High fidelity herculase 2 dna polymerases

Primary antibodies against p110δ, β-actin, Akt, and p-Akt were purchased from Cell Signaling Technology (Danvers, MA), and an antibody against dCasX was ordered from Hangzhou Huaan Biotechnology Co., Ltd (Hangzhou, Zhejiang, China). Alexa fluorescence-488-conjugated anti-CD11β antibody was purchased from Abcam (Cambridge, UK), Alexa fluorescence-532-conjugated anti-Panck antibody was from Nanostring (Nanostring Technologies, Seattle, WA), and Alexa fluorescence-594-conjugated mouse endothelial-specific IB4 was purchased from Life Technology (Grand Island, NY). Alexa fluorescence-488-conjugated mouse anti-rabbit IgG and Alexa fluorescence-594-conjugated mouse anti-rabbit IgG were ordered from Hangzhou Huaan Biotechnology Co., Ltd. Horseradish peroxidase-conjugated mouse anti-rabbit IgG and goat anti-mouse IgG were purchased from Santa Cruz Biotechnology (Dallas, TX). Enhanced chemiluminescent substrate to detect horseradish peroxidase was purchased from Thermo Scientific (Waltham, MA). The plasmid of pAAV-RSV-SpCas9 was from our own laboratory deposited to Addgene (Cat. 85450, Cambridge, MA).⁵² (link) High-fidelity Herculase II DNA polymerases were from Agilent Technologies (Santa Clara, CA).