PCR was performed using a PowerPlex ESX 17 and PowerPlex HS 16 kit (Promega Corporation, USA) in a Gene Amp PCR System 9700 thermocycler (Applied Biosystems, USA). Amplification products were separated towards DNA CC5 ILS 500 and CC5 ILS 600 standards (Promega Corporation, USA) using a 3130 Genetic Analyzer (Applied Biosystems, USA). The following loci were analyzed: AMEL, D3S1358, TH01, D21S11, D18S51, D10S1248, D1S1656, D2S1338, D16S539, D22S1045, VWA, D8SS1179, FGA, D2S441, D12S391, D19S433, SE33, D5S818, D13S317, D7S820, TPOX, CSF1PO, Penta D, and Penta E. Additionally, alleles from chromosome Y were determined using a Yfiler test (Applied Biosystems, USA). PCR products were analyzed in a 3130 Genetic Analyzer (Applied Biosystems, USA). Genotypes were generated using Gene Mapper ID v3.2 software (Applied Biosystems, USA). Multiplex PCR and capillary electrophoresis procedures were executed according to the manufacturer’s recommendations.
Cc5 ils 600
Manufactured by Promega
Sourced in United States
The CC5 ILS 600 is a laboratory instrument designed for automated liquid handling. It features a 600 μL dispensing volume and can perform various liquid transfer tasks, including aspiration, dispense, and mixing, to facilitate efficient sample processing and preparation in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Powerplex 16 hs, by Promega (2 mentions)
3130 genetic analyzer, by Thermo Fisher Scientific (2 mentions)
Powerplex esx 17, by Promega (2 mentions)
Genemapper id v3, by Thermo Fisher Scientific (1 mentions)
Geneamp pcr system 9700, by Thermo Fisher Scientific (1 mentions)
Geneamp pcr system 9700 thermocycler, by Thermo Fisher Scientific (1 mentions)
2 protocols using cc5 ils 600
1
Multiplex STR Profiling Using PowerPlex Kits
2
Forensic DNA Degradation Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The qualitative analysis was performed using sequences for T. Large (214 bp) and T. Small (80 bp) autosomal chromosome as well as for chromosome Y (75 bp) (for male cadavers). Degradation index was calculated based on the ratio of T. Small to T. Large autosomal sequences. The PCR reaction was performed using a Power Plex ESX 17 and Power Plex HS 16 kit (Promega Corp., USA) in a Gene Amp PCR System 9700 thermocycler (Applied Biosystems, USA). Amplification products were separated toward DNA CC5 ILS 500 and CC5 ILS 600 standards (Promega Corp., USA) using 3130 Genetic Analyzer (Applied Biosystems, USA).
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