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Recombinant human tnfα af 300 01a

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Recombinant human TNFα (AF-300-01A) is a laboratory reagent used for research purposes. It is a recombinant version of the human tumor necrosis factor alpha (TNFα) protein. TNFα is a cytokine involved in the regulation of various cellular processes.

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Lab products found in correlation

Cycloheximide (chx), by Selleck Chemicals (1 mentions) Cisplatin hy 17394, by MedChemExpress (1 mentions) Tnfr1, by Proteintech (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using recombinant human tnfα af 300 01a

1

Investigating TNFα-induced NF-kB Signaling

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Recombinant human TNFα (AF-300-01A) was purchased from PeproTech. Cisplatin (HY-17394) was from MedChemExpress, QNZ (S4902) and Cycloheximide (CHX) was from Selleck. The primary antibodies used in this work are as follow: KIAA1522 (WB, 1:1000; IHC, 1:200; HPA032050, Sigma ImmunoChemicals, St Louis, MO, USA), Phospho-NF-kB p65 (Ser536) (WB, 1:1000; CST, 3033); NF-κB p65 (WB, 1:1000; CST, 8242), Phospho-IkBα (Ser32) (WB, 1:1000; CST, 2859), IkBα (WB, 1:1000; CST, 4814), Phospho-IKKα/β (Ser176/180) (WB, 1:1000; IHC, 1:150; CST, 2697), IKKα (WB, 1:1000, CST, 11930), TNFR1 (WB, 1:1000, Proteintech, 21,574–1-AP), TNFR2 (WB, 1:1000; IHC, 1:50, Proteintech, 19,272–1-AP), GAPDH (WB, 1:1000, Santa Cruz, sc-25,778), β-actin (WB, 1:2000, Santa Cruz, sc-47,778), Ki67 (IHC, 1:200, GeneTex, GTX16667), cleaved caspase-3 (IHC, 1: 500, CST, 9664).
Wang B., Jing T., Jin W., Chen J., Wu C., Wang M, & Liu Y. (2020). KIAA1522 potentiates TNFα-NFκB signaling to antagonize platinum-based chemotherapy in lung adenocarcinoma. Journal of Experimental & Clinical Cancer Research : CR, 39, 170.
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2

Recombinant Human TNF-α Sourcing

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Recombinant human TNF-α (AF-300-01A) was purchased from PeproTech (Cranbury, NJ, USA).
Gornalusse G.G., Vojtech L.N., Levy C.N., Hughes S.M., Kim Y., Valdez R., Pandey U., Ochsenbauer C., Astronomo R., McElrath J, & Hladik F. (2021). Buprenorphine Increases HIV-1 Infection In Vitro but Does Not Reactivate HIV-1 from Latency. Viruses, 13(8), 1472.
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