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τ spad 100

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The τ-SPAD 100 is a single-photon avalanche diode (SPAD) detector designed for time-resolved photon-counting applications. It offers a high photon detection efficiency and low noise, making it suitable for various scientific and industrial applications that require the detection of single photons with high temporal resolution.

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Lab products found in correlation

Uplsapo 100xo, by Olympus (2 mentions) Ldh d c 640, by PicoQuant (2 mentions) Labview 2009, by National Instruments (2 mentions) P 517.3cl, by Physik Instrumente (2 mentions) Razoredge lp 647, by IDEX Corporation (2 mentions) Uplsapo 60xo, by Olympus (2 mentions) Spc 830, by Becker & Hickl (2 mentions) Ldh d c 485, by PicoQuant (1 mentions) Symphotime program, by PicoQuant (1 mentions) Microtime 200, by PicoQuant (1 mentions)

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τ-SPAD (11 citations)

3 protocols using τ spad 100

1

Single-molecule Fluorescence Imaging Setup

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Single-molecule fluorescence transients are measured with a custom built confocal setup based on an inverted microscope (IX71, Olympus) with a high NA oil immersion objective (60X, NA 1.35, UPLSAPO 60XO for rectangular DNA origami; 100x/NA 1.40, UPLSAPO100XO for DNA nanopillar and ZMWs, both Olympus). The ATTO647N dye molecules are excited at 640 nm with an 80 MHz pulsed laser diode (LDH-D-C-640, Picoquant, 2.5 μW for rectangular DNA origami, 1.5 μW for DNA nanopillar measurements, 5 μW for ZMW measurements). A combination of linear polarizer and quarter wave plate ensures circular polarization of the laser beam which is guided to the sample by a dichroic beamsplitter (Dualband z532/633, AHF).The surface with immobilized DNA origamis is imaged by scanning the sample with a piezo stage (P-517.3CL, Physik Instrumente). From that image, molecules are selected and placed in the laser focus for time resolved analysis. The resulting fluorescence is collected by the same objective and separated from the excitation light after focusing on a 50 μm pinhole by two filters (Bandpass ET 700/75m, AHF; RazorEdge LP 647, Semrock). The signal is detected by a single photon counting module (τ-SPAD 100, Picoquant) and a PC-card for time-correlated single-photon counting (SPC-830, Becker&Hickl). The raw data is processed with custom made software (LabView2009, National Instruments).
Holzmeister P., Pibiri E., Schmied J.J., Sen T., Acuna G.P, & Tinnefeld P. (2014). Quantum yield and excitation rate of single molecules close to metallic nanostructures. Nature communications, 5, 5356.
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2

Confocal FLIM Microscopy of Microstructures

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Images were recorded using a confocal fluorescence microscope (Microtime 200, PicoQuant) system equipped with a frequency doubled picosecond pulse diode laser (485 nm, 100 ps, 40 MHz, LDH-D-C-485, PicoQuant). The FLIM system was used with configuration explained in ref. 36 . Microstructures were fabricated near the surface of PMMA at 250 nJ, scan speed of 1 mm/s using 0.25 NA objective. The sample was placed on top of a clean coverslip positioned onto an oil immersion TIR objective (100X, NA 1.45, Olympus, PLAPO) and a beam splitter (500dcxr, Chroma) was used to reflect the excitation light onto the objective after collimation by a fiber optic cable. The epifluorescence signal was passed through a 510 nm long pass filter (Chroma). The fluorescence intensity images and lifetime traces were recorded using a single-photon-counting module (τ-SPAD-100, PicoQuant) and Time Correlated Single Photon Counting Module (TSCPC), and processed through SymPhoTime program (PicoQuant).
Kallepalli D.L., Alshehri A.M., Marquez D.T., Andrzejewski L., Scaiano J.C, & Bhardwaj R. (2016). Ultra-high density optical data storage in common transparent plastics. Scientific Reports, 6, 26163.
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3

Single-molecule Fluorescence Imaging Setup

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-molecule fluorescence transients are measured with a custom built confocal setup based on an inverted microscope (IX71, Olympus) with a high NA oil immersion objective (60X, NA 1.35, UPLSAPO 60XO for rectangular DNA origami; 100x/NA 1.40, UPLSAPO100XO for DNA nanopillar and ZMWs, both Olympus). The ATTO647N dye molecules are excited at 640 nm with an 80 MHz pulsed laser diode (LDH-D-C-640, Picoquant, 2.5 μW for rectangular DNA origami, 1.5 μW for DNA nanopillar measurements, 5 μW for ZMW measurements). A combination of linear polarizer and quarter wave plate ensures circular polarization of the laser beam which is guided to the sample by a dichroic beamsplitter (Dualband z532/633, AHF).The surface with immobilized DNA origamis is imaged by scanning the sample with a piezo stage (P-517.3CL, Physik Instrumente). From that image, molecules are selected and placed in the laser focus for time resolved analysis. The resulting fluorescence is collected by the same objective and separated from the excitation light after focusing on a 50 μm pinhole by two filters (Bandpass ET 700/75m, AHF; RazorEdge LP 647, Semrock). The signal is detected by a single photon counting module (τ-SPAD 100, Picoquant) and a PC-card for time-correlated single-photon counting (SPC-830, Becker&Hickl). The raw data is processed with custom made software (LabView2009, National Instruments).
Holzmeister P., Pibiri E., Schmied J.J., Sen T., Acuna G.P, & Tinnefeld P. (2014). Quantum yield and excitation rate of single molecules close to metallic nanostructures. Nature communications, 5, 5356.
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