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12 protocols using cobalt 2 nitrate hexahydrate

1

Synthesis of Transition Metal Compounds

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Potassium permanganate (VII) (KMnO4 powder, ≥99.0 %), manganese (II) nitrate tetrahydrate (Mn(NO3)2·4H2O, ≥97%) and cobalt (II) nitrate hexahydrate (Co(NO3)2·6H2O, ≥97%) purchased from Sinopharm, were used as received without further purification.
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2

Dopamine-loaded Zn-based Metal-Organic Nanoparticles

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Zinc acetate dihydrate (Zn(OAC)2·2H2O, 99.99%) and dopamine hydrochloride (AR, 98.0%,) were purchased from Aladdin Corp. Cetyltrimethylammonium bromide (CTAB, 99%) and triblock poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) Pluronic F-127 (Mav = 12,600) were purchased from Sigma-Aldrich. Tris(hydroxymethyl)aminomethane (Tris, ≥99.5%), 2-methylimidazole (1-MeIM, AR, ≥98.0%), zinc nitrate hexahydrate (Zn(NO3)2·6H2O, AR, ≥99.0%), cobalt (II) nitrate hexahydrate (Co(NO3)2·2H2O, ≥ 98.5%,), 1,3,5-trimethylbenzene (TMB, ≥ 99.5%), hydrochloric acid (HCl, 36.0–38.0%), 2,3,6-trimethylphenol (TMP, 98.0%), methanol anhydrous (AR, ≥99.5%), hydrogen peroxide (30 wt.%), ammonium hydroxide solution (28 wt.% NH3 in H2O), polyvinylpyrrolidone K30 (GR), copper(II) chloride dihydrate(AR, ≥99.0%) and anhydrous ethanol (AR, ≥99.7%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Disulfiram (97%) was purchased from Aladdin Reagent Co. Ltd. Fetal bovine serum (FBS), penicillin-streptomycin, trypsin, and RPMI 1640 medium were provided by Gibco Life Technologies Co. Cell Counting Kit-8 (CCK-8), calcein-AM/propidium iodide (PI) and annexin V-FITC Apoptosis Detection Kit were obtained from Beyotime Biotech (China).
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3

Synthesis of Metal Oxide Nanomaterials

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Toluene (98%, Sinopharm
Chemical Reagent Co., Ltd.), nitric acid (68%, Sinopharm Chemical
Reagent Co., Ltd.), ammonia (ADAMAS-BETA), sulfuric acid (98%, Sinopharm
Chemical Reagent Co., Ltd.), zirconium(III) oxide octahydrate (ZrOCl2·8H2O), cerium(III) nitrate hexahydrate [Ce(NO3)3·6H2O, 98%, Sinopharm Chemical
Reagent Co., Ltd.], cobalt(II) nitrate hexahydrate [Co(NO3)2·6H2O, 98%, Sinopharm Chemical Reagent
Co., Ltd.], manganese(II) nitrate tetrahydrate [Mn(NO3)2·4H2O, 98%, ADAMAS-BETA], and zinc nitrate
hexahydrate [Zn(NO3)2·6H2O,
98%, Sinopharm Chemical Reagent Co., Ltd.] were used in this study.
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4

Synthesis of Manganese-Cobalt Oxide Electrocatalyst

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All chemicals employed in this experiment were acquired from commercial dealers of analytical rank and were utilized as received without additional purification. Manganese sulfate monohydrate (99 %, MnSO4.H2O, India (Sisco Research Laboratories Pvt. Ltd.)), Cobalt (II) nitrate hexahydrate (99.5%, Co(NO3)2.6H2O) and ammonia (China (Sinopharm Chemical Reagent Co., Ltd.)). Sodium hydroxide (NaOH, 99.5%)), Vulcan XC 72 carbon powder (96.5%), potassium hydroxide (97.5%, KOH), potassium permanganate (99.5%, KMnO4), ethanol (98.5%), and sodium carbonate (97.5%) were obtained from Alpha Chemika (India). Throughout the experiments distilled water (DW) was employed as the solvent.
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5

Synthesis of Cobalt-Nickel-Selenium Catalysts

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Cobalt (II) nitrate hexahydrate (Co(NO3)2·6H2O, AR), nickel (II) nitrate hexahydrate (Ni(NO3)2·6H2O, AR), 2-methylimidazole (2-MI, C4H6N2, AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. N,N-Dimethylformamide (C3H7NO, DMF, AR) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Selenium powder (Se, 99%) and polyacrylonitrile (PAN, Mw¯ = 150,000, 99%) were purchased from Shanghai Macklin Biochemical Co., Ltd. All chemicals were of analytical grade and used without further purification.
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6

Graphene Oxide Synthesis and Functionalization

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Flake graphite powder (C), phosphorus pentoxide (P2O5, AR), potassium peroxydisulfate (K2S2O8, AR), concentrated sulfuric acid (H2SO4, AR), potassium permanganate (KMnO4, AR), hydrogen peroxide (H2O2, AR), Cobalt(ii) nitrate hexahydrate (Co(NO3)2·6H2O, AR), 2-methylimidazole (2-MIM, C4H6N2, 98%), ascorbic acid(Vc, C6H8O6, AR), sodium hypophosphate monohydrate (NaH2PO2·H2O, AR) bought from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
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7

Synthesis of Nanostructured Catalysts

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Hydrochloric acid (HCl), Urea (99 wt.%), Hydrazine monohydrate (N2H4·H2O, 80 wt.%), Cobalt(II) nitrate hexahydrate (Co(NO3)2•6H2O, 98.5 wt.%) and Nickel(II) nitrate hexahydrate (Ni(NO3)2•6H2O, 98.0 wt.%) were purchased from Sinopharm Group Chemical Reagent Co., Ltd. Sodium hypophosphite (NaH2PO2, 99.0 wt.%) and Ammonium fluoride (NH4F, 98 wt.%) were purchased from Aladdin. Sodium hydroxide (KOH, 90 wt.%) was purchased from Shanghai Titan Scientific Co., Ltd. Acetone, Ethanol, Isopropanol were purchased from Shanghai Lingfeng. Nafion D-520 dispersion (5 wt.%) was purchased from Dupont China Holding Co., Ltd. Commercial 20 wt.% Pt/C and the carbon black (XC-72) were purchased from Shanghai HEPHAS Energy Equipment Co., Ltd. Nickel foam (thickness: 1.0 mm; aperture: 0.1 mm; porosity: 97.2%) was purchased from Cyber Electric Co., Ltd. Anion exchange membrane (AEM, FAB-PK-130) was purchased from Fumasep. Composite matrix was purchased from Changsha Sipulin. All materials were used as received without further purification.
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8

Synthesis of Metal Nitrate Nanocomposites

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Nickel(ii) nitrate hexahydrate [Ni(NO3)2·6H2O, 98.0%], cobalt(ii) nitrate hexahydrate [Co(NO3)2·6H2O, 99.0%], iron(ii) nitrate nonahydrate [Fe(NO3)2·9H2O, 98.0%], ammonium fluoride [NH4F, ≥98.0%] and urea [CO(NH2)2, ≥98.0%] were procured from Sinopharm Chemical Reagent Co., Ltd, while oleylamine [C18H37N, 99.0%] and ethanol (C2H5OH; 99.9 wt%) were purchased from Aladdin Reagent Co. Ltd. Potassium hydroxide [KOH, ≥95%] was obtained from Alfa Aesar. The deionized (DI) water used in this study was obtained from a Millipore Autopure system with a resistivity of 18.25 MΩ cm−1. All other materials were of analytical grade and did not require further purification before use.
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9

Synthesis of Ni/Co-Based Nanocomposites

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Nickel(II)
nitrate hexahydrate (Ni(NO3)2·6H2O), cobalt(II) nitrate hexahydrate (Co(NO3)2·6H2O), urea (CH4N2O), ammonium
thiotungstate (H8N2S4W), N,N-dimethylformamide (DMF), PVA, and KOH
were purchased from Sinopharm Chemical Reagent Co., Ltd. All the chemical
reagents were AR grade and used without further purification. CC (WOS1002
from CeTech) was obtained and treated with double distilled water
and ethanol.
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10

Cobalt-Zinc Heterojunction for Cancer Therapy

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Zinc nitrate hexahydrate (Zn(NO3)2·6H2O), cobalt(II) nitrate hexahydrate (Co(NO3)2·6H2O), and 2-methylimidazole were procured from Sinopharm Chemical Reagent Co., Ltd. The cobra cytotoxin (CTX) was sourced from the Snake Venom Research Institute of Guangxi Medical University. Chlorin e6 (Ce6), SPDP, and SH-RGD were acquired from Xi’an Ruixi Biotechnology Co., Ltd. The Calcein/PI, JC-1 reagent test kit, Annexin V-FITC/PI apoptosis assay kit, and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were kindly supplied by Novozymes. Liver cancer cell lines (HepG2) and human normal liver cells (THLE-2) were obtained from the Chinese Academy of Medical Sciences, Beijing.
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