Sodium selenite, fluorescein isothiocyanate-dextran (FITC-dextran) with average molecular weight 4,000 and H2O2 were purchased from Sigma-Aldrich Co.. Nrf2 inhibitor (ML385) was purchased from MedChemExpress (Shanghai, People's Republic of China). All reagents for cell culture were purchased from Life Technologies (Thermo Fisher Scientific, Waltham, MA, USA). Mito-Tracker Green Kit, ATP Assay Kit, MMP Assay Kit, ROS detection Kit, and Cell Counting Kit-8 (CCK-8) were purchased from Beyotime Biotechnology (Shanghai, People's Republic of China). RIPA lysis buffer and bicinchoninic acid (BCA) protein assay kit were purchased from Solarbio Life Sciences Biotech Co. (Beijing, People's Republic of China). Nrf2, NQO-1, and HO-1 primary antibodies and corresponding secondary antibody were purchased from Abcam Biotechnology (Cambridge, MA, USA). Genomic DNA extraction Kit was purchased from OMEGA Bio-Tek (Norcross, GA, USA). ChamQTM SYBR® qPCR Master Mix Kit was purchased from Vazyme (NanJing, People's Republic of China).
Nrf2 inhibitor ml385
Manufactured by MedChemExpress
Sourced in China
Nrf2 inhibitor (ML385) is a laboratory reagent used in research applications. It functions as a potent and selective inhibitor of the Nrf2 transcription factor. Nrf2 plays a key role in the regulation of antioxidant response elements. The inhibition of Nrf2 by ML385 has been studied in various in vitro and in vivo models.
Automatically generated - may contain errors
Lab products found in correlation
Mito tracker green kit, by Beyotime (1 mentions)
Chamq sybr qpcr master mix kit, by Vazyme (1 mentions)
Fitc dextran, by Merck Group (1 mentions)
Genomic dna extraction kit, by Omega Bio-Tek (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Ros detection kit, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Mmp assay kit, by Beyotime (1 mentions)
Arpe 19, by American Type Culture Collection (1 mentions)
2 protocols using nrf2 inhibitor ml385
1
Nrf2-mediated Antioxidant Response Assay
2
Establishing Diabetic Retinopathy Cell Model
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The human retinal pigment epithelial cell lines ARPE-19 were got from American Type Culture Collection (Manassas, VA, USA). The cell culture dishes required here were Dulbecco’s modified Eagle’s medium/nutrient mixture F12 (Procell, Wuhan, China) with 10% fetal bovine serum (Rwdls, Shenzhen, China) and 100 U/mL penicillin/streptomycin. These mediums were placed in a suitable incubator with 95% air and 5% CO2 at 37°C. For the experiments, some of the dishes were added with 30 mM high glucose (HG) for 9 days incubation to establish DR cell model [16 ]. Different concentrations of CAR were added to the established cell model for subsequent experimental studies. The other part was handled only by CAR at the doses of 2.5, 5, 10, 20 μM [15 (link),17 (link)] for 2 h. CAR was supplied by Qilu Pharmaceutical (Jinan, China). Cells were pretreated with 10 μmol/L Nrf2 inhibitor ML385 (MedChemExpress, Shanghai, China) for 24 h and stored for the later experiments.
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