Female NOD mice were intravenously injected with 100 µg anti-Thy1.2 or control rat IgG at 4 weeks of age. Urine was tested for glucose weekly using test strips (Uropaper III; Eiken Chemical, Tokyo, Japan). Mice were sacrificed at 3 months of age and their pancreata were fixed with 10% formaldehyde and stained with hematoxylin and eosin (HE) by GenoStaff, Tokyo, Japan. The histological severity of insulitis was semi-quantitatively scored as 0 (intact islets), 1 (mild insulitis with limited cellular infiltration), 2 (moderate insulitis with cellular infiltration and mild destruction of islets), 3 (severe insulitis with extensive cellular infiltration and destruction of islets) or 4 (severe destruction of islets).
Uropaper 3
Manufactured by Eiken Chemical
Sourced in Japan
Uropaper III is a laboratory equipment used for the analysis of urine samples. It provides a standardized method for performing qualitative and semi-quantitative tests on urine specimens.
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Lab products found in correlation
7 protocols using uropaper 3
1
Monitoring Autoimmune Diabetes in Mice
2
Quantifying Urinary Protein Levels
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Urinary protein concentrations were measured using a fluorescence-based protein assay for total proteins (EZQTM Protein Quantitation Kit, Thermo
Fisher Scientific, Waltham, MA, USA) and dipsticks for urinalysis (Uropaper III, Eiken, Tokyo, Japan).
Fisher Scientific, Waltham, MA, USA) and dipsticks for urinalysis (Uropaper III, Eiken, Tokyo, Japan).
3
Evaluating Adverse Effects of High-Dose Probucol
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To evaluate adverse effects of high dose probucol we measured 13 biomarkers using blood samples. Erythrocyte-, leukocyte-, and platelet-counts in blood samples were measured by using an automatic blood cell counter (Celltac α MEK-6458, Nihon Kohden, Tokyo, Japan). Blood aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), blood urea nitrogen, and their corresponding levels in serum were determined using CicaLiquid AST, CicaLiquid ALT, CicaLiquid LDH J, CicaLiquid-N UN (Kanto Chemical Co., Inc., Tokyo, Japan), respectively. Serum creatinine levels were determined using Determiner L CRE (Kyowa Medex Co., Ltd., Tokyo, Japan). Total protein levels in serum were determined using Clinimate TP (Sekisui Medical Co., Ltd., Tokyo, Japan). Serum concentrations of sodium, potassium and chloride were measured using Bio Majesty JCA-BM8060 (JEOL, Tokyo, Japan). Plasma cortisol levels were measured using electrochemiluminescence immunoassay (ECLIA, Roche Diagnostics, Mannheim, Germany).
Urine samples were analyzed for leukocyte, urobilinogen, occult blood reaction, bilirubin, ketonic metabolite, glucose, protein and pH values by using dipstick urinalysis (Uropaper III, Eiken Chemical Co., Ltd., Tokyo).
Urine samples were analyzed for leukocyte, urobilinogen, occult blood reaction, bilirubin, ketonic metabolite, glucose, protein and pH values by using dipstick urinalysis (Uropaper III, Eiken Chemical Co., Ltd., Tokyo).
4
Probiotic Milk Replacer Fermentation
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A probiotic mixed feed BIO-THREE Ace (Toa Biopharma, Tokyo, Japan) including Streptococcus faecalis T-110 strain (1 × 10 8 cfu/g), Clostridium butyricum TO-A strain (1 × 10 6 cfu/g), and Bacillus mesentericus TO-A strain (1 × 10 6 cfu/g) was added to milk replacer (GREATBABY; Nosan Corporation, Yokohama, Japan) prepared at 3.5and 7-fold concentrations. The 1% mixtures were incubated at room temperature (22-25°C) for 5 days, and the pH, glucose, and number of bacteria were examined chronologically. The pH of the mixture was measured using a pH meter (AS-600; AS ONE, Osaka, Japan). The glucose in the mixture was evaluated using Uro Paper III (Eiken Chemical, Tokyo, Japan). The bacteria in the mixture were isolated by using 5% sheep blood agar plates (Becton Dickinson, Franklin Lakes, NJ, USA) at 37°C overnight and then identified morphologically, and the numbers of bacteria were counted.
5
Measuring Diabetes in Mice
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Urine glucose levels were measured semiquantitatively using Uro‐paper III (Eiken, Tokyo, Japan). To obtain paired measurements with urine glucose levels, blood was collected by tail snip immediately after urine collection. The blood glucose levels were measured using Glutest Ace and Glutest Sensor (Sanwa Chemical Co., Tokyo, Japan). Diabetes was diagnosed when the glucose level was >250 mg/dL under ad libitum feeding conditions.
6
Urine Analysis: Fundamental Techniques
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Urine collected by manual compression of the urinary bladder was centrifuged (1500 g, 5 min), and the supernatant was then analyzed for urine-specific gravity by a refractometer (UR-JE, ATAGO, Tokyo, Japan).
Testing for the presence of hemoglobin, bilirubin, urobilinogen, ketones, proteins, glucose and nitrite was performed using urine test strips (Uropaper III, Eiken Chemical, Tokyo, Japan).
Testing for the presence of hemoglobin, bilirubin, urobilinogen, ketones, proteins, glucose and nitrite was performed using urine test strips (Uropaper III, Eiken Chemical, Tokyo, Japan).
7
Severe Burn-Induced Coagulation Abnormalities
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Urine samples were obtained by bladder puncture immediately after exsanguination. We determined the presence of hematuria by Uro-Paper III (EIKEN Chemical CO., LTD., Tokyo, Japan). The degree of hematuria was interpreted as "none (-)," "mild (+/-or +)," and "severe (++ or +++)," respectively. We also checked the level of free Hb (colorimetric methods; BML, Inc., Tokyo, Japan) in the plasma to evaluate severe burn-induced hemolysis.
Plasma thrombin-antithrombin complex (TAT; enzyme immunoassays; BML, Inc., Tokyo, Japan) and plasmin-α2 plasmin inhibitor complex (PIC; latex agglutination; BML, Inc., Tokyo, Japan) values were measured in order to confirm hypercoagulability and hyperfibrinolysis in the severe burn model.
Plasma thrombin-antithrombin complex (TAT; enzyme immunoassays; BML, Inc., Tokyo, Japan) and plasmin-α2 plasmin inhibitor complex (PIC; latex agglutination; BML, Inc., Tokyo, Japan) values were measured in order to confirm hypercoagulability and hyperfibrinolysis in the severe burn model.
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