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R23E10-Gal4 is a genetic tool used in Drosophila research. It is a Gal4 driver line that expresses the Gal4 transcription factor in a specific pattern in the Drosophila nervous system.

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Lab products found in correlation

Uas cschrimson mvenus, by Bloomington Drosophila Stock Center (3 mentions) Canton s, by Bloomington Drosophila Stock Center (1 mentions) Tub gal80ts, by Bloomington Drosophila Stock Center (1 mentions) Uas denmark, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) 104y gal4, by Bloomington Drosophila Stock Center (1 mentions)

7 protocols using r23e10 gal4

1

Rearing and Manipulating Drosophila Flies

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D. melanogaster flies were reared in vials (groups of 20 flies/vial) on standard yeast-sugar-agar based media (1.0–1.5–0.5 g ratio) under a 12:12 light/dark (8 AM:8 PM) cycle and maintained at 25°C with 50% humidity. Adult, 3–5-day-old female, flies were used for all experiments and randomly assigned to experimental groups. Fly lines used for behavioral and RNA-sequencing experiments include R23E10-Gal4 (attp2; Bloomington 49032; Bloomington Drosophila Stock Center, Bloomington, IN) and UAS-CsChrimson-mVenus (attp18; Bloomington 55134; Provided by Janelia Research Campus, Ashburn, VA) (Klapoetke et al., 2014 (link)). For all two-photon experiments, flies with the genotype 10XUAS-Chrimson88-tdTomato (attp18)/+:LexAop-nlsGCaMP6f (VIE-260b; kindly provided by Barry J. Dickson)/+:Nsyb-LexA (attP2) (Pfeiffer et al., 2012 (link)), LexAop-PAGFP (VK00005)/R23E10-Gal4 were used. Optogenetically manipulated fly lines were maintained on food containing 0.5 mg/ml ATR (Merck, Darmstadt, Germany) for 24 hr prior to assay to allow for sufficient consumption. Pharmacologically manipulated flies were maintained on food with 0.1 mg/ml of gaboxadol (THIP) for the duration of behavioral experiment (Dissel et al., 2015 (link)).
Anthoney N., Tainton-Heap L., Luong H., Notaras E., Kewin A.B., Zhao Q., Perry T., Batterham P., Shaw P.J, & van Swinderen B. (2023). Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila. eLife, 12, RP88198.
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2

Optogenetic Manipulation of Fruit Flies

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Drosophila melanogaster flies were reared in plastic vials on standard yeast-based medium under a 12:12 light/dark (8 AM:8 PM) cycle and maintained at 25°C with 50% humidity. Adult, 3–5 day-old female flies were used for all experiments and randomly assigned to experimental groups. Fly lines used for behavioral experiments were R23E10-Gal4 (attp2; Bloomington 49032; Bloomington Drosophila Stock Center, Bloomington, Indiana), UAS-CsChrimson-mVenus (attp18; Bloomington 55134; Provided by Janelia Research Campus, Ashburn, Virginia), and Canton-S (Bloomington 64349). For 2-photon experiments, flies with the genotype 10XUAS-Chrimson88-tdTomato (attp18) / +: LexAop-nlsGCaMP6f (VIE-260b); kindly provided by Barry J. Dickson) / +: Nsyb-LexA (attP2), LexAop-PAGFP (VK00005) / R23E10-Gal4 were used. Optogenetically-manipulated fly lines were maintained on food containing 0.2mM all-trans retinal (ATR; Merck, Darmstadt, Germany) for 24 hours prior to assay to allow for sufficient consumption.
Tainton-Heap L.A., Kirszenblat L.C., Notaras E.T., Grabowska M.J., Jeans R., Feng K., Shaw P.J, & van Swinderen B. (2020). A paradoxical kind of sleep in Drosophila melanogaster. Current biology : CB, 31(3), 578-590.e6.
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3

Drosophila Behavioral Manipulation and Neuroimaging

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Drosophila melanogaster flies were reared in vials (groups of 20 flies / vial) on standard yeast-sugar-agar based media (1.0–1.5–0.5 gram ratio) under a 12:12 light/dark (8 AM:8 PM) cycle and maintained at 25°C with 50% humidity. Adult, 3–5 day-old female, flies were used for all experiments and randomly assigned to experimental groups. Fly lines used for behavioral and RNA-sequencing experiments include R23E10-Gal4 (attp2; Bloomington 49032; Bloomington Drosophila Stock Center, Bloomington, Indiana) and UAS-CsChrimson-mVenus (attp18; Bloomington 55134; Provided by Janelia Research Campus, Ashburn, Virginia)[31 (link)]. For all 2-photon experiments, flies with the genotype 10XUAS-Chrimson88-tdTomato (attp18) / +: LexAop-nlsGCaMP6f (VIE-260b; kindly provided by Barry J. Dickson) / +: Nsyb-LexA (attP2) [32 (link)], LexAop-PAGFP (VK00005) / R23E10-Gal4 were used. Optogenetically-manipulated fly lines were maintained on food containing 0.5mg/ml all-trans retinal (ATR; Merck, Darmstadt, Germany) for 24 hours prior to assay to allow for sufficient consumption. Pharmacologically-manipulated flies were maintained on food with 0.1 mg/ml of Gaboxadol (4,5,6,7-tetrahyrdoisoxazolopyridin-3-ol, THIP) for the duration of behavioral experiment [23 (link)].
Anthoney N., Tainton-Heap L.A., Luong H., Notaras E., Kewin A.B., Zhao Q., Perry T., Batterham P., Shaw P.J, & van Swinderen B. (2023). Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila. bioRxiv.
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4

Rearing and Manipulating Drosophila Flies

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D. melanogaster flies were reared in vials (groups of 20 flies/vial) on standard yeast-sugar-agar based media (1.0–1.5–0.5 g ratio) under a 12:12 light/dark (8 AM:8 PM) cycle and maintained at 25°C with 50% humidity. Adult, 3–5-day-old female, flies were used for all experiments and randomly assigned to experimental groups. Fly lines used for behavioral and RNA-sequencing experiments include R23E10-Gal4 (attp2; Bloomington 49032; Bloomington Drosophila Stock Center, Bloomington, IN) and UAS-CsChrimson-mVenus (attp18; Bloomington 55134; Provided by Janelia Research Campus, Ashburn, VA) (Klapoetke et al., 2014 (link)). For all two-photon experiments, flies with the genotype 10XUAS-Chrimson88-tdTomato (attp18)/+:LexAop-nlsGCaMP6f (VIE-260b; kindly provided by Barry J. Dickson)/+:Nsyb-LexA (attP2) (Pfeiffer et al., 2012 (link)), LexAop-PAGFP (VK00005)/R23E10-Gal4 were used. Optogenetically manipulated fly lines were maintained on food containing 0.5 mg/ml ATR (Merck, Darmstadt, Germany) for 24 hr prior to assay to allow for sufficient consumption. Pharmacologically manipulated flies were maintained on food with 0.1 mg/ml of gaboxadol (THIP) for the duration of behavioral experiment (Dissel et al., 2015 (link)).
Anthoney N., Tainton-Heap L., Luong H., Notaras E., Kewin A.B., Zhao Q., Perry T., Batterham P., Shaw P.J, & van Swinderen B. (2023). Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila. eLife, 12, RP88198.
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5

Drosophila Behavior Imaging Protocols

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Fruit flies (Drosophila melanogaster) were raised at 25°C in 50–60% relative humidity on standard medium containing cornmeal, yeast, glucose, wheat germ, and agar, as described before [9 (link)]. They were maintained under a 12-h light: dark (LD) cycle. In this study, we used R52B10-Gal4 (BDSC stock number 38820), R23E10-Gal4 (49032), R23E10-LexA (52693), R52B10-LexA (52826), UAS-mCD8::GFP (5130), tub-Gal80ts (7019), UAS-GCaMP6s (42746), UAS-DenMark, syt.eGFP (33064), LexAop-P2X2 (76030), hDeltaC-Gal4 (75925), and vDeltaB, C, D-Gal4 (93172) from the Bloomington Drosophila Stock Center, and NP2320-Gal4 (DGRC stock number 104157) from the Drosophila Genetics Resource Center. UAS-dTrpA1 [12 (link)] was a gift from Dr. Julie H. Simpson. Cha-Gal80 [13 (link)] was from Dr. Takaomi Sakai. UAS-CD4::spGFP1-10, LexAop- CD4::spGFP11 were from Dr. Kristin Scott. UAS-Kir2.1 [14 (link)] was from Dr. Richard A. Baines. R52B10-Gal4, R23E10-Gal4, NP2320-Gal4, and UAS-dTrpA1 are backcrossed at least 5 times to the control strain (w1118). Male flies were used in all experiments.
Kato Y.S., Tomita J, & Kume K. (2022). Interneurons of fan-shaped body promote arousal in Drosophila. PLOS ONE, 17(11), e0277918.
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6

Optogenetic Manipulation of Neuronal Circuits in Flies

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Flies were cultured on standard agar medium under a 12-hr day/night cycle. Flies used for optogenetics were placed on food containing 1 mM all-trans retinal (Sigma) for two days before experiments. All flies were outcrossed six generations to a w2202 genetic background (isoCJ1; (Yin et al., 1994 (link)). The INX6-RNAi lines were a gift from Chia-Lin Wu. UAS-CsChrimson was a gift from Vivek Jayaraman. R23E10-Gal4, C5-Gal4, 104y-Gal4, and UAS-shibire were obtained from Bloomington. The INX6 RNAi is v8638, originally obtained from the VDRC (Dietzl et al., 2007 (link)). UAS-syx3-69 was obtained from Bing Zhang.
Troup M., Yap M.H., Rohrscheib C., Grabowska M.J., Ertekin D., Randeniya R., Kottler B., Larkin A., Munro K., Shaw P.J, & van Swinderen B. (2018). Acute control of the sleep switch in Drosophila reveals a role for gap junctions in regulating behavioral responsiveness. eLife, 7, e37105.
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7

Drosophila Rearing and Manipulation Protocols

Cited 1 time
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Drosophila melanogaster flies were reared in vials (groups of 20 flies / vial) on standard yeast-sugar-agar based media (1.0–1.5–0.5 gram ratio) under a 12:12 light/dark (8 AM:8 PM) cycle and maintained at 25°C with 50% humidity. Adult, 3–5 day-old female, flies were used for all experiments and randomly assigned to experimental groups. Fly lines used for behavioral and RNA-sequencing experiments include R23E10-Gal4 (attp2; Bloomington 49032; Bloomington Drosophila Stock Center, Bloomington, Indiana) and UAS-CsChrimson-mVenus (attp18; Bloomington 55134; Provided by Janelia Research Campus, Ashburn, Virginia)[31 (link)]. For all 2-photon experiments, flies with the genotype 10XUAS-Chrimson88-tdTomato (attp18) / +: LexAop-nlsGCaMP6f (VIE-260b; kindly provided by Barry J. Dickson) / +: Nsyb-LexA (attP2) [82 (link)], LexAop-PAGFP (VK00005) / R23E10-Gal4 were used. Optogenetically-manipulated fly lines were maintained on food containing 0.5mg/ml all-trans retinal (ATR; Merck, Darmstadt, Germany) for 24 hours prior to assay to allow for sufficient consumption. Pharmacologically-manipulated flies were maintained on food with 0.1 mg/ml of Gaboxadol (4,5,6,7-tetrahyrdoisoxazolopyridin-3-ol, THIP) for the duration of behavioral experiment [23 (link)].
Anthoney N., Tainton-Heap L.A., Luong H., Notaras E., Kewin A.B., Zhao Q., Perry T., Batterham P., Shaw P.J, & van Swinderen B. (2023). Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila. bioRxiv.
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