Iscript advanced reverse transcriptase kit

Alamar Blue® powder was obtained from Sigma-Aldrich Co. (St. Louis, MO). Dulbecco’s modified Eagle’s medium (DMEM) high glucose, fetal bovine serum heat-inactivated (FBS-HI), and penicillin/streptomycin were obtained from Genesee Scientific (San Diego, CA, USA). Mouse cytokine antibody arrays (cat# AAM-CYT-1000) and ELISA assays were purchased from RayBiotech (Norcross, Ga, USA). PCR primers, iScript advanced reverse transcriptase kit, and SYBR Green were purchased from Bio-Rad (Hercules, CA, USA). Turbo DNA-free™ Kit (Cat# AM1907) from Life Technologies Inc. (Grand Island, NY, USA).

MDA-MB-231 (derived from Caucasian American TNBC) and MDA-MB-468 (derived from African American TNBC) were purchased from American Type Culture Collection (ATCC). Dulbecco’s modified Eagle’s medium (DMEM) high glucose; fetal bovine serum heat inactivated (FBS-HI), penicillin/streptomycin and Hank’s Balanced salt solution (HBSS) were obtained from Genesee Scientific (San Diego, CA, USA). Dimethyl sulfoxide (DMSO), butein, and Alamar Blue^® were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Human cytokine antibody arrays (Cat# AAH-CYT-6-4), ELISA assays for MCP-1 (Cat# ELH-MCP1–1), Annexin V-FITC apoptosis Kit (Cat# 68FT-AnnV-S100), and tumor necrosis factor alpha (TNF-α) were purchased from RayBiotech (Norcross, Ga, USA). PCR primers, iScript advanced reverse transcriptase kit, and Bradford reagent were purchased from Bio-Rad (Hercules, CA, USA). DNA-free^™ Kit (Cat # AM1907) from Life Technologies Inc. (Grand Island, NY, USA).). All reagents and plates for Western assays were purchased from ProteinSimple (San Jose, CA, USA). Primary antibodies were purchased from Cell Signaling (Danvers, MA, USA). The list of primary antibodies and their characteristics are described as follows (Table 1):

MDA-MB-231 (Caucasian American) and MDA-MB-468 (African American) TNBC cells were purchased from the American Type Culture Collection (ATCC). Heat-inactivated fetal bovine serum (HI-FBS), Dulbecco’s modified Eagle’s medium (DMEM) high glucose, penicillin/streptomycin, and phosphate-buffered saline (PBS) were obtained from Genesee Scientific (San Diego, CA, USA). Alamar Blue^®, fucoxanthin (95% purity), dimethyl sulfoxide (DMSO), chloroform, and isopropyl alcohol were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Human Angiogenesis Antibody Array (Cat# AAH-ANG-1000-4) and TNF-alpha (Cat# ELH-TNFα) were purchased from RayBiotech (Norcross, GA, USA). TRIzol was purchased from Thermo Fischer Scientific (Wilmington, DE, USA). PrimePCR_PI3K-AKT signaling pathway (SABTarget list) H96 arrays, SYBR Green, and iScript advanced reverse transcriptase kit were purchased from Bio-Rad (Hercules, CA, USA). A propidium iodide DNA staining kit was purchased from Abcam USA (Cambridge, MA, USA). The Wes kit and reagents used were obtained from ProteinSimple (San Jose, CA, USA). The antibodies used were obtained from Cell Signaling Technology (Danvers, MA, USA) and ThermoFisher (Waltham, MA, USA).