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Pi annexin 5 fluorescein isothiocyanate

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PI)-Annexin V/fluorescein isothiocyanate is a fluorescent conjugate used to detect and quantify apoptosis in cell populations. It binds to phosphatidylserine, which is exposed on the cell surface during programmed cell death.

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Lab products found in correlation

Annexin 5 pi, by BD (1 mentions) Facscalibur, by BD (1 mentions) Cytomics fc500, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Annexin V-Fluorescein isothiocyanate (FITC)/Propidium lodide (PI) Apoptosis Kit Annexin-V fluorescein isothiocyanate/PI dual stain Annexin V-fluorescein isothiocyanate/PI apoptosis detection kit Annexin V–fluorescein isothiocyanate (FITC)/PI staining kit Annexin V-fluorescein isothiocyanate (FITC)/PI apoptosis detection kit Annexin V-fluorescein isothiocyanate (FITC)/PI apoptosis detection Annexin V-fluorescein isothiocyanate/PI apoptosis detection Annexin V-fluorescein isothiocyanate (FITC)/PI kit Annexin V-fluorescein isothiocyanate (FITC)/PI Annexin V-fluorescein isothiocyanate propidium iodide (Annexin V-FITC/PI) kit

5 protocols using pi annexin 5 fluorescein isothiocyanate

1

Cell Cycle and Apoptosis Analysis

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Cells investigated (5 × 105 cells/well) were grown to 80% confluence, rinsed with cold PBS, and collected by scraping for flow cytometry.
Cell cycle: After pre-chilled PBS washes, cells were fixed with pre-chilled 70% ethanol for 2 h at 4℃. After 30 min of staining with propidium iodide (PI) solution, cells were then detected using a flow cytometer.
Apoptosis: Collected cells were stained with Annexin V-Fluorescein Isothiocyanate/PI according to the manuals (BD Pharmingen, USA). Detection was performed using a flow cytometer after 15 min. The assay was repeated for three times.
Long J., Zhu B., Tian T., Ren L., Tao Y., Zhu H., Li D, & Xu Y. (2023). Activation of UBEC2 by transcription factor MYBL2 affects DNA damage and promotes gastric cancer progression and cisplatin resistance. Open Medicine, 18(1), 20230757.
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2

Evaluating Apoptosis in Neonate Rat Myocardiocytes

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The apoptosis was evaluated by FTIC‐Annexin V/PI kit as previously reported.25 Briefly, the isolated neonate rat myocardiocytes were suspended (1 × 106 cells/mL) and incubated with Annexin V‐fluorescein isothiocyanate/PI (BD Biosciences) for 15 minutes. After incubation, the apoptotic myocardiocytes were evaluated by a flow cytometer (FACSCalibur, BD Biosciences) and analysed by CellQuest software version 3.3.
Dai S.H., Wu Q.C., Zhu R.R., Wan X.M, & Zhou X.L. (2020). Notch1 protects against myocardial ischaemia‐reperfusion injury via regulating mitochondrial fusion and function. Journal of Cellular and Molecular Medicine, 24(5), 3183-3191.
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3

Apoptosis and Mitochondrial Potential Assessment

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Cells were stained with propidium iodide (PI)-Annexin V/fluorescein isothiocyanate or phycoerythrin-Annexin V/7-amino-actinomycin (BD BioSciences, San Jose, CA, USA) according to the manufacturer’s instructions. JC-1 (10 μg ml−1; BD BioSciences) was used to measure mitochondrial membrane potential. Cells were then analysed using a flow cytometer (Cytomics FC500; Beckman Coulter, Fullerton, CA, USA).
Li S., Li J., Dai W., Zhang Q., Feng J., Wu L., Liu T., Yu Q., Xu S., Wang W., Lu X., Chen K., Xia Y., Lu J., Zhou Y., Fan X., Mo W., Xu L, & Guo C. (2017). Genistein suppresses aerobic glycolysis and induces hepatocellular carcinoma cell death. British Journal of Cancer, 117(10), 1518-1528.
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4

Apoptosis Assessment by Flow Cytometry

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For assessment of apoptosis, cells were incubated with propidium iodide (PI)/annexin V–fluorescein isothiocyanate (BD Biosciences) for 20 min at room temperature and then analyzed by flow cytometry.
Hiraki M., Suzuki Y., Alam M., Hinohara K., Hasegawa M., Jin C., Kharbanda S, & Kufe D. (2016). MUC1-C Stabilizes MCL-1 in the Oxidative Stress Response of Triple-Negative Breast Cancer Cells to BCL-2 Inhibitors. Scientific Reports, 6, 26643.
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5

Cell Apoptosis Assessment by Flow Cytometry

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H9 and HuT-78 cells were incubated with propidium iodide (PI)/annexin V-fluorescein isothiocyanate (BD BioSciences) for 15 minutes at room temperature and then analyzed by flow cytometry. Each experiment was done in triplicate and repeated thrice.
Jain S., Washington A., Leaf R.K., Bhargava P., Clark R.A., Kupper T.S., Stroopinsky D., Pyzer A., Cole L., Nahas M., Apel A., Rosenblatt J., Arnason J., Kufe D, & Avigan D. (2017). Decitabine Priming Enhances Mucin 1 Inhibition Mediated Disruption of Redox Homeostasis in Cutaneous T-cell Lymphoma. Molecular cancer therapeutics, 16(10), 2304-2314.
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