Alexa fluo488 chicken anti rabbit igg h l a21441

SP600125 was obtained from Calbiochem (420119, Darmstadt, Germany). EX527 (203044) and sirtnol (s7942) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
Antibodies that recognize tyrosinase (sc7833) and GAPDH (sc-25778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies recognize total (phosphorylated and non-phosphorylated) ERK1/2 (9102), phospho-specific ERK1/2 (Thr202/Tyr204, 9101S), FoxO3a (12829S), and SirT1 (8469s) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody to MITF (MS-771-PI) was purchased from ThermoScientific Nunc (Rochester, NY, USA). For confocal staining, Alexa Fluo488 chicken anti-rabbit IgG (H + L) (A21441, Molecular Probes, Eugene, OR, USA), and Alexa Fluo555 donkey anti-goat IgG (H + L) (A21432, Molecular Probes, Eugene, OR, USA) were obtained from Invitrogen (Carlsbad, CA, USA).

Antibodies that recognize p63 (sc-8343) and integrin α6 (sc-6597) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Alexa Fluo488 chicken anti-rabbit IgG (H + L) (A21441, Molecular Probes) and Alexa Fluo555 donkey anti-goat IgG (H + L) (A21432, Molecular Probes) were obtained from Invitrogen (Carlsbad, CA, USA). Epidermal growth factor (EGF) was also obtained from Invitrogen (Carlsbad). Normal goat serum (5%, S-1000, VECTOR, Burlingame, CA, USA) was used for blocking. Diphenyl-2-picrylhydrazyl (DPPH), 2′,7′-dichlorofluorescin diacetate (H₂DCFDA), bovine serum albumin (BSA), and H₂O₂ were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Keratinocyte growth medium (KGM) and keratinocyte basal medium (KBM) were obtained from Lonza (Basel, Switzerland). Dulbecco's modified Eagle's medium (DMEM, LM001-05) was obtained from WelGENE (Daegu, South Korea). Fetal bovine serum (FBS, HyClone) was from ThermoScientific (Logan, UT, USA) and 4′,6-diamidino-2-phenylindole (DAPI, 10236276001) was obtained from Roche (Basel).