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Dr1900 portable spectrophotometer

Manufactured by HACH
Sourced in United States

The DR1900 Portable Spectrophotometer is a compact and versatile laboratory instrument designed for water analysis. It provides accurate measurements of various water quality parameters, including turbidity, color, and concentration of specific compounds. The instrument features a simple and user-friendly interface, making it suitable for field or laboratory use.

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6 protocols using dr1900 portable spectrophotometer

1

Wastewater Treatment Process Analysis

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COD, MLSS, MLVSS and SVI parameters were determined as stated by standard methods [21 ]. A DR1900 Portable Spectrophotometer (HACH, USA) was used for measuring COD values. Excessive amounts of chlorine ion result in a substantial error in determination of COD value. Therefore, in this study a modification of standard methods was used and sufficient amount of mercury sulfate was added to the samples before COD tests [22 (link)–24 (link)], and. The operational parameters namely DO, pH, Temperature and Electric Conductivity (EC) were measured by a digital multi-meter (SensoDirect 150, Germany).
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2

Analytical Techniques for PAA and HP Measurement

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All solutions were prepared using >18 MΩ-cm deionized water from an Evoqua water purification system. PAA solution (32% w/w of PAA, 40–45% w/w of acetic acid, and <6% w/w of H2O2), HP solution (30.9% w/w), and Tygon tubing were supplied by Sigma Aldrich (St. Louis, MO). A V-2000 photometer and PAA and HP Vacu-vials instrumental test kits (K-7913, K-5543) were purchased from Chemetrics (Midland, VA). A DR1900 portable spectrophotometer and DPD total chlorine reagent powder pillows were purchased from HACH (Loveland, CO). 50-mL glass impingers were purchased from Ace Glass (Vineland, NJ). Plastic impingers were purchased from SKC Inc. (Eighty Four, PA). 25 mm Acrodisc syringe filters with 0.45 μm PTFE membranes were purchased from Pall Corporation (Port Washington, NY). The 3D printed nozzle was printed using an Objet Eden 260vs 3D printer with Veroclear and SUP 705 was used as support material. The support material was washed away before experimental use. Flow-limiting critical orifices (1 L/min) and Aerosol Adapter connectors were purchased from Millipore (St. Louis, MO). A Bios DryCal Defender flowmeter from Mesa Labs (Lakewood, CO) was used to measure the orifice flow rate.
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3

In-Situ Water Quality Analysis

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For each sampling site, temperature, pH, and dissolved oxygen (DO) level were measured in situ using an HQ40D portable multi-parameter water quality analyzer (HACH, Loveland, CO, United States). Turbidity was measured using a 2100Q portable turbidimeter (HACH, Loveland, CO, United States). Ammonia-nitrogen, chemical oxygen demand (COD), and total organic carbon (TOC) were measured using a DR1900 portable spectrophotometer (HACH, Loveland, CO, United States). Adenosine triphosphate (ATP) level was measured with an ATP assay kit (Beyotime, Shanghai, China). All determinations were performed in triplicate.
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4

Nitrite Measurement in Strain MX-11

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Strain MX‐11 was grown on CGXIIMX supplemented with or without 60 mM nitrate. The concentrations of nitrite in cultures were measured using the TNT840‐CN Nitrite Test Kit (HACH, Loveland, CO, USA) with the HACH DR1900 Portable Spectrophotometer (HACH).
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5

Comprehensive Analytical Techniques for Water Quality

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Sample pH was measured
with a pH meter (FiveEasy F20 or SevenDirect SD20, Mettler Toledo,
Columbus, OH) and sample conductivity was measured with a pH/conductivity
meter (SevenDirect SD23, Mettler Toledo, Columbus, OH). TAN concentrations
were determined by the indophenol method using flow-injection analysis
(AA500 AutoAnalyzer, SEAL Analytical, Mequon, WI). A Dionex ICS-6000
was used to determine cation concentrations (IonPac SCS1 column, unsuppressed,
4 mM tartaric acid/2 mM oxalic acid eluent, 1.0 mL/min, 30 °C,
Thermo Scientific, Waltham, MA) and anion concentrations (IonPac AS22-4
μm column, suppressed, 4.5 mM Na2CO3/1.4
mM NaHCO3 eluent, 1.0 mL/min, 30 °C, Thermo Scientific,
Waltham, MA). Chemical oxygen demand (COD) was determined colorimetrically
by Hach Method 8000 using high-range (0–1,500 mg/L) reagent
kits (CHEMetrics, Midland, VA) with a DRB200 digital reactor block
and a DR1900 portable spectrophotometer (Hach Company, Loveland, Colorado).
Total organic and inorganic carbon (TOC and TIC) were measured using
a Shimadzu TOC-L autoanalyzer (Shimadzu Scientific Instruments, Inc.,
Columbia, MD).
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6

Water Quality Measurement Protocol

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Temperature, pH, conductivity,
and dissolved oxygen were measured using the Orion Star A325 pH/Conductivity
Portable Multiparameter Meter (Thermo Scientific, catalog no. STARA3250).
Total chlorine was measured using HACH Method 8167 (HACH, catalog
no. 2105669). Ammonium, nitrate, and nitrite were measured using the
Nitrogen-Ammonia Reagent Set (method 10023, HACH, catalog no. 2604545),
NitraVer X Nitrogen-Nitrate Reagent Set (method 10020, HACH, catalog
no. 2605345), and NitriVer 3 TNT Reagent Set (method 10019, catalog
no. 2608345), respectively. All HACH measurements were performed on
the DR1900 Portable Spectrophotometer (HACH, catalog no. DR190001H).
For metal analysis, samples were acidified with 2% nitric acid and
digested for a minimum of 16 h before being analyzed via inductively
coupled plasma mass spectrometry (ICP-MS) according to method 3125
B.28 Blanks and spikes of known concentrations
were measured every 10 samples for quality control. Samples for dissolved
organic carbon (DOC) and total dissolved nitrogen (TDN) were acidified
to pH <2 using HCl before being analyzed using a Shimadzu TOC-VCPH total organic carbon analyzer with a total nitrogen module
attached.29 (link) Certified DOC and TDN quality
references from ULTRA Scientific (Agilent, catalog nos. QCI-731 and
QCI-745) were measured to confirm precision and accuracy.
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