Flavonol standards

HPLC grade solvents (ethanol, formic acid, and acetonitrile) were obtained from Merck (Santiago, Chile). Ultrapure water was obtained from a water system of purification (Milli-Q Merck Millipore, Chile). Flavonol standards (catechin, isoflavones, and flavonoids, with a high purity: 95% by HPLC) were acquired from ChromaDex (Santa Ana, CA, USA), Sigma-Aldrich (Saint Louis, Mo, USA), or Extrasynthèse (Genay, France). Folin-Ciocalteu reagent, NaOH, Na₂CO₃, AlCl₃, FeCl₃, HCl, NaNO₂, trichloroacetic acid, quercetin, Trolox sodium acetate, gallic acid, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), nitroblue tetrazolium, xanthine oxidase, and DPPH (1,1-diphenyl-2-picrylhydrazyl radical), acetylcholinesterase (AChE) from electric eel (Torpedo californica), butylcholinesterase (BChE) from horse serum, 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB), acetylthiocholine iodide, butyrylthiocholine chloride, and galantamine were purchased from Sigma-Aldrich Chemical Company (Santiago, Chile). G. sphacelata fruits were collected in November 2016 in Valdivia, XIV Region de Los Ríos, Chile. The fruits were identified by Jorge Macaya (Universidad de Chile) and a voucher specimen were kept at the Institute of Pharmacy of the Universidad Austral de Chile under number GS20161115.

Solvents type UHPLC-MS, formic acid type LC-MS, and chloroform were from Merck (Santiago, Chile). Ultrapure water was obtained from a water purification system brand Millipore (Milli-Q Merck Millipore, Chile). Flavonol standards, (catechin, isoflavones, and flavonoids, all standards with a high purity: 95% by HPLC) were acquired from ChromaDex (Santa Ana, CA, United States), Sigma Aldrich (Saint Louis, Mo, United States), or Extrasynthèse (Genay, France). Folin-Ciocalteu reagent, NaOH, Na₂CO₃, AlCl₃, FeCl₃, HCl, NaNO₂, trichloroacetic acid, quercetin, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), sodium acetate, Gallic acid, TPTZ, nitroblue tetrazolium, DPPH (1,1-diphenyl-2-picrylhydrazyl radical), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), 2,2’-Azobis (2-amidinopropane) dihydrochloride (AAPH), were acquired from Sigma-Aldrich Chemical Company (Santiago, Chile).