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Monoclonal mouse

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The Monoclonal mouse is a laboratory animal used in biomedical research. It is genetically engineered to produce a single type of antibody, known as a monoclonal antibody. Monoclonal antibodies are important tools for studying specific proteins and their functions in biological systems.

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Lab products found in correlation

Horseradish peroxidase conjugated anti mouse antibody, by Agilent Technologies (1 mentions)

2 protocols using monoclonal mouse

1

Quantitative Western Blotting of Neuronal Markers

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Western blotting was performed as described by Krabbe et al. [59 (link)]. Membranes were incubated (over night/4°C) with anti-TH (1:2000; monoclonal mouse; Chemicon) or anti-β-tubIII antibody (1:2000; monoclonal mouse; Sigma) diluted in TBS/Tween-20, washed, incubated (1 hr) with horseradish peroxidase-conjugated anti-mouse antibody (1:2000; DAKO) diluted in TBS/Tween-20, developed with chemiluminiscence (SuperSignal®Extended duration substrate; Thermo Scientific), and visualized using a charge coupled device camera. Loading control: alpha-actin antibody (1:6000; mouse; Chemicon).
Dreyer-Andersen N., Almeida A.S., Jensen P., Kamand M., Okarmus J., Rosenberg T., Friis S.D., Martínez Serrano A., Blaabjerg M., Kristensen B.W., Skrydstrup T., Gramsbergen J.B., Vieira H.L, & Meyer M. (2018). Intermittent, low dose carbon monoxide exposure enhances survival and dopaminergic differentiation of human neural stem cells. PLoS ONE, 13(1), e0191207.
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2

Immunogold Labeling of Optic Nerve

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Immunogold labeling of cryosections was performed as described [52 (link)] on optic nerves of postnatal day 75. Antibody was specific for acetylated α-tubulin (1:1000, monoclonal mouse, Sigma, T 6793) and was detected by incubation with rabbit anti-mouse IgG secondary antiserum (1:200, Rockland Immunochemicals, Limerick, PA USA, #110-4102) which was visualized with protein A-gold (10 nm) (CMC, Utrecht, The Netherlands).
Kusch K., Uecker M., Liepold T., Möbius W., Hoffmann C., Neumann H., Werner H.B, & Jahn O. (2017). Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome. Proteomes, 5(1), 3.
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