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Alexa 594 conjugated anti mouse igg antibody

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The Alexa Fluor 594-conjugated anti-mouse IgG antibody is a secondary antibody used for the detection and visualization of mouse primary antibodies in various immunoassays. The antibody is conjugated to the Alexa Fluor 594 dye, which provides bright and photostable fluorescence.

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Lab products found in correlation

Donkey serum, by Jackson ImmunoResearch (3 mentions) 4 6 diamidino 2 phenylindole dapi d9542, by Merck Group (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Fluoromount g, by Southern Biotech (1 mentions) Eclipse fluorescence microscope, by Nikon (1 mentions) M4276, by Merck Group (1 mentions)

Close spelling variants of this product

Alexa 594 (538 citations) Alexa Fluor 594-conjugated goat anti-mouse IgG antibody (11 citations) Alexa-Fluor 594-conjugated anti-mouse IgG antibody (10 citations) Alexa Fluor 594-conjugated goat anti-mouse IgG secondary antibody (10 citations) Mouse anti-IgG (2 citations) Anti-mouse IgG Alexa Fluor® 594-conjugated secondary antibody (2 citations)

4 protocols using alexa 594 conjugated anti mouse igg antibody

1

Myogenic Differentiation of MDSCs

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To test the myogenic differentiation capacity, the MDSCs were seeded on
24 well plates (3×104cells/well) in proliferation medium and
then the next day switched to myogenic differentiation medium (DMEM supplemented
with 2% FBS and 1% PS). Three days after plating, immunocytochemical staining
for fast myosin heavy chain (MyHCf) was performed. The cells were fixed in cold
methanol (−20°C) for 5 minutes, blocked with 10% donkey serum
(Jackson ImmunoResearch, 017-000-121) for 1 hour, and then incubated with a
mouse anti-MyHCf (Sigma-Aldrich, M4276, 1:250) antibody for 2 hours at RT. The
primary antibody was detected with an Alexa 594-conjugated anti-mouse IgG
antibody (Invitrogen, A21203, 1:500;) for 30 minutes. The nuclei were revealed
by 4, 6-diamidino-2-phenylindole (DAPI, D9542, 100ng/ml, Sigma-Aldrich)
staining. The percentage of differentiated myotubes was quantified as the number
of nuclei in MyHCf positive myotubes relative to the total number of nuclei.
Pferdehirt L., Guo P., Lu A., Huard M., Guilak F, & Huard J. (2022). In Vitro Analysis of Genome-Engineered Muscle Derived Stem Cells for Autoregulated Anti-Inflammatory and Anti-Fibrotic Activity. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 40(12), 2937-2946.
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2

Immunocytochemical Quantification of Myotube Differentiation

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The muscle cells were plated on 24 well plates (30,000 cells/well) in DMEM supplemented with 2% FBS to promote myogenic differentiation (myotube formation). Three days after plating, immunocytochemical staining for fast myosin heavy chain (MyHCf) was performed. After rinsing two times with PBS, cells were fixed for 5 minutes in cold methanol (−20 °C), blocked with 10% donkey serum (Jackson ImmunoResearch, 017–000–121) for 1 hour, and then incubated with a mouse anti-MyHCf (Sigma-Aldrich, M4276, 1:250) antibody for 2 hours at RT. The primary antibody was detected with an Alexa 594-conjugated anti-mouse IgG antibody (Invitrogen, A21203, 1:500) for 30 minutes. The nuclei were revealed by 4, 6-diamidino-2- phenylindole (DAPI, D9542, 100 ng/ml, Sigma-Aldrich) staining. The percentage of differentiated myotubes was quantified as the number of nuclei in MyHCf positive myotubes relative to the total number of nuclei.
Lu A., Guo P., Wang L., Tseng C., Huard M., Allen C., McCarrick-Walmsley R., Whitney K.E, & Huard J. (2020). Heterogenetic parabiosis between healthy and dystrophic mice improve the histopathology in muscular dystrophy. Scientific Reports, 10, 7075.
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3

Visualizing HIV-1 p24 in Transfected HeLa Cells

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HeLa cells seeded on coverslips were transfected with a plasmid containing 24xMS2 binding loops and MS2-GFP-NLS by lipofectamine 2000 (Invitrogen). Cells were washed and fixed 24 h post-transfection with 4% parafolmaldehyde, permeabilized with 0.1% TritonX100, and stained with mouse anti-p24 antibody (AG3.0, Dr. Jonathan Allan, NIH AIDS Reagent Program, # 4121, 1:250) followed by staining with Alexa594-conjugated anti-mouse-IgG antibody (Invitrogen, # A-11020, 1:200) and DAPI (Sigma). Cells were mounted on a slide glass with Fluoromount-G (Southern Biotech) and imaged with a confocal microscopy (Leica SP5). For co-localization analysis, more than 50 cells were imaged, and correlation between p24 signals and GFP signals in cytosol (DAPI negative) were analyzed with ImageJ (NIH).
Akiyama H., Miller C.M., Ettinger C.R., Belkina A.C., Snyder-Cappione J.E, & Gummuluru S. (2018). HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction. Nature Communications, 9, 3450.
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4

Myotube Differentiation and Quantification

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The muscle cells were plated on 24 well plates (30,000 cells/well) in DMEM supplemented with 2% FBS to promote myogenic differentiation (myotube formation). Three days after plating, immunocytochemical staining for fast myosin heavy chain (MyHCf) was performed. After rinsing two times with PBS, cells were fixed for 5 min in cold methanol (− 20 °C), blocked with 10% donkey serum (017-000-121, Jackson ImmunoResearch) for 1 h, and then incubated with a mouse anti-MyHCf (M4276, 1:250, Sigma-Aldrich, St. Louis, MO, USA) antibody for 2 h at RT. The primary antibody was detected with an Alexa 594-conjugated anti-mouse IgG antibody (A21203, 1:500, Invitrogen, Waltham, MA, USA) for 30 min. The nuclei were revealed by 4, 6-diamidino-2-phenylindole (DAPI, D9542, 100 ng/ml, Sigma-Aldrich, St. Louis, MO, USA) staining. The percentage of differentiated myotubes was quantified by the number of nuclei in MyHCf positive myotubes relative to the total number of nuclei. All stained cells were visualized on a Nikon Eclipse fluorescence microscope.
Lu A., Tseng C., Guo P., Gao Z., Whitney K.E., Kolonin M.G, & Huard J. (2022). The role of the aging microenvironment on the fate of PDGFRβ lineage cells in skeletal muscle repair. Stem Cell Research & Therapy, 13, 405.
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