Scid beige cb17 cg prkdcscidlystbg 1 crl

The PDX models used in this study WHIM20 (isolated from a patient who developed the Y537S mutation) and WHIM43 (D538G mutation) were obtained from Washington University in St. Louis (Horizons Discovery Group, Waterbeach UK). All animal procedures were reviewed and approved by Tulane University IACUC. SCID/beige (CB17.Cg-Prkdc^scidLyst^bg-1/Crl) 4–6-week-old female mice were obtained from Charles River Laboratory. Intact tumor pieces were removed and sliced with a scalpel to 3mm x 3mm and coated with 100 uL phenol-free growth factor reduced Matrigel (BD Biosciences). Control groups had PDX tumor coated in Matrigel and implanted bilaterally in the mammary fat pads under isoflurane and oxygen. In ASC groups, 10⁶ pooled donors (n = 6) of lnASCs or obASCs were resuspended in Matrigel and coated the tumor. Where indicated, estradiol pellets were implanted subcutaneously in the lateral area of the neck (0.72 mg, 60-day release; Innovative Research of America). Tumors were implanted into the fifth mammary fat pad bilaterally under isoflurane and oxygen anesthesia delivered by nose cone and animals were given 5mg/kg/day meloxicam for three days post-surgery. Tumors were measured by digital caliper every three to four days. At endpoint (tumors reach 750–1000 mm³) blood and lungs were collected for analysis. PDX derived cells were cloned out from PDX tumors.

The PDX model used in this study, TU-BcX-2 K1, was derived from the biopsy specimen of an African-American patient that had node negative invasive ductal carcinoma at the time of biopsy. Tumor tissue was obtained through the Louisiana Cancer Research Consortium (LCRC) Biospecimen Core in compliance with NIH regulations and institutional guidelines and approved by the Institutional Review Board at Tulane University and LCRC. All animal procedures were reviewed and approved by Tulane University IACUC. SCID/beige (CB17.Cg-Prkdc^scidLyst^bg-1/Crl) 4–6-week-old female mice were obtained from Charles River Laboratory. Intact tumor pieces were removed and sliced with a scalpel to 3 mm × 3 mm and coated with 100 μL phenol-free growth factor reduced Matrigel (BD Biosciences). In indicated groups, 10⁶ pooled donors (n = 6) of lnASCs or obASCs were resuspended in Matrigel and coated the tumor. Tumors were implanted into the mammary fat pads bilaterally under isoflurane and oxygen anesthesia delivered by mask and animals were given 5 mg/kg/day meloxicam for 3 days post-surgery. Tumors were measured by digital caliper every three to 4 days. At endpoint (tumors reach 750–1000 mm³), blood, lungs, and tumor were collected for analysis.