Water h2o

Manufactured by Thermo Fisher Scientific

Sourced in Belgium, United States

Water (H2O) is a colorless, odorless, and tasteless chemical compound composed of two hydrogen atoms and one oxygen atom. It is a vital substance that serves as the universal solvent in various scientific and laboratory applications.

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Lab products found in correlation

Ethyl acetate, by Avantor (2 mentions) Glacial acetic acid, by Avantor (2 mentions) Phosphate buffered saline (pbs), by Merck Group (2 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Succinic acid 2 2 3 3 d4, by Merck Group (1 mentions) 1 13c pyruvic acid, by Merck Group (1 mentions) Dl alanine 15n, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Avantor (1 mentions) U 13c6 d glucose, by Cambridge Isotopes (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (1 mentions) 1 2 dipentadecanoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 tridecanoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) Acetonitrile mecn, by Thermo Fisher Scientific (1 mentions) Methanol meoh, by Thermo Fisher Scientific (1 mentions) Formic acid, by Biosolve (1 mentions) α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions) Maldi matrix, by Merck Group (1 mentions) Hematoxylin and eosin h e staining kit, by Thermo Fisher Scientific (1 mentions) Citraconic anhydride, by Thermo Fisher Scientific (1 mentions)

8 protocols using water h2o

Optimized Metabolite Sample Prep

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Solvents and reagents used in sample preparation and mobile phase were of Optima LC-MS-grade. Methanol (MeOH), water (H₂O), and formic acid were purchased from Fisher Scientific (Illkirch, France). Isotope labeled endogenous metabolite standards (at a minimal purity of 98%), namely 17α-Hydroxyprogesterone-d8 (2,2, 4,6,6,21,21,21-d8), L-Thyroxine-13C6, Succinic acid-2,2,3,3-d4, Pyruvic acid-1-13C, and DL-Alanine-15N were purchased from Sigma Aldrich (St. Quentin Fallavier, France). A working solution at a concentration of 10 μg/mL of each isotope labeled standards was prepared in MeOH.

Kouassi Nzoughet J., Guehlouz K., Leruez S., Gohier P., Bocca C., Muller J., Blanchet O., Bonneau D., Simard G., Milea D., Procaccio V., Lenaers G., Chao de la Barca J.M, & Reynier P. (2020). A Data Mining Metabolomics Exploration of Glaucoma. Metabolites, 10(2), 49.

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UPLC Analysis of Antibiotic Compound

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Analytical grade Ultra Performance Liquid Chromatography (UPLC) solvents were used: acetonitrile (ACN), methanol (MeOH) and water (H₂O) from Fisher Scientific (Erembodegem, Belgium), ethyl acetate and glacial acetic acid from VWR (Leuven, Belgium). The analytical standard of FF and internal standard thiamphenicol (TIA) were purchased from Sigma-Aldrich (Diegem, Belgium). Standard stock solutions of 1.0 mg/mL were prepared in a H₂O/MeOH solution (50/50 v/v) and were stored airtight and protected from light at 4–8°C for a maximal period of 3 weeks. Phosphate-buffered saline (PBS) was obtained from Sigma-Aldrich (Diegem), and sodium hydroxide (NaOH) from VWR (Leuven).

De Smet J., Boyen F., Croubels S., Rasschaert G., Haesebrouck F., De Backer P, & Devreese M. (2018). Similar Gastro-Intestinal Exposure to Florfenicol After Oral or Intramuscular Administration in Pigs, Leading to Resistance Selection in Commensal Escherichia coli. Frontiers in Pharmacology, 9, 1265.

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Lipidomic Analysis of Metabolites

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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE(16:0/18:1(9Z))), 1,2-dipentadecanoyl-sn-glycero-3-phosphoethanolamine (PE(15:0/15:0)), 1-tridecanoyl-sn-glycero-3-phosphoethanolamine (LysoPE(13:0)), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (PS(16:0/18:1(9Z))),
1,2-diheptadecanoyl-sn-glycero-3-phospho-l-serine (PS(17:0/17:0)), and 1-tridecanoyl-sn-glycero-3-phospho-l-serine (LysoPS(13:0)) were purchased from Avanti Polar Lipids
(Alabaster, AL). [U-¹³C₆]-d-glucose
(99%) was purchased from Cambridge Isotope Laboratories (Tewksbury,
MA). Butylated hydroxytoluene 2,6-di-tert-butyl-p-cresol (BHT) and all solvents, including MeOH, acetonitrile
(ACN), isopropanol (IPA), chloroform (CHCl₃), and water
(H₂O), of LC-MS grade and high performance liquid chromatography
(HPLC) grade were purchased from Fisher Scientific (Pittsburgh, PA).

He X, & Zhang Q. (2018). Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids. ACS Omega, 3(11), 15725-15733.

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Phosphoric Acid Analytical Protocol

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Phosphoric acid (H₃PO₄) analytical grade was purchased from Sigma-Aldrich (Buchs, Switzerland). Formic acid (FA) was obtained from Biosolve (Valkenswaard, The Netherlands), and acetonitrile (MeCN), water (H₂O), and methanol (MeOH) from Fisher Scientific (Loughborough, UK). All the solvents and additives were UPLC-MS grade.

Olesti E., Boccard J., Rahban R., Girel S., Moskaleva N.E., Zufferey F., Rossier M.F., Nef S., Rudaz S, & González-Ruiz V. (2023). Low-polarity untargeted metabolomic profiling as a tool to gain insight into seminal fluid. Metabolomics, 19(6), 53.

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Mass Spectrometry Tissue Imaging

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Analytical grade solvents, acetonitrile (ACN) and water (H₂O) were purchased from Fisher Scientific (Pittsburgh, PA, USA). Histological grade xylene and ethanol, and the MALDI matrix, alpha-cyano-4-hydroxycinnamic acid (CHCA) were purchased from Sigma Aldrich (St. Louis, MO). Hematoxylin and eosin (H&E) staining kit, citraconic anhydride for antigen retrieval and positively charged glass slides were purchased from Thermo Scientific (San Jose, CA, USA). Recombinant Peptide N-Glycosidase F (PNGase F) was obtained from the laboratory of Dr. Richard Drake (Charleston, NC, USA) and the commercial version, PNGase F PRIME™, was purchased from N-zyme Scientifics (Doylestown, PA, USA).

Carter C.L., Parker G.A., Hankey K.G., Farese A.M., MacVittie T.J, & Kane M.A. (2020). MALDI-MSI spatially maps N-glycan alterations to histologically distinct pulmonary pathologies following irradiation. Scientific Reports, 10, 11559.

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UPLC-MS Metabolite Extraction Protocol

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UPLC-MS grade acetonitrile (ACN), methanol (MeOH), and water (H₂O) were obtained from Fisher Scientific ( Loughborough, UK). UPLC-MS grade formic acid (FA) was supplied by Biosolve (Valkenswaard, Netherlands). Chloroform (CHCl₃) was provided by Acros Organics (Geel, Belgium). Ammonium hydroxide and ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) were obtained from Sigma-Aldrich (Buchs, Switzerland). The major mix IMS/T of calibration kit and leucine-encephalin were acquired from Waters (Milford, MA, USA).

Pezzatti J., Bergé M., Boccard J., Codesido S., Gagnebin Y., H. Viollier P., González-Ruiz V, & Rudaz S. (2019). Choosing an Optimal Sample Preparation in Caulobacter crescentus for Untargeted Metabolomics Approaches. Metabolites, 9(10), 193.

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Optimized LC-MS/MS for Glyphosate and AMPA

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All solvents were high-performance liquid chromatography (HPLC) grade and suitable for trace analysis. Acetonitrile (ACN), dichloromethane (DCM), methanol (MeOH) and water (H₂O) were purchased from Fisher Scientific (Hampton, NH).
For optimization of the liquid chromatography with tandem mass spectrometry (LC-MS/MS) method, glyphosate-FMOC (Dr Ehrenstorfer, 95.8 % purity) and AMPA-FMOC (Dr Ehrenstorfer, 99.89 % purity) were purchased from LGC Standards, (Manchester, NH, USA). Glyphosate (1000 μg/ml in H₂O, 99 % purity) and AMPA (100 μg/ml in H₂O, 99 % purity) solutions were purchased from RESTEK (State College, PA, USA). Glyphosate-1,2-¹³C₂,¹⁵N (100 μg/g in H₂O, Dr Ehrenstorfer, 97 % combined purity) and AMPA-¹³C,¹⁵N (100 μg/mL in H₂O, Dr Ehrenstorfer, 97 % combined purity) solutions were purchased from LGC Standards (Manchester, NH, USA).
Ammonium acetate (for molecular biology, ≥ 98 %), formic acid (puriss. p.a., ACS reagent, reag. Ph. Eur., ≥ 98 %) and hydrochloric acid (ACS reagent, 37 %) were purchased from Sigma Aldrich.

Cruz J.M, & Murray J.A. (2020). Determination of glyphosate and AMPA in oat products for the selection of candidate reference materials. Food chemistry, 342, 128213.

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HPLC-MS/MS Quantification of Enrofloxacin

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All solvents used were of analytical grade; acetonitrile (ACN), methanol (MeOH), water (H₂O) from Fisher Scientific (Erembodegem, Belgium), glacial acetic acid and ethyl acetate from VWR (Leuven, Belgium). Standards of ENR and internal standard (IS) ENR-d5 were purchased from Sigma-Aldrich (Diegem, Belgium) and prepared in a H₂O/MeOH solution (50/50 v/v). These stock solutions of 1 mg/mL were stored airtight and protected from light at ≤ − 15 °C for a maximal period of 60 days. Phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich (Diegem, Belgium).

De Smet J., Boyen F., Croubels S., Rasschaert G., Haesebrouck F., Temmerman R., Rutjens S., De Backer P, & Devreese M. (2020). The impact of therapeutic-dose induced intestinal enrofloxacin concentrations in healthy pigs on fecal Escherichia coli populations. BMC Veterinary Research, 16, 382.

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