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Na2co3

Manufactured by Honeywell
Sourced in Germany, Belgium

Sodium carbonate (Na2CO3) is a chemical compound commonly used in laboratory settings. It is a white, crystalline solid that serves as a source of sodium ions and carbonate ions. Na2CO3 is a versatile compound with a variety of applications in analytical chemistry, pH adjustment, and various laboratory procedures.

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7 protocols using na2co3

1

Synthesis and Characterization of Iron Nanoparticles

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Hydrochloric acid (HCl) and l-(+)-Ascorbic acid were purchased from Merck (Darmstadt, Germany). Na2CO3 and Na2SO3 were purchased from Riedel de Haen (Seelze, Germany). Chloroform (CHCl3) was purchased from LAB-SCAN (Dublin, Ireland). Oleic acid and Iron(II) sulfate heptahydrate (FeSO4·7H2O) were purchased from Sigma Aldrich (Steinheim, Germany). Iron(III) chloride anhydrous was purchased from Fluka Chemicals (Buchs, Switzerland). NaOH was from Pronalab (Madalena, Portugal). Ethanol was from Panreac Quimica (Barcelona, Spain). The medium brain-heart infusion (BHI) and Bacteriological agar type E were purchased from Biokar Diagnostics (Beauvais, France).
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2

Antioxidant and Redox Activity Assays

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Thiobarbituric acid (TBA); lipoxygenase from soybean; nitroblue tetrazolium (NBT); reduced form of nicotinamide adenine dinucleotide (NADH) and N-phenylmethazonium methosulfate (PMS) were purchased from Fluka, Biochemika, Sigma-Aldrich, Steinheim, Germany. Linoleic acid sodium salt; 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS); 2,2-diphenyl-1-picrylhydrazyl (DPPH); nordihydroguaiaretic acid (NDGA) and 2-deoxyribose were purchased from Sigma-Aldrich, Steinheim, Germany. Trichloroacetic acid was purchased from VWR, Leuven, Belgium. Ferrozine iron reagent hydrate; ethylenediaminetetraacetic acid (EDTA); FeCl2.4H2O, FeCl3; and potassium persulfate were purchased from Acros organics, New Jersey, USA. L(+)-Ascorbic acid and boric acid were purchased from Merck, Darmstadt, Germany. KH2PO4-K2HPO4; FeSO4.7H2O were purchased from Panreac, Barcelona, Spain. H2O2 was purchased from Fisher Scientific, New Jersey, USA. L-α-lecithin of soybean was purchased from CALBIOCHEM, Darmstadt, Germany. Chloroform was purchased from lab-scan, Dublin; Folin Ciocalteu's phenol reagent, was purchased from Panreac Química SA (Barcelona, Spain). Gallic acid; Na2CO3; acetic acid; acetic acid; sodium dodecyl sulfate (SDS); KCl; butanol; butylated hydroxytoluene (BHT); mannitol KCl were purchased from Riedel de Haen (Seelze, Germany).
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3

Synthesis of Metal-Organic Compounds

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The Rhodamine B, etilenediamine (EDA), methyl acrylate, methanol, dimethyl sulfoxide (DMSO), FeSO4·7H2O, CuSO4·5H2O, PbSO4, CoCl2·6H2O, Sr(NO3)2, CaCl2·2H2O, Zn(NO3)2, Ag2SO4, Hg(NO3)2·H2O, CdSO4·2.67H2O were from Sigma Aldrich (Slovenia). MgCl2·6H2O, NaNO3 and KNO3 were provided by Alkaloid Skopje. NiCl2·6H2O, LiCl, AlCl3·6H2O and Mn(CH3COO)2·6H2O were purchased from Kemika (Slovenia). The Na2CO3 and NaHCO2 were provided by Riedel-de Haën (Slovenia). All of the reagents were used as received. The solvent used in synthesis was of analytical grade.
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4

Antioxidant Evaluation of Natural Oils

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Thiobarbituric acid (TCA), ferrous chloride (FeCl2), ammonium thiocyanate (NH4SCN), ferulic acid, DNP (2,4-dinitrophenylhydrazine), sulphuric acid (H2SO4), eryodictiol, quercetin, potassium hydroxide (KOH), N-methyl-N-(trimethylsilyl) trifluoroacetamide, NH4I, 2-mercaptoethanol, and buthylated hydroxyanisole (BHA) were purchased from Fluka Biochemika, Sigma-Aldrich, Steinheim, Germany. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was from Riedel-de Haën, Sigma-Aldrich, Seelze, Germany. Folin–Ciocalteu’s phenol reagent and AlCl3 were purchased from Panreac Quimica, Montcada i Reixac, Barcelona, Spain. Na2CO3 was purchased from Riedel de Haen (Seelze, Riedel-de-Haën Laboratory chemicals, Germany). Almond oil and wheat germ oil were purchased from LBCHEM Labospirit, Lda Portugal. Tween 80, Span 80, Phenonip and xanthan gum were purchased from Guinama, Valencia, S.L.U Spain. Trichloroacetic acid was purchased from VWR, Leuven, Belgium. 1-Butanol, methanol, and HCl were from Fisher Scientific UK Ltd., Loughborough, UK.
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5

Antioxidant Compounds Characterization

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Water was distilled and purified using a Stakpure GmpH water system (Niederahr, Germany). Gallic acid, ellagic acid, AlCl3, and Trolox were purchased from Acros Organics (Geel, Belgium). Chlorogenic acid was purchased from the HWI group (Rülzheim, Germany). Na2CO3 was obtained from Honeywell (Charlotte, NC, USA), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Kandel, Germany). Folin–Ciocalteu reagent was purchased from Fisher Scientific (Loughborough, UK). All solvents used were analytical or HPLC grade. Acetonitrile and formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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6

Phytochemical Extraction and Analysis

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The process of preparing the extract involved the use of ethanol (96%) sourced from Kalsnavas Elevators Ltd. (Jaunkalsnava, Latvia). LC-MS grade acetonitrile, methanol, and formic acid were procured from Fisher Scientific (Loughborough, UK), while water used for LC-qTOF-MS analysis was purified via a Smart2Pure water purification system (Thermo Scientific, Dreieich, Germany). Gallic acid, cyclohexane, xylene, ascorbic acid, and chlorogenic acid were obtained from Acros Organics (Geel, Belgium), and Na2CO3 was sourced from Honeywell (Charlotte, NC, USA). In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phenol were purchased from Alfa Aesar (Kandel, Germany). We used Folin–Ciocalteu reagent, H2SO4, HNO3, HCl, and D-glucose, all of which were purchased from Fisher Scientific (Loughborough, UK). Standards of rosmarinic acid and leucine were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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7

Phytochemical Analysis of Plant Extracts

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Reference standards: caffeic acid (≥98%), quinic acid (≥98%), catechin (≥98%), apigenin (≥95%), and apigenin-7-glucoside were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rutin was purchased from PhytoLab (Vestenbergsgreuth, Germany), and chlorogenic acid was purchased from the HWI group (Rülzheim, Germany). All solvents used were analytical or HPLC grade. The water was distilled and purified using the Stakpure GmpH water system (Niederahr, Germany). Gallic acid and AlCl3 and Trolox were purchased from Acros Organics (Geel, Belgium), and Na2CO3 and NaNO2 were obtained from Honeywell (Charlotte, NC, USA). 2,2-diphenyl-1-picrylhydrazyl (DPPH) was from Alfa Aesar (Kandel, Germany). Folin–Ciocalteu reagent, H2SO4, HCl, and NaOH reagents were purchased from Fisher Scientific (Loughborough, UK). LC-grade acetonitrile, methanol, and formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA), and water for LC analysis was purified using a Stakpure GmpH water purification system (Niederahr, Germany).
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