Ab97959

For the BrdU immuno-labeling, the brain sections were rinsed three times in 0.1 M PBS and incubated in 2 N HCl at 37°C for 15 min, followed by 0.1 M borate buffer (pH = 8.6) for 10 min and washed 3× with 0.1 M PBS. Sections were incubated in blocking solution for 40 min. Then, sections were incubated with some combinations of the following primary antibodies: rat anti-BrdU (1:500; AbD Serotec Cat # OBT0030, RRID:AB_609568), mouse anti-NeuN (1:500; Millipore Cat # MAB377, RRID:AB_2298772), rabbit anti-GFAP (1:100; Dako Cat # Z0334, RRID:AB_10013382), rabbit anti-Sox2 (1:500, Abcam Cat # AB97959; RRID:AB_2341193), guinea pig anti-doublecortin (DCX, 1:1000: Millipore Cat# AB2253, RRID:AB_1586992) in blocking solution at 4°C overnight. Sections were rinsed 3× with 0.1 M PBS, and incubated in 0.1 M PBS containing 10% fetal bovine serum and conjugated secondary antibodies (Alexa Fluor^® 488 anti-rat Cat # A-21208; Alexa Fluor^® 594 anti-rat Cat # A-11007; Alexa Fluor^® 488 anti-mouse Cat # A32723; Alexa Fluor^® 594 anti-rabbit; Alexa Fluor^® 594 Cat# R37117; anti-guinea pig Cat# A-11076; dilution 1:1000; Thermo Fisher) for 1 h at room temperature and washed 3× with 0.1 M PBS. Nuclear counterstaining was done with 4′,6-diamidino-2-phenylindole (DAPI; Abcam Cat # ab104139, Cambridge, MA, USA).