deficient plasma (George King Biomedical) served as controls.

Zamolodchikov D., Renné T, & Strickland S. (2016). The Alzheimer’s disease peptide Aβ promotes thrombin generation through activation of coagulation factor XII. Journal of thrombosis and haemostasis : JTH, 14(5), 995-1007.

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