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Anti neuropilin1

Manufactured by Abcam
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Sourced in United Kingdom

Anti-Neuropilin1 is a lab equipment product. It functions as an antibody that specifically binds to the Neuropilin-1 protein.

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Lab products found in correlation

Polyvinylidene fluoride microporous membrane, by Bio-Rad (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti rac1, by Abcam (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Protease inhibitor cocktail, by Beyotime (1 mentions) Bradford protein assay, by Bio-Rad (1 mentions) Anti β actin, by Fortis Life Sciences (1 mentions) Gradient gel, by Bio-Rad (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti lamp2, by Santa Cruz Biotechnology (1 mentions) Anti ki67 antibody, by Agilent Technologies (1 mentions) Anti vegfr2 antibody, by Thermo Fisher Scientific (1 mentions) Anti cd31, by Abcam (1 mentions)

3 protocols using anti neuropilin1

1

Protein Expression Analysis by Western Blot

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Cells were lysed in RIPA buffer (Beyotime, Jiangsu, China) containing protease inhibitor cocktail (Beyotime). Total protein was quantified by the Bradford protein assay (Bio-Rad, Hercules, CA). Samples were denatured at 100 °C for 5 min. Equal amounts of total protein were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting using polyvinylidene fluoride microporous membranes (Bio-Rad). Membranes were incubated with primary antibody overnight at 4 °C, followed by incubation with horseradish peroxidase-linked secondary antibody at room temperature for 1 hour and detected with SuperSignal West Pico Chemiluminescent Substrate (Thermo). The primary antibodies, anti-Rac1 and anti-Neuropilin1 were purchased from Abcam (Cambridge, UK), while anti-β-catenin and anti-GAPDH were purchased from Cell Signaling Technology (Danvers, MA).
Zhou J., Zhang M., Huang Y., Feng L., Chen H., Hu Y., Chen H., Zhang K., Zheng L, & Zheng S. (2014). MicroRNA-320b promotes colorectal cancer proliferation and invasion by competing with its homologous microRNA-320a. Cancer letters, 356(2 0 0), 669-675.
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2

Neuropilin-1 Protein Expression Analysis

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Protein was extracted from mouse cortex and mixed with sample buffer (5% 2-mercaptoethanol) and boiled at 100℃ for denaturation. Protein samples were loaded on 4-15% gradient gel (Bio-Rad) to separate on size through vertical SDS-PAGE system. Antibodies used for immune-blot analysis were; anti-Neuropilin-1 (abcam, 1:1000) and anti-β-actin (BETHYL, 1:10000). Images were acquired by ChemiDoc MP imaging system (Bio-Rad).
Lim K., Yang S., Kim S., & Joo J. (2021). Identifying new COVID-19 receptor Neuropilin-1 in severe Alzheimer’s diseases patients group brain using genome-wide association study approach.
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3

Antibody Validation for VEGFR2 Signaling

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Anti-SCUBE2 (catalog GX85173) polyclonal antibody was from GeneTex (Irvine, CA). Anti-phospho-VEGFR2 (Thr1059; catalog 3817), anti-phospho-MAPK p44/p42 (Thr202/Tyr204; catalog 9106), anti-MAPK p44/p42 (catalog 9102), anti-phospho-AKT (Ser473; catalog 9271), and anti-AKT (catalog 9272) antibodies were from Cell Signaling Technology (Danvers, MA). Anti-VEGFR2 antibody was from Thermo Scientific (Rockford, IL; catalog MA5-15157). Anti-VEGF (catalog sc-152), anti-EEA1 (early endosome antigen 1; catalog sc-137130), and anti-LAMP2 (lysosome-associated membrane protein 2; catalog sc-18822) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CD31 (catalog ab28364) and anti-neuropilin-1 (catalog ab81321) antibodies were from Abcam (Cambridge, MA). Anti-Ki67 antibody was from DAKOCytomation (Glostrup, Denmark; catalog M7249). Recombinant VEGF 165 protein was from R&D Systems (Minneapolis, MN; catalog 293-VE).
Lin Y.C., Liu C.Y., Kannagi R, & Yang R.B. (2018). Inhibition of Endothelial SCUBE2 (Signal Peptide-CUB-EGF Domain-Containing Protein 2), a Novel VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) Coreceptor, Suppresses Tumor Angiogenesis. Arteriosclerosis, thrombosis, and vascular biology, 38(5).
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