All chemicals and reagents were of analytical grade. Folin–Ciocalteu phenol reagent, sodium carbonate, gallic acid, porcine pancreatic lipase, 4-methylumbelliferone, phosphate buffer saline, sodium citrate, orlistat, taurocholic acid, p-nitrophenylbutyrate (p-NPB), sodium phosphate buffer, sodium chloride, porcine pancreatic cholesterol esterase, simvastatin, cholesterol, oleic acid, phosphatidylcholine, methanol, and taurocholate salt were purchased from Sigma-Aldrich (St. Louis, MO).
Porcine pancreatic cholesterol esterase
Manufactured by Merck Group
Sourced in United States
Porcine pancreatic cholesterol esterase is an enzyme derived from porcine (pig) pancreas. It catalyzes the hydrolysis of cholesterol esters, breaking down the ester bond between cholesterol and a fatty acid.
Automatically generated - may contain errors
Lab products found in correlation
Porcine pancreatic lipase, by Merck Group (3 mentions)
Orlistat, by Merck Group (3 mentions)
Oleic acid, by Merck Group (3 mentions)
Taurocholic acid, by Merck Group (2 mentions)
Phosphatidylcholine, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Cholesterol, by Merck Group (2 mentions)
Sodium phosphate buffer, by Merck Group (1 mentions)
Simvastatin, by Merck Group (1 mentions)
4 methylumbelliferone, by Merck Group (1 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
P nitrophenyl butyrate p npb, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Sodium citrate, by Merck Group (1 mentions)
Sodium glycodeoxycholate, by Merck Group (1 mentions)
Epigallocatechin gallate (egcg), by Merck Group (1 mentions)
Cholestyramine resin, by Merck Group (1 mentions)
4 protocols using porcine pancreatic cholesterol esterase
1
Lipase and Cholesterol Esterase Assays
2
Evaluation of Anti-Lipase and Anti-Cholesterol Agents
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Porcine pancreatic lipase (PPL, type II), 4-nitrophenyl butyrate (p-NPB), porcine pancreatic cholesterol esterase, oleic acid, phosphatidylcholine, cholesterol, sodium taurocholate hydrate, sodium chenodeoxycholate, sodium glycodeoxycholate, orlistat, epigallocatechin gallate (EGCG), cholestyramine resin, cinnamyl acetate, eugenol, kaempferol, trans-cinnamaldehyde, trans-cinnamic acid, phlorizidin, epicatechin, catechin, 4-hydroxybenzoic acid, gallic acid, and HMG-CoA reductase assay kits (CS 1090) were purchased from Sigma-Aldrich Co., St. Louis, MO, USA. Total cholesterol test kits (BXC0261) were purchased from Fortress Diagnostics, UK, and total bile acid kits (BQ 042A-EALD) were purchased from Bio-Quant Co. (San Diego, CA, USA). All the other chemical reagents used in this study were of analytical grade.
3
Lipase Activity Assay Protocol
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Porcine pancreatic lipase, phosphate buffer (PBS, pH 7.4), p-nitrophenyl butyrate, dimethylformamide, taurocholic acid, porcine pancreatic cholesterol esterase, orlistat, penicillin, streptomycin, bovine serum albumin (BSA), oleic acid, Oil Red-O, isopropanol, formalin, aspirin, sodium phosphate monobasic, and sodium phosphate dibasic dihydrate were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA).
4
Cholesterol Esterase Activity Assay
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All chemicals employed in this study, including the culture medium, trichloroacetic acid, soy lecithin, β-sitosterol, trypsin, porcine pancreatic cholesterol esterase, phosphate salts, and boron trifluoride, were sourced from Sigma Aldrich (Shanghai) Trading Co., Ltd., Shanghai, China. Tween-80, sodium hydroxide, sodium chloride, n-heptane, anhydrous sodium sulfate, Triton X-100, methanol, phosphotungstic acid, p-nitrophenyl butyrate, taurocholate buffer and imidazole were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. The reagents utilized were of ACS grade. The preparation of all solutions was executed utilizing purified and deionized ultra-pure water.
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