Human cortical astrocytes were obtained from Sciencell (Carlsbad, CA, USA) and cultured in astrocyte media supplemented with astrocyte growth serum, FBS, and pen/strep (Sciencell, Carlsbad, CA, USA). Astrocytes were passaged and split onto cell culture plates coated with 0.1% gelatin (Bio-Rad, Hercules, CA, USA). All neurons were cultured on coverslips on top of astrocyte-coated plates excluding the 24-hour morphology experiments.
Human cortical astrocytes
Manufactured by ScienCell
Sourced in United States
Human cortical astrocytes are primary glial cells derived from the cerebral cortex of the human brain. They serve core functions in the central nervous system, including providing structural and metabolic support for neurons, regulating neurotransmitter levels, and modulating synaptic transmission.
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2 protocols using human cortical astrocytes
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Culturing Human Cortical Astrocytes
2
Fluorescent Labeling of Cell Lines
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The human breast cancer cell line MDA-MB-231 and human dermal fibroblasts were obtained from ATCC (Manassas, VA, USA) and human cortical astrocytes were obtained from Sciencell (Carlsbad, CA, USA). MDA-MB-231 cells and fibroblasts were cultured and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS. Astrocytes were cultured in supplemented astrocyte medium (Sciencell, Carlsbad, CA, USA). Cells were labeled with fluorescent CellTracker Dyes (Thermo Scientific, Waltham, MA, USA) at recommended dilutions in serum-free media for one hour before use.
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